FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n01_S1_R1_H07-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n01_S1_R1_H07-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences894353
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATAAAGGGACTAGGTTTGTTCTTGTACAAGAAAAAGAGCTAATTTTTT9140.10219678359663353No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACGG100.0070843627145.043623
AGCGAAA3150.0117.416271
TATGCGT259.375212E-487.026174
GCGAAAG6150.062.498472
CTTACGC805.86082E-754.391363
CGAAAGC7000.053.873353
CCGTATG752.5517316E-548.3289579
CTATTCG450.00964225748.3289539
CCGATCA657.3394406E-444.6113438
GCCGATC700.001057052241.424827
TACCGTA1053.8260478E-641.424827
GTCGTAC951.02698286E-438.1693731
TATACCG951.02698286E-438.1693735
TCTTACG1354.9588925E-737.60392
TACGCTT1209.543019E-636.2609065
TCATCGT1209.568665E-636.2467169
TTACGCT1251.2623339E-534.810474
TACATCG1251.2633014E-534.80658145
GGACCGT1153.1635145E-431.5188837
TACACCG1204.050561E-430.2174225
Produced by FastQC (version 0.11.5)