FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n01_S1_R1_H05_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n01_S1_R1_H05_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTATATATGTGATCATGGCCAAAAACATTCTAGGATTCTGATTTTCGTTC17.142857142857142No Hit
TATGGACAGAGCCGTTAAACTGTATAGAAAGCTTAAGAGGGAGATAACAT17.142857142857142No Hit
GTCCACATATATTACATAGAGAAAGCCAACAAAAAAAAATCTGAGAAGAC17.142857142857142No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAGCTATCTCACTGCATCTGTT17.142857142857142No Hit
CTTGGATCCTCAATGCTTAATTTTAAAGCATCCATCAGCAGGAATTTTGA17.142857142857142No Hit
GCCTTATTGAATTCATTTTGGACCCAGCTTGCTAGAGATCTGGGCTCTGG17.142857142857142No Hit
GATTTATGGTTCGCTCGTTACGCAGAGACATTAACCTGATCGTAGTGTTA17.142857142857142No Hit
GCCTTAGCTGTAGTGCTGGCCAGAACCATTCTGTTCTCATGTCTTATTAA17.142857142857142No Hit
ATTATTGCCTCAGCAATTGACTGCTCGTCTCTCCCGCTTACTATCAACTG17.142857142857142No Hit
GTTAGTGGGAATCAACATGAGCAAAAAGAAGTCCTATATAAATAAGACAG17.142857142857142No Hit
GATGAATACTCCAGCACGGAGAGAGTCTTAGTGAGTATTGAGAGATATTT17.142857142857142No Hit
AAAGAGAATTGGTCCGTAAAACAAGGTTTCTCCCAGTAGCCGGCGGAACA17.142857142857142No Hit
GGCATAAAGAGATTGATTCTGTTAACAATGCTGTGGTACTGCCCGCCCAT17.142857142857142No Hit
CAGCACTTCTGGTACATCTGATCATCCTCAAGACTTCCCCTATGTCTAGT17.142857142857142No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)