FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n01_S1_R1_G11_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n01_S1_R1_G11_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1029445
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCATTCTGCTGT12650.12288174696074099No Hit
ATATACAAGAGAGTAGATGGAAAGTGGATGAGGGAACTTGTCCTTTATGA11500.11171067905521906No Hit
ATATAAATCCTGGCCATGCAGACCTCAGTGCCAAGGAGGCACAGGATGTA11040.1072422518930103No Hit
CATTAATACTGCTTTGCTTAATGCATCCTGTGCAGCAATGGATGATTTCC10820.10510517803282352No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA1600.099.730671
TACGCGT301.5960464E-596.708536
ATACGCG353.4299508E-582.893025
GTATACG553.2121735E-452.7501033
ATATTCA3550.038.819624
CGCGTGA750.001483792838.664628
GGTCGTT1553.472087E-837.4173747
GTATTAC3900.035.3358081
GTAGAAA8200.034.4966352
CGTACTC2800.033.675293
TCGTACT2800.033.675292
ACGCGTG900.003629221332.2205167
CAAGGTA4000.030.810879
TTAATAC7450.029.2072723
TTATATT5550.028.7511832
TATTACA4800.028.7103442
GTACTGA2303.4733603E-828.381856
TAATGTA4100.028.3049325
TAGCTCT7600.027.6764534
ATATAGT6150.027.1255631
Produced by FastQC (version 0.11.5)