FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n01_S1_R1_G05_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n01_S1_R1_G05_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGTAGTAGTTAATTCTTCCTTCTTGACCTCTTACTTTGGGTCTTTTTGC15.555555555555555No Hit
TTTTCATGAAGGACAAGTTAAATTCATTATTTTTGCCGTCTGAGTTCTTC15.555555555555555No Hit
CAACACTTCAATATAAACACTGCCTGTTACGACGGCTACTGGGAGAAACC15.555555555555555No Hit
CCCCCACCACTCGTGGGTGGGCCAAATGAGCATTCTTTAGGATTCGACCA15.555555555555555No Hit
GTTAAATACATATTCTACACTGTAGAGACCCATTAGAGCCCATCCAGAAA15.555555555555555No Hit
GGGGAGGGAATACCACTATATGATGCAATCAAATGCATGAAGACATTCTT15.555555555555555No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA15.555555555555555No Hit
GTAGAATCTGGTGACCCGAATGCACTATTGAAGCACCGATTTGAGATAAT15.555555555555555No Hit
AGGGAGTGTACATAAATACGGCCTTGCTCAATGCATCCTGTGCAGCCATG15.555555555555555No Hit
GGGTGGGAAAGTGTGCAGGACCTTACTGGCAAAATCTGTATTCAAACGTC15.555555555555555No Hit
GGCCATGTTTCCGATTTGTTTTCAAAGAATTCCTTGGTCATGTATTTCTC15.555555555555555No Hit
AATATATTCAATATGGAGAGAATAAAACAACTGACAGATCTAATGACGCA15.555555555555555No Hit
GGTTTTTACTCTAGCTCTATGTTGACAAAATGACCATCGTCAACATCCAC15.555555555555555No Hit
TACATATACTGTTCACCACTGTCAAGGCCATGATTCGGTATCTTCCTTCA15.555555555555555No Hit
AACTACTTCAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAG15.555555555555555No Hit
GACAAAGCCAGAGGGACACACTCCCCCCTTCAGGGGGCACTCATGGACCC15.555555555555555No Hit
GTTTAAGACACATTCTGTGTATATGTGCGTGAATCTGTCATTCTTCAGAT15.555555555555555No Hit
GTGTAGTTGCAACGGCATCATATTCCATGCTTTTGGCTGGACCATTGGCT15.555555555555555No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)