FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n01_S1_R2_A01_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n01_S1_R2_A01_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAAAGTCTTCCCCGATAATCATCATCCATTAGCTCCCATTTTAAGCAGA14.0No Hit
CTTTTCAAACCGTATTTAAAGCGACGATAAATACATTTGAAAAAAAGACG14.0No Hit
AGAAAAAGGAGAGTAAGAGACAACATGACCAAGAAGATGGTCACGCAAAG14.0No Hit
CCTCCGATGAGGACCCCAACTGCATTTTTGACATCCTCATAAGTATGTCC14.0No Hit
GTCCCTGTCTTATTTATATAGGACTTCTTTTTGCTCATGTTGATTCCCAC14.0No Hit
GGTTAGAGATCAAAGAGGGAACGTACTATTGTCTCCCGAAGAAGTCAGTG14.0No Hit
GGAGATTGATCCAGTTGATAGTAAGCGGGAGAGACGAGCAGTCAATTGCT14.0No Hit
ATTCGACACTAATTGATGTCCATCCGAATTCTTTTGGTCGCAGTCTTGCT14.0No Hit
GTTCACCACATCAGTATCATTTCTCAAATGAGACCTTCCTTTTATAATGT14.0No Hit
CAATATACGGAATCTTCACATTCCTGAAGTCTGCTTAAAATGGGAGCTAA14.0No Hit
GTCAGCAGACCCATTAGCATCTCTCTTGGAAATGTGCCACAGCACACAGA14.0No Hit
CATGAAGGACAAGTTAACTTCATTATGTTTGAATTATGATTTAGTCAATT14.0No Hit
AGTTGATAGTAAGCGGGAGAGACGAGCAGTCAATTGCTGAGGCAATAATT14.0No Hit
CCATTGTGAATTCTTCATACCCTTCATGTACTCTTATTTTCAGTGTTTGG14.0No Hit
TATATTACCTAGAGAAAGCCAACAAAATAAAATCTGAGAAGACACACATT14.0No Hit
GTGTTTATATTGAAGTGTTGCACTTAACCCAAGGGACGTGCTGGGAGCAG14.0No Hit
AGCCAAAGGGGAATTCTTGAGGATGAACAGATGTACCAGAAGTGCTGCAA14.0No Hit
CTCATTTTCCTGGCACGGTCAGCACTCATTCTGAGGGGATCAGTTGCACA14.0No Hit
GTTCAGATCGCCCCTAACTGCCTTGATCATGCAATCCTCCTGTGAGAATA14.0No Hit
ATAAATACATTTCAAAAAAAGACTATCAGTAATCCACAATAGCAGGTGAA14.0No Hit
GATAAAGAACATGATCGAGAAAGGTGACTTTACTGAGAACGAAATTGGTC14.0No Hit
CTGGAGAGGAGATGGCCACCAAAGCGGACTACACCCTTGACGAAGAGAGC14.0No Hit
GGCCTCATATACACCAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGG14.0No Hit
GGGTAGAGAAGCCTAAATTTCTTACAGAATTGTATGATTACAAATAGAAC14.0No Hit
GTCAAGATAGAAGAAGAAGTGCTAACGGGCAACCTCCAAACACTGAAAAT14.0No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)