FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n01_S1_R1_G05-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n01_S1_R1_G05-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences975116
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA1800.092.654811
CTACGAG450.00964033148.3317269
GTGTACG1109.9455065E-846.144291
ATACGTT700.001056267841.4314426
ACGTTGA800.002036922136.2487958
GTAGGTA2850.033.0690778
TAGACCG950.00472563630.528435
GGTCGAC1204.0551068E-430.211975145
GTATAAA8050.028.824831
ATATACT8650.026.822674
TATATAC12100.025.7662493
CTTACAC5150.025.3389639
TTAATAC6950.025.0377063
CAGTTCG1450.001225559625.003014145
TATAAGG4400.024.7176232
TAAAGTA5300.024.624354
TCTATGC10600.023.940343
GTATATA10300.023.9361521
TATAGGC3102.4159817E-823.3887182
TATAGAC4700.023.13992
Produced by FastQC (version 0.11.5)