FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n01_S1_R1_H04-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n01_S1_R1_H04-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences831968
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATACAAGAGAGTAGATGGAAAGTGGATGAGGGAACTTGTCCTTTATGA27310.32825781760836953No Hit
CTTAATATGAGTGCAGACCGTGCCAGAAAGGTGAGATCTTCGATCTCAGC21690.260707142582407No Hit
CTCATAAGCAATCCTTGTTTTTCTTCCATTCTCACCCCTCCAGAAGTTTC18630.2239268818031463No Hit
CATTATGATCTGGCATTCCAATTTGAATGATACAACTTACCAGAGGACAA16780.20169044963267818No Hit
CCCATATACAAGAGAGTAGATGGAAAGTGGATGAGGGAACTTGTCCTTTA16230.19507961844686333No Hit
CATTAATACTGCTTTGCTTAATGCATCCTGTGCAGCAATGGATGATTTCC15220.18293972845109427No Hit
GCATAAATCTCATAGGCAAATGGTGACAACAACCAATCCATTAATAAGAC14600.17548751875072116No Hit
GCTTATGAGAGAATGTGCAACATTCTCAAAGGGAAATTTCAAACAGCTGC14540.1747663371668141No Hit
CTATGGTATCAAGTACTCTTGAACTGAGAAGCAGGTACTGGGCCATAAGG14380.17284318627639525No Hit
CATATTAAGAGGGTCAGTTGCTCACAAGTCCTGCCTGCCTGCCTGTGTGT14060.16899688449555753No Hit
ATACCATAGTATCCATGTTTTCATTTGAAGCAATTTGTACTCCTCTAGTG13960.1677949151890457No Hit
CCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCATGTCAAAG13650.1640688103388592No Hit
GCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCATTCTGCTGT12550.1508471479672295No Hit
ATACAAGAGAGTAGATGGAAAGTGGATGAGGGAACTTGTCCTTTATGACA11340.13630331935843687No Hit
GATCTATGCTGGGAGTCAGCAATCTGTTCACAGGTTGCGCATATTAGGCC11030.1325772145082503No Hit
CTCTAGCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCATTCT9810.1179131889688065No Hit
ATATAGTGGTATTCCCTCTCCTTCATGACTTGGATCCTCAATGCTTAATT9570.11502846263317819No Hit
GCATAGATCTCATAGGCAAATGGTGACAACAACCAATCCATTAATAAGAC9560.11490826570252702No Hit
GGATAGACCCTTTCAAACTGCTTCAAGCCAGCCAAGTGTACAGCCTAATC9250.11118216085234046No Hit
ACAATAGAGAGAATGGTGCTCTCTGCTTTTGACGAGAGGAGGAATAAATA9240.1110619639216893No Hit
ATCATAATGTGAGTCAAACCAGCCGTTGCATCATCACCATTGTTGGCTTG9150.10998019154582869No Hit
GTCGTACTCCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCA9140.1098599946151775No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAGCTATCTCAATGCATGTGTT8810.1058934959036886No Hit
TGTCAAAGGAGGGCACGATCGGGTTCGCTGCCCTTTCGTCCGAGAGCTCG8740.10505211738913035No Hit
ATCACTCACTGAGTGACATCAAAGTCATGGCGTCCCAAGGCACCAAACGG8680.10433093580522326No Hit
GTGTATGGACCTGCCGTAGCCAGTGGGTACGACTTCGAAAAAGAGGGATA8680.10433093580522326No Hit
GTGCAGACCGTGCCAGAAAGGTGAGATCTTCGATCTCAGCATTTCCTGGG8570.10300876956806031No Hit
GTATTAATGTATACCCCCTTCATTATATATTCTGTGGCTCTGCAATGAGA8530.10252798184545558No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGGGA100.0070861606145.030724
TCGGTCC301.5985734E-596.67552145
AGCGAAA651.6370905E-1189.249671
AAGTACA300.001934924372.50664145
TAGATAT1500.067.6813
GTAGAAA4350.066.680792
CAAGGTA2350.064.7853859
AATAGGG350.003564998562.14855145
CAAGGGT3250.058.0122874
ACATAAT1251.8189894E-1258.0122872
AAGGGTG1800.056.4008375
GGCAGCC400.006048337654.37998145
AGGGTGT1900.053.432376
GGGTAGA1101.6716513E-952.738441
GTACTGA1600.049.854316
AGGGTGG450.00963050848.3435756
ATATTCA1800.048.3435754
TCCCAAC2250.048.343571
ACAAGGG4050.048.343573
TTATATT2250.045.1206672
Produced by FastQC (version 0.11.5)