FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n01_S1_R1_G04-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n01_S1_R1_G04-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1047871
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCATTCTGCTGT12950.12358391443221542No Hit
ATATACAAGAGAGTAGATGGAAAGTGGATGAGGGAACTTGTCCTTTATGA11580.11050978603282274No Hit
ATATAAATCCTGGCCATGCAGACCTCAGTGCCAAGGAGGCACAGGATGTA11210.10697881704904515No Hit
CATTAATACTGCTTTGCTTAATGCATCCTGTGCAGCAATGGATGATTTCC11010.10507018516592215No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA1350.091.313221
TATACGT450.00964311148.3284537
CCGTAGT951.9185227E-645.797961
ATATTCA4400.042.84684
CTATACG1252.715151E-740.6075251
TACGCAC1252.7161695E-740.6055874
GGTCGTT907.487143E-540.273717
TATACGC1553.466448E-837.4245032
GTATACC3800.034.3386349
ATACGCA1501.1327411E-633.837993
CAACGTA900.003630105232.2189679
GTAGAAA7950.031.9241541
TTATATT5950.031.6850322
GTATTAC3100.030.408861
TAATGTA4550.030.278895
GGATAGC3850.030.1340145
TAGTGCA1453.4872755E-530.004134
TATAGAC3201.8189894E-1229.4571782
TACCTCG200.006070993429.005653140-144
GTATAAT3001.8189894E-1129.0053751
Produced by FastQC (version 0.11.5)