FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n01_S1_R1_G04_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n01_S1_R1_G04_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1090649
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATTAGAAGAGGCCTTATTTTCTCAATTTTCTTCTTTGTTGATTCATTG37140.34053118831081314No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT25760.23618964488116712No Hit
CTCTTATTCAGTCTTTGTTTTTTCTTCCCTATTGTTCTTTGCGTGACCAT25650.23518107108703168No Hit
AAATAAGACAGGGACATTTGAATTCACAAGCTTTTTTTATCGCTATGGAT24670.2261955954665525No Hit
TCCTTAGACAGAATCCAACTGAGGAACAAGCCGTAGACATATGCAAGGCA23730.21757687395303166No Hit
GTATATTGTCTTGGTGTATTTCTTTTGTCCAAGATTCAGTATCGAGACTC23450.2150095952043233No Hit
GTATAAGTTTGGTCCTCCATCTGATACTAATAGCCCTACCTTTGATTGGG22450.20584074253036494No Hit
GAATAAGAGAGGCTATCTAATAAGAGCACTGACATTAAATACGATGACCA22080.20244826704100038No Hit
CTTTATGACTGACAAAGGGATTCAGGGGATTACAAAGTCTTCCCCGATAA21890.20070618503294826No Hit
GTATAACATTGTGGGATCTTGTGACCATTGAATTTTCACAATTTCCCAGT19870.1821851026315524No Hit
TTATAGGTGCCATGCTCAGGATGTTTCTGAACCACTCGGGTTGATTTCTG19430.17815080745501075No Hit
GTCTAAGGATGTCCACCATCCTTACTCCTCCAATCTGTGTGCTGTGGCAC19360.17750898776783366No Hit
AACTTATACAATATACGGAATCTTCACATTCCTGAAGTCTGCTTAAAATG19260.1765921025004378No Hit
ACACTAAGTGGAATGAAAATCAAAATCCTCGAATGTTCCTGGCGATGATT17260.1582543971525211No Hit
TCCCAGGACTCAGTGATGCTGTGCCATCTATTAGAAGAGGCCTTATTTTC16940.15532036429685445No Hit
ATATAAACACTGCCTGTTCCGCCGGCTACTGGGAGAAACCTTGTTTTACG16480.1511026920668336No Hit
CCCATAATGTTCTCAAACAAAATGGCAAGACTAGGGAAAGGGTACATGTT16380.15018580679943777No Hit
CTTCTAATAGATGGCACAGCATCACTGAGTCCTGGGATGATGATGGGCAT16240.1489021674250836No Hit
GGCTAATAGATTTCTTAAAGGATGTAATGGAATCAATGAACAAAGAGGAA15910.14587644604267735No Hit
GTACTATTGTCTCCCGAAGAAGTCAGTGAAACGCAAGGAACTGAGAAGTT15760.1445011181415836No Hit
CATATAGGTGCCATAGGGGAGACACACAAATTCAGACGAGAAGATCATTT15670.14367592140092733No Hit
TATTTAATGTCAGTGCTCTTATTAGATAGCCTCTCTTATTCAGTCTTTGT15580.14285072466027107No Hit
TTCGTATACTTTGTTGAAACTTTAGCTAGGAGCATTTGCGAAAAGCTTGA14630.13414031462001066No Hit
GATTAGAGGTTTCGTATACTTTGTTGAAACTTTAGCTAGGAGCATTTGCG14620.13404862609327106No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA13810.1266218554273648No Hit
GTATACGAAACCTCTAATCTGCATCCCAGGTGTTGCGATAGCCCTTCTTT13740.1259800357401877No Hit
ATGTTATACAACAAAATGGAATTTGAACCATTTCAGTCTCTTGTCCCTAA13700.1256132816332294No Hit
ATATAGTGGTATTCCCTCCCCCTCGTGACTCGGGTCTTCAATACTTAATT13700.1256132816332294No Hit
TTCCTGAAGTCTGCTTAAAATGGGAGCTAATGGATGATGATTATCGGGGA13570.1244213307856148No Hit
CCATAGAAGTCTTTAGATCGAATGGCCTAACAGCTAATGAGTCAGGAAGG13120.12029534708233355No Hit
ATATAAATAAGACAGGGACATTTGAATTCACAAGCTTTTTTTATCGCTAT13050.11965352739515646No Hit
CACATATAGGTGCCATAGGGGAGACACACAAATTCAGACGAGAAGATCAT12840.11772806833362522No Hit
CTAATAGCCCTACCTTTGATTGGGTTTGATCCCACAGCTTCTTTAACTCA12650.11598598632557312No Hit
CATTAAATACGATGACCAAAGATGCAGAGAGAGGCAAGTTAAAAAGAAGG12640.11589429779883353No Hit
CCTATATGTGTATCTGTAGTCTTTGATGAACAATTGAAGAGCCATCTGGG12610.11561923221861478No Hit
ACTTAGTGTTGTCCCCAGTGATTGTGAAAGAAATCTCTGTGTCTTGTGAA12460.11424390431752104No Hit
CCACTATATGATGCAATCAAATGCATGAAGACATTCTTTGGCTGGAAAGA12440.11406052726404188No Hit
ATATAAAACTTATTTCGAAAAGGTCGAAAGGTTGAAACATGGTACCTTCG12190.11176831409555228No Hit
GTTCTAGAGGCTATGGCTTTCCTTGAAGAATCCCACCCAGGAATATTTGA12190.11176831409555228No Hit
ATCTGATACTAATAGCCCTACCTTTGATTGGGTTTGATCCCACAGCTTCT12170.11158493704207312No Hit
TATTAGAAGAGGCCTTATTTTCTCAATTTTCTTCTTTGTTGATTCATTGA11850.10865090418640644No Hit
CATATAGACTGTTGAATACAGATTTTGCCAGTAAGGTCCTGCACACTTTC11640.10672544512487518No Hit
CTTCAGGAATGTGAAGATTCCGTATATTGTATAAGTTTGGTCCTCCATCT11590.10626700249117726No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG11400.10452492048312519No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT11360.10415816637616684No Hit
GAACTGGGGAATTGAATCCATCGACAATGTGATGGGAATGATCGGAATAC11340.1039747893226877No Hit
GGATAAGAGTCAGCAAAATGGGAGTAGATGAATACTCCAGCACGGAGAGA11330.1038831007959481No Hit
CTTATTAGATAGCCTCTCTTATTCAGTCTTTGTTTTTTCTTCCCTATTGT11310.10369972374246893No Hit
GTCTGGGCTCCCAGTAGGGGGCAATGAAAAGAAGGCCAAACTGGCAAATG11190.10259946142159393No Hit
CCTATAATGTTCTCAAACAAAATGGCAAGACTAGGGAAAGGGTACATGTT11090.1016825761541981No Hit
GTCCTATATAAATAAGACAGGGACATTTGAATTCACAAGCTTTTTTTATC11040.10122413352050019No Hit
ATTATTGCCTCAGCAATTGACTGCTCGTCTCTCCCGCTTACTATCAACTG11030.10113244499376059No Hit
GTATTGGAGTAACAGTGATAAAGAACAACATGATAAACAATGACCTTGGA10960.10049062530658351No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGAGG100.0070875445145.02333
CGAGGCA100.0070875445145.02335
CCGAGGC100.0070875445145.02334
GAGTTAT100.0070875445145.0233145
AGGGTGA301.598249E-596.68226
CAGGTAC1300.094.822923
AGCGAAA554.1654857E-1092.287551
GCAGGGT406.1119863E-790.639564
GCAGGTA1400.088.049862
AGTAGAA3300.083.498261
GACAGTA1750.082.8552557
AGGTACT1650.079.1036154
AGTCGCG300.001934556272.51165145
GGTACTG1700.072.511645
GCGAAAG754.8494257E-967.6775362
GTAGAAA4950.065.919671
CAGGGTG451.1929579E-464.4547965
TACAACG451.1940412E-464.442988
GCCCACC902.0470907E-856.397945145
CGAAAGC902.0470907E-856.3979453
Produced by FastQC (version 0.11.5)