FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n01_S1_R1_H01_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n01_S1_R1_H01_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACAGTTTGGCAGAGGAATGGACCCATCACAGGTCCAGGTCAGTAGCCAA12.631578947368421No Hit
GTGAAACACAGGGAACAGAGAAACTGACAATAACTTACTCATCTTCAATG12.631578947368421No Hit
GCCAGAGGTGATACCATCACTCGGTCTGATCCTGCATCATTCATTTTACT12.631578947368421No Hit
CACTATTTGTTTATTGTTCTAAATACATTCTAATATGTATCCGCTCATGA12.631578947368421No Hit
CTTTTAAAGTTCTGCTATGTGCCGCGGTATTATCCCGTATTGAATCCTGG12.631578947368421No Hit
AATAACCCGGCGGCCCAAACTGCCGACTCGGAGCGAAAGATATACCTCCC12.631578947368421No Hit
GTAATCCAGCATCGTTTGTTTTGCTCCAGAGGGTTTGTCCTTGTTCATTA12.631578947368421No Hit
CCTGGAGTATACATCTGTTCCCAGCATGTTCCTTGAGTCAAATGCACCAC12.631578947368421No Hit
GAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAACCGCCTGGTATCTT12.631578947368421No Hit
TCTGGCAATATCCCAATCATTCCCATCACATTGTCGATAGGTTCAACTCC12.631578947368421No Hit
AAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAA12.631578947368421No Hit
ACTCTGAACTTTATGGAAATAATCATTTAGTAAACAAGACTATGGCTTGA12.631578947368421No Hit
CAGCAGTGTGTACATTGAAGTGTTGCATTTGACTCAAGCACCATGCTGTG12.631578947368421No Hit
GATCTAGGTGAAGATCCTTTTTGATAATCTCATGACCAAAATCCCTTAAC12.631578947368421No Hit
ATACAATAAAATGGAATATGAAACACATCAGTATGTATAAACTAAGGCCA12.631578947368421No Hit
GGTGATGGGCATGATTTTTGCCGTGTGAGGTGTGCAGTGGTGGACGGGAT12.631578947368421No Hit
CCTCTCATTGACATCTCGATGCTTGGAGTCATGTATGGCAATATCACAAT12.631578947368421No Hit
CCATAAGAGAAGATGGACAGCTATGACTGGGAGTAGTCAGGAGAGGAGGA12.631578947368421No Hit
TGTTATAGTTGAATACAGGAGAATTGCCCCTTACAAGTATTCTCATTCCT12.631578947368421No Hit
GGCTGTGACATGGTGGAATAGGAATGGACCAATAACAAATACAGTTCATT12.631578947368421No Hit
GCACCCATAATGTTCTCAAACAAAATGGCAAGACTAGGGAAAGGGTACAT12.631578947368421No Hit
CCCATCAAGTTGTGGAGACGCATATAGACTGTTGAATACAGATTTTGCCA12.631578947368421No Hit
CCGCATTTACTGTGAAGGGGAGGGGATCAGGAATGAGAATACGTGTAAGG12.631578947368421No Hit
GACATGCCTCCAAGCATATAGATGTAAATGAGAGGAGTGAGCATCAGCAA12.631578947368421No Hit
GTGCCCCACTGATGCCCAATATTAATAAGTGTGGAGACGGGGTGAACGTG12.631578947368421No Hit
CTTAGGATGAAATGGATGATGGCAATGAAATATCCAATTACAGCAGACAA12.631578947368421No Hit
ATTAATAACTAGTCAATAATCAATGTCGACCCAGGTGCCACATTTCCCCC12.631578947368421No Hit
GTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTAATGC12.631578947368421No Hit
GCTCGGTACCCGGGGATCCTCTAGACCGCAGTACTGGTCGACCTCCGAAG12.631578947368421No Hit
GTTTAAAAACGACCTTGTTTCTACTAATAACCCGGCGGCCCAAAATGCCG12.631578947368421No Hit
CGCCACTAATAAGAAAGCGCAAAGTTGAAGACAAACAAAAAGGCATCGCC12.631578947368421No Hit
TCTATGGTGTTGGCCAATGCAGTTGCTGCCGGTTGATTTCTGTTTAATGT12.631578947368421No Hit
ACTGTCTATTTCGGTACTACAAACTTGCAGCCCTGCCACTCGAGGACAAA12.631578947368421No Hit
GGGTGGGGGGATTTGGCGGGGAGACATCCTAAGGCAGACCCCCACAGACG12.631578947368421No Hit
GTACATTGAAGTGTTGCATTTGACTCAAGCAACATGCTGGGACCACATGT12.631578947368421No Hit
GGTACATCTGTTCATCCTCAAGAATTCCCCTTTGGCTTGTGTTTAGAATA12.631578947368421No Hit
GCTGGGAAAAGATGTATACTCCAGGCTGGGAAGTGAGGAATTATGATGTT12.631578947368421No Hit
CTATTAATAACAAGTCAATAATCAATGTCGACCCAGGTGGCACTTTTCGG12.631578947368421No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)