FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n01_S1_R1_G07-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n01_S1_R1_G07-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences938963
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATATATACTTACACAGTTTGAGCATTCACAGGCCACTAGAAGCCCATCT15890.16922924545482623No Hit
TGTATATACAGCTCGCTGAAGAGGTCCATGACCTCAAAAAAGATCAAATG14690.15644918915867823No Hit
GTATATACATACATATATACTTACACAGTTTGAGCATTCACAGGCCACTA12780.13610759955397606No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACAG10050.038.2413445
CGAAAGC3450.033.6297952
AGCGAAA2850.033.0766751
CAGCTCG11400.031.1617669
TATATAC21950.031.0539383
CATATAT11150.029.9161911
ATACTTA11250.029.00576
ATATACT11850.028.148994
GTATTAG3900.027.8900951
TATACTT12200.027.341445
GCGAAAG4350.026.6719071
TACTTAC11800.025.8046597
ATATACA17000.025.5932664
ATACAGC15250.025.2016746
TATATAT9100.024.7026542
ATATATA13650.024.4370372
ACTTACA12200.024.3643538
TGTATAT15300.024.1714151
GTATTAA6550.023.2488421
CTTACAC11900.023.1508799
Produced by FastQC (version 0.11.5)