FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n01_S1_R2_A03_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n01_S1_R2_A03_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTTGAGGCTCTCATGGAATGGCTAAAGACAAGACCACTATTGTCACCT13.571428571428571No Hit
ATTCTACATCCAAATGTGCACCGAGCTTAAACTCAATGATTATGAGGGAC13.571428571428571No Hit
AGAGATATGGCCCAGCATTAAGCATAAATGAACTGAGCAATCTTGCAAAA13.571428571428571No Hit
AGTATCAGATGGAGGACCAAACTTATACAATATACGGAATCTTCACATTC13.571428571428571No Hit
AAATTCAGTGGTCTCAGCACCCTACAATGCTAGACAATAAAATGGAATTT13.571428571428571No Hit
CCAAAGATCACTGAGCAAAATGTGCAAAACACACAAACAAAAAATAATCC13.571428571428571No Hit
TTTTTATTTAAGTTTTCCATCCTTCTTTCTAATTTGTTGAATTATTTGCC13.571428571428571No Hit
TCCCAAGGACAAAGAACATGAAGAGAACAAGCCAATTGAAGTGGGCACTC13.571428571428571No Hit
GTCGTATCAGTAGGGCGTGGTTTGTCTCCGAAACTCCCACTGCAGATGGC13.571428571428571No Hit
CTATCATATTGCTTCAAATCGCTTATATCATTACAGTCGTCAAACTCAAC13.571428571428571No Hit
TTGAAGCTGAGTCCTCTGTCAAGGAGAAAGACATGACAAAAGAGTTTTTT13.571428571428571No Hit
GAAGTACTCTTGTCCCTCTGATGAAGCTTGATCCTCTTAGATCTTCAAAT13.571428571428571No Hit
ATGCATTAAGCAAAACCCAGGGATCATTAATCAGGCACTCCTCGATTGCT13.571428571428571No Hit
GAAATTTGCTATGGCTGACGAAGAATAATCGCAAGTACCCAAATCTGATC13.571428571428571No Hit
AAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGAGCTTC13.571428571428571No Hit
GGAAAGACCCTTTCAAACTGCTTCAAACAAGCCAATTGTACAGACTAATC13.571428571428571No Hit
ACATCTCAGTGCTTGGGGTCATGTCGGGCAGTATTAAGATCATTCCCATC13.571428571428571No Hit
GGTCTATCCCTACCAAAGAGTATCCCGCTTTTTCGAAGTAGTACCCACTG13.571428571428571No Hit
ACAAAATCATTAAATGGGTATCATAAAATAACCAATTAAAACAAAAACAT13.571428571428571No Hit
GTGGTAATGCCAGCCCATGGTCCAGCCAAAAGCATGGAATATGATGCCGT13.571428571428571No Hit
GGCCATTAATCTCCCACATCATTGCTGAAGATTAAGTTTTTGTTAGTTTC13.571428571428571No Hit
GTTTACACCGTCGGTGTAATTCATTAAGTGAGCTCTTCCTTTTATGAGGA13.571428571428571No Hit
GCGCGAATCATCGGTTGAATCCCATGCATCAACTTTTGAGGCATTTTCAG13.571428571428571No Hit
CCCAATAAGTGCATCACATTTCTGGGCACTTACCTAAGAAAACAGTTCTA13.571428571428571No Hit
CTATTGAAGGCCAATCGGAGAGTCACCCAGGGGAGTGGAGGAAGGCTCTA13.571428571428571No Hit
AGTAGAAACAAGGAGTTTTTTTTCAACGGACTACTTGTCAATGGTGAATG13.571428571428571No Hit
GATGAGTCGTCTCACCGAGGTCGAAACGTACGTTCTCTCTATCGTCCCGT13.571428571428571No Hit
GACTTACCCTTTCAATAATCATTATGATTTCTGAACTCATCTATCATTTT13.571428571428571No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)