FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n01_S1_R1_G06-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n01_S1_R1_G06-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences964755
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTATATACAGCTCGCTGAAGAGGTCCATGACCTCAAAAAAGATCAAATG12870.13340174448435096No Hit
CATATATACTTACACAGTTTGAGCATTCACAGGCCACTAGAAGCCCATCT12750.13215790537493977No Hit
GTATATACATACATATATACTTACACAGTTTGAGCATTCACAGGCCACTA12030.12469487071847256No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAGCG350.003563092862.15775
AGCGAAA2200.046.152171
GTACTAC1501.1308039E-633.844921
CGAAAGC3600.030.2155483
ACCGGTA1255.155886E-428.9979049
ATATACT8950.025.9279794
GTATATA15000.025.625441
CAGCTCG7100.025.5263279
ATACTTA9850.024.2951416
TATATAC17950.024.2397733
GCGTGCC1851.831497E-423.5118189
TATACAG8350.023.4487115
ATATATG8200.022.9932963
CACCGGT1902.1941637E-422.8930848
TAGCGTT1600.002172078222.6616615
GTTACGC2903.2782555E-722.5053733
GCGAAAG4900.022.2003292
TTACGCA3304.677713E-821.9749434
CATATAT10900.021.9570621
TAGAAAC8750.021.5480023
Produced by FastQC (version 0.11.5)