FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n01_S1_R1_G01_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n01_S1_R1_G01_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATAGGAGAGTAAGAGACAACATGACCAAGAAGATGGTCACGCAAAGAAAA13.571428571428571No Hit
AAAAAATGGAGTGTTTCTTGACCCAAAAGCTACATTAATTATTTTGACAG13.571428571428571No Hit
CACTGAGGATGGAGCAATAGTTGGAGAAATTTCACCATTAACTTCTCTTC13.571428571428571No Hit
GGTAATGGTGAAATTTCTCCAACTATTGCTCACTCCTCAGTGAAAGCCCT13.571428571428571No Hit
GAAATCAACCCGAGTGGTTCAGAAACATCCTGAGCATGGCACCCATAATG13.571428571428571No Hit
GTGAAAATTCAATCGTCACAAGATCCCACAATGTTATAAAACAAAATCGA13.571428571428571No Hit
GGGCAAGCTTTCCCAAATGTCAAAAGAAGTGAACGCCAAAATTGAACCAG13.571428571428571No Hit
GTACATGAAGGGTATGAAGAATTCACAATGGTTGGGAGAAAAGCAACAGC13.571428571428571No Hit
GGCGGAGACAGTGGTGTGGTGTAAATGGACGAAGTCACAGGGTGGCATAG13.571428571428571No Hit
GCAGACGGATATTCGGAGCCAATCAACACCATAATTATAATACAGGAATT13.571428571428571No Hit
GTTCAAGAGTGATTGTATACATGCTGAGTTCTGGAATGTTTGACAATCAC13.571428571428571No Hit
GGTTTATATAACTGATGGTAATGAACTGTACGTGTTGTTGTGCCATTCAT13.571428571428571No Hit
ATAACAGCCAGACAAAAACATAATGTCATCAAACAAGATGTCAAGAATAC13.571428571428571No Hit
AGCGAAAGCAGGGAGGCCTCCTGAACTCATAAAGTTAATGAGAAATAAGA13.571428571428571No Hit
TCTATGCCCAAAATGAGAAATCCTCTCAGGACAGAAGACTACCCCCCAGA13.571428571428571No Hit
ATGTTATACACCAAAATGGAATTTGAACAATTTCAGTCGCTTGTCCCTAA13.571428571428571No Hit
GTATAAACTATGTCTGTTACAGTTTGGAATAGGAATGGACAAATAAAAAC13.571428571428571No Hit
GCGGCACCCCGTAAGCGCTGTTGAGCCACTCCCAACCCGATATGGTCGGC13.571428571428571No Hit
GATATTGCACATTCTTTCATAAGCAACCCTTGTCCTTCGTCCATTTTCAC13.571428571428571No Hit
TTAAATAAGCTGAAACGAGAAAGCTCTTATCTCTAGTTCTACTTAAAGCA13.571428571428571No Hit
ATGAAGATGGATTCGAGCCCAACGGCTGCATTGAGGGCAAGCTATCCCAA13.571428571428571No Hit
AGGCAATACTAGTAGTTCTGCTATATACATTTGCAACCGCAAATGCAGAC13.571428571428571No Hit
GCTCTGGAGGTAGTGAAGGTCTCCCATTCTCATCACAGTTTCTCCACGCG13.571428571428571No Hit
GATGTTAACAAATGTCTGATTTACCCAACAGTACTTTTCATAAGAAATGA13.571428571428571No Hit
AATATATGTAATTCATCGAAAAATCACCCGAAAACCAACAGTTAGGCAAT13.571428571428571No Hit
CCCATAATGACAAAGATCATATCTACTCTAATTGACTACATCAACAAATC13.571428571428571No Hit
CTCCCATTCTCATCACAGTTTCTCCAAGCGAATCTCTGTATATTTTCAGA13.571428571428571No Hit
AACTTATACAATATACGGAATCTTCACAGTCCTGAAGTCTGCTTAAAATG13.571428571428571No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)