FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n01_S1_R1_H12-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n01_S1_R1_H12-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1221315
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAG35370.2896058756340502No Hit
GCCTAAGGATGTCTACCATCCTAATTCCACCAATCTGTGTGCTGTGGCAC29580.24219795875756867No Hit
TCCTTAGGCAGAACCCAACAGAAGAGCAAGCCGTGGATATATGCAAGGCT28270.2314718152155668No Hit
ATTATATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATGTCGCA27800.2276235041737799No Hit
TTCATATACTGATGACCTCTTTATAGCCAACCTTTGTTCATGGCAGCCAG26120.21386783917335003No Hit
AACAAATACAGTTCATTATCCAAAAATCTACAAAACTTATTTTGAAAGAG25800.21124771250660151No Hit
GTATAGCATTGTAGGGTTCTGGGACCACTGAATTTTAACAGTTTCCCAGT24540.2009309637562791No Hit
ATTACATCCTGTGCCTCCTTGGCACTGAGATCTGCATGACCAGGATTTAT24520.2007672058396073No Hit
GTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACA23900.195690710422782No Hit
CATATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAAGGATC22680.18570147750580318No Hit
GGATGTAATCATGGAAGTTGTTTTCCCTAACGAAGTGGGAGCCAGGATAC22050.18054310313064195No Hit
CCCATATGTCCTTCCGAGTGAGAGACACAAAAAATTCCAACACACTATTG21820.17865988708891645No Hit
CTTTATAGCCAACCTTTGTTCATGGCAGCCAGCATATGGGCATATGTTGC20750.16989883854697602No Hit
CTTCATATCCCTCATGCACTCTTATCTTCAATGTTTGAAGATTGCCCGTA20230.1656411327135096No Hit
TGGTAATAGCGATGACTAATACGTAGATGTACTGCCAAGTAGGAAAGTCC20100.164576706255143No Hit
GATATATGCAAGGCTGCAATGGGACTGAGAATTAGCTCATCCTTCAGTTT20060.16424919042179945No Hit
GTATTGGCCTCTAATGGCCTTAGGTACTAAAGACTGAAATGGTTCAAATT20030.16400355354679177No Hit
GATGTATACTCCAGGAGGGGAAGTGAGGAATGATGATGTTGATCAAAGCT19770.1618747006300586No Hit
GTATTTGTTATTGGTCCATTCCTATTCCACCATGTCACAGCCAGAGGTGA19620.16064651625502022No Hit
ATTCTAGACGGGCCGGCCCCTGCGTGTGGCACGGGCGGCCGGGAGGGCGT18880.1545874733381642No Hit
TTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGT18790.15385056271314115No Hit
TAACAAATACAGTTCATTATCCAAAAATCTACAAAACTTATTTTGAAAGA18620.152458620421431No Hit
GTATACATCTGTTCCCAGCATGTTCCTTGAGTCAAATGCAACACTTCAAT18610.1523767414630951No Hit
ATCTTATCATGTCTGGATCCGCTGCATTAATGAATCAGGGGATAACGCAG18570.15204922562975154No Hit
ATTAATAACTAGTCAATAATCAATGTCGACCCAGGTGGCACTTTTCGGGG18020.1475458829212775No Hit
ACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAAT17910.14664521437958267No Hit
CTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGT17770.14549890896288017No Hit
ATGCTATACAATAAAATGGAATTTGAACCATTTCAGTCTTTAGTACCTAA17670.14468011937952124No Hit
GGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAA17530.14353381396281878No Hit
CAATTATTGCTTCGGCAATCGACTGTTCGTCTCTCCCACTCACTATCAGC17400.14246938750445218No Hit
CTAGCATACTTACTGACAGCCAGACAGCGACCAAAAGAATTCGGATGGCC16500.13510028125422188No Hit
GTAGTACATTTCCTCGTTGGTCCCGGATTCTCAAAAAACGGTCAATGCTC16410.13436337062919884No Hit
GAATAAAAGAACTAAGAAATCTAATGTCGCAGTCTCGCACCCGCGAGATA16150.13223451771246567No Hit
GCATATATCCACGGCTTGCTCTTCTGTTGGGTTCTGCCTAAGGATGTCTA15970.1307606964624196No Hit
GCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTG15800.12936875417070945No Hit
GATATGAAGAGTTCACAATGGTTGGGAGAAGAGCAACAGCCATACTCAGA15560.12740365917064803No Hit
GCATTAATGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGC15390.12601171687893786No Hit
TCAATGAACTGAGCAACCTTGCGAAAGGAGAGAAGGCTAATGTGCTAATT15370.12584795896226608No Hit
CCACTACCCTCTCCGTGCTGGAGTACTCATCTACACCCATTTTGCTGATT15270.12502916937890718No Hit
GTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCAT15150.12404662187887647No Hit
CCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTT15050.12322783229551755No Hit
CTCCAACATGTATGCAACCATCAAAGGAGAAATTTTGCAATCCTGGAGTT15040.12314595333718165No Hit
CTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGG14960.12249092167049451No Hit
TAATAAAACTTCTTCCCTTCGCAGCCGCTCCACCAAAGCAAAGTAGAATG14950.1224090427121586No Hit
CTTATCTTCAATGTTTGAAGATTGCCCGTAAGCACCTCTTCCTCTCTCTT14910.12208152687881504No Hit
TTTTTAAACTATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG14830.1214264952121279No Hit
GTCATGTACTGGGCATAATGCCAGGCGGGCCATTTACCGTCATTGACGTC14760.12085334250377666No Hit
TTATAATGGTTACAAATAAAGCAATAGCATCACAAATTTCACAAATAAAG14400.11790570000368455No Hit
CTATTGCAATGAAAATAAATTTCCTTTATTAGCCAGAAGTCAGATGCTCA14400.11790570000368455No Hit
GAATGAGGAATCCCCTCAGAACAGCGGACTCCACTCCAGCTGTGCCTTCA14310.11716878937866153No Hit
GCATTGTAGGGTTCTGGGACCACTGAATTTTAACAGTTTCCCAGTTTCTG14190.11618624187863082No Hit
GAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGG14160.11594060500362315No Hit
CTCTTATCTTCAATGTTTGAAGATTGCCCGTAAGCACCTCTTCCTCTCTC14090.1153674522952719No Hit
CTAATGGCCTTAGGTACTAAAGACTGAAATGGTTCAAATTCCATTTTATT13940.11413926792023352No Hit
CTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCT13890.11372987312855405No Hit
TCTCAGTGCCAAGGAGGCACAGGATGTAATCATGGAAGTTGTTTTCCCTA13880.11364799417021816No Hit
CCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGG13600.11135538333681319No Hit
GTTTAAAAACGACCTTGTTTCTACTAATAACCCGGCGGCCCAAAATGCCG13580.1111916254201414No Hit
GTACTACTGTCTCCCGAGGAGGTCAGTGAAACACAGGGAACAGAGAAACT13500.11053659375345427No Hit
GATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACAT13460.11020907792011071No Hit
GGATCAGACCGAGTGATGGTATCACCTCTGGCTGTGACATGGTGGAATAG13450.11012719896177481No Hit
GTTATTAATAGTAATCAATTACGGGGTCATTAGTTCATAGCCCATATATG13150.10767083021169806No Hit
CTACAATGCTATACAATAAAATGGAATTTGAACCATTTCAGTCTTTAGTA13070.1070157985450109No Hit
ATGTACTACTGTCTCCCGAGGAGGTCAGTGAAACACAGGGAACAGAGAAA13070.1070157985450109No Hit
GGGTAGTGGTGAGCATTGACCGTTTTTTGAGAATCCGGGACCAACGAGGA13050.10685204062833914No Hit
GAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTT12920.10578761416997252No Hit
GATAAAAGCAGTCAGAGGTGATCTGAATTTCGTCAATAGGGCGAATCAGC12890.10554197729496484No Hit
ATCCCAATCATTCCCATCACATTGTCGATAGGTTCAACTCCCCAATTTTG12820.10496882458661361No Hit
GTTAGTATCCTGGCTCCCACTTCGTTAGGGAAAACAACTTCCATGATTAC12760.10447755083659825No Hit
GAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTT12700.1039862770865829No Hit
CTCCAGGAGGGGAAGTGAGGAATGATGATGTTGATCAAAGCTTGATTATT12690.103904398128247No Hit
CAATAATTGTGGCCATGGTATTTTCACAAGAGGATTGTATGATAAAAGCA12620.10333124541989577No Hit
TTTCAGAAGGATGCGAAAGTGCTTTTTCAAAATTGGGGAGTTGAACCTAT12550.10275809271154454No Hit
CTCCTGGAGTATACATCTGTTCCCAGCATGTTCCTTGAGTCAAATGCAAC12500.10234869791986506No Hit
GTTCTGAGGGGATTCCTCATTCTGGGCAAAGAAGACAAGAGATATGGGCC12440.1018574241698497No Hit
GTATTGACCAACACTGATTCAGGACCATTAATCTCCCACATCATTGACGA12400.10152990833650614No Hit
TTATTGTATAGCATTGTAGGGTTCTGGGACCACTGAATTTTAACAGTTTC12370.10128427146149847No Hit
GTATGCAACCATCAAAGGAGAAATTTTGCAATCCTGGAGTTCTTCTTTCT12340.10103863458649079No Hit
TCCTCATTCTGGGCAAAGAAGACAAGAGATATGGGCCAGCACTAAGCATC12310.10079299771148312No Hit
CCTCAGAACAGCGGACTCCACTCCAGCTGTGCCTTCATCTGGGTCTTCAG12270.10046548187813956No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTTGCG301.5990076E-596.6739145
GTCGGCC451.0912117E-896.6739145
AGGTGTG1100.079.096825145
GCCCTGG406.661307E-572.505424145
CCAAACC406.661307E-572.505424145
AATCGCG406.661307E-572.505424145
TTTCGCG1009.094947E-1265.25488145
GCACACC1400.056.96855145
AACCGGC902.0489097E-856.393105145
TATATAT8050.055.8564152
GGTGTAT651.0933041E-555.773403145
CGCTGGG651.0933041E-555.773403145
GGTAATA5600.054.3924332
TCGATGG400.00604941454.37907145
AGAAATA805.8717524E-754.37907145
GAGAGTG1206.002665E-1154.372397
GCTCTAG2050.053.070131
GTAATAG5800.052.516833
TAGGGGC1950.052.0679663
TAGCGAT5850.052.0487867
Produced by FastQC (version 0.11.5)