FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n01_S1_R1_H03-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n01_S1_R1_H03-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1082335
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCATTCTGCTGT29970.27690132907094384No Hit
ATATACAAGAGAGTAGATGGAAAGTGGATGAGGGAACTTGTCCTTTATGA25740.23781915950237217No Hit
ATATACAACAGGATGGGGGCTGTGACCACCGAATCGGCATTTGGCCTAAT21070.1946717051559822No Hit
CTTAATATGAGTGCAGACCGTGCCAGAAAGGTGAGATCTTCGATCTCAGC20380.18829659948167618No Hit
CTCTAGCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCATTCT19970.1845084932114364No Hit
CATTAATACTGCTTTGCTTAATGCATCCTGTGCAGCAATGGATGATTTCC18550.17138871051938634No Hit
CTCATAAGCAATCCTTGTTTTTCTTCCATTCTCACCCCTCCAGAAGTTTC18440.17037238932493176No Hit
GCATAGATCTCATAGGCAAATGGTGACAACAACCAATCCATTAATAAGAC18280.16889410395117962No Hit
CGTTTAAGGAGACGATAAATGCATTTGAAAAAAAGGCGATCAAGAATCCA17780.16427446215820427No Hit
CTCATAGACTCTGGTACTCCTTCCGTAGAAGGCCCTCTTTTCAGACCGCG17370.16048635588796445No Hit
GATCTATGCTGGGAGTCAGCAATCTGTTCACAGGTTGCGCATATTAGGCC16510.1525405720040468No Hit
AATATGGACAGAGCCGTTAAACTGTATAGAAAGCTTAAGAGGGAGATAAC16360.15115467946615418No Hit
CATTATGATCTGGCATTCCAATTTGAATGATACAACTTACCAGAGGACAA15680.14487196662770768No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAGCTATCTCAATGCATGTGTT14620.1350783260265999No Hit
GCATAAATCTCATAGGCAAATGGTGACAACAACCAATCCATTAATAAGAC14390.13295329080183121No Hit
CTTTTCAGACCGCGTTTAAGGAGACGATAAATGCATTTGAAAAAAAGGCG14370.1327685051301122No Hit
GTATAGAAAGCTTAAGAGGGAGATAACATTCCATGGGGCCAAAGAAATAG14290.13202936244323615No Hit
CCCATATACAAGAGAGTAGATGGAAAGTGGATGAGGGAACTTGTCCTTTA13820.1276868991578393No Hit
GCTTATGAGAGAATGTGCAACATTCTCAAAGGGAAATTTCAAACAGCTGC13740.12694775647096324No Hit
CCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCATGTCAAAG13670.1263010066199467No Hit
GTTGTATATGAGGCCCATACAACTGGCAAGTGCACCAGCAGAATAACTGA13540.1250998997537731No Hit
CATATTAAGAGGGTCAGTTGCTCACAAGTCCTGCCTGCCTGCCTGTGTGT13290.1227900788572854No Hit
ATACCATAGTATCCATGTTTTCATTTGAAGCAATTTGTACTCCTCTAGTG13130.1213117934835333No Hit
GTTTTTTACTCTAGCTCTATGCTGACAAAATGACCATCGTCAGCATCCAC12730.117616080049153No Hit
CTATGGTATCAAGTACTCTTGAACTGAGAAGCAGGTACTGGGCCATAAGG12690.11724650870571496No Hit
GTTCTGGCCAGCACTACAGCTAAGGCTATGGAGCAAATGGCTGGATCGAG12540.11586061616782235No Hit
ATATAGTGGTATTCCCTCTCCTTCATGACTTGGATCCTCAATGCTTAATT12210.11281165258445859No Hit
AATATGCGCAACCTGTGAACAGATTGCTGACTCCCAGCATAGATCTCATA11750.10856158213492127No Hit
CAGTTATTCTGCTGGTGCACTTGCCAGTTGTATGGGCCTCATATACAACA11130.10283322631163179No Hit
CGATAAATGCATTTGAAAAAAAGGCGATCAAGAATCCACAATATCAAGTG11040.10200169078889623No Hit
AGTAGAAACAAGGTAGTTTTTTACTCTAGCTCTATGCTGACAAAATGACC11030.10190929795303673No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTAGTG100.0070917057144.994777
AGGACGC259.3814096E-487.01295145
CCCACGC259.3814096E-487.01295145
TCGGTCC259.3814096E-487.01295145
AGCGAAA950.083.9559861
TAGGGGC608.024108E-872.51079145
GTAGAAA7850.069.274621
GTCAATT1100.065.912812
GTGCTCG350.003564480762.152103145
GGGTAGA1400.062.1492351
CTACTAA502.009758E-458.0032736
ATATTCA3400.055.44434
AGGGTGT4350.055.00316
TTAGTAG805.8718797E-754.3780672
TAAATAC805.8718797E-754.3780674
CAAGGGT5850.052.054224
AAGGGTG4600.052.01385
GTACTGA2250.051.5584646
TACTGAT2300.050.4329647
CCAGCTC450.00963368548.340523145
Produced by FastQC (version 0.11.5)