FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n01_S1_R1_H08-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n01_S1_R1_H08-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences990953
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT20390.20576152451226246No Hit
CTATTAGAAGAGGCCTTATTTTCTCAATTTTCTTCTTTGTTGATTCATTG17710.17871685135420146No Hit
GAATAAGAGAGGCTATCTAATAAGAGCACTGACATTAAATACGATGACCA17600.17760680879920643No Hit
GTATATTGTCTTGGTGTATTTCTTTTGTCCAAGATTCAGTATCGAGACTC14130.14259001183709016No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA13910.14036992672710008No Hit
AAATAAGACAGGGACATTTGAATTCACAAGCTTTTTTTATCGCTATGGAT13720.138452580495745No Hit
TTCGTATACTTTGTTGAAACTTTAGCTAGGAGCATTTGCGAAAAGCTTGA13050.13169141220622976No Hit
TCCTTAGACAGAATCCAACTGAGGAACAAGCCGTAGACATATGCAAGGCA12990.13108593444895975No Hit
GTCTAAGGATGTCCACCATCCTTACTCCTCCAATCTGTGTGCTGTGGCAC12930.13048045669168973No Hit
ATATAAACACTGCCTGTTCCGCCGGCTACTGGGAGAAACCTTGTTTTACG12740.12856311046033467No Hit
CTTCTAATAGATGGCACAGCATCACTGAGTCCTGGGATGATGATGGGCAT12280.12392111432126447No Hit
AACTTATACAATATACGGAATCTTCACATTCCTGAAGTCTGCTTAAAATG11990.12099463849445938No Hit
GTATAAGTTTGGTCCTCCATCTGATACTAATAGCCCTACCTTTGATTGGG11850.11958185706082933No Hit
CTCTTATTCAGTCTTTGTTTTTTCTTCCCTATTGTTCTTTGCGTGACCAT11280.11382981836676412No Hit
GATTAGAGGTTTCGTATACTTTGTTGAAACTTTAGCTAGGAGCATTTGCG11110.11211429805449906No Hit
TCCCAGGACTCAGTGATGCTGTGCCATCTATTAGAAGAGGCCTTATTTTC11020.11120608141859402No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATTCG2600.058.553039
CGAAAGC1650.052.74173
AGCGAAA1550.051.465691
TACTATA1652.7284841E-1143.9514162
GCGAAAG1903.6379788E-1241.985171
GTAGAAA5700.040.712892
CCTTATA5650.038.5061072
GGATAGC1901.2914825E-1038.1683355
AGTAGAA6300.037.986581
ATATAGT4300.037.1031721
TTTAAAC5200.036.259923
GGCCGGG1001.3892027E-436.25992145
TATATTC3950.034.8829573
CGAGGCG2008.925781E-932.633926145
CAGCTCG2651.382432E-1030.0919429
CCCTTAT7000.030.0439341
GTCTAAG7550.029.7763581
GGTCGTT1503.092282E-1128.9976887
TTATACT7650.028.439154
TCTAAGG8250.028.1289082
Produced by FastQC (version 0.11.5)