FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n01_S1_R1_G01-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n01_S1_R1_G01-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences869058
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG83070.9558625546281146No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA75750.8716334237760887No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA68720.7907412393649215No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA66470.7648511376686021No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT58160.6692303620701955No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG53550.6161844203724032No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT52050.5989243525748569No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC47850.5505961627417273No Hit
GGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATG47630.5480646861314205No Hit
GTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCC46160.5311498196898251No Hit
ATGCTGGGCCATATCTCTTGTCTTCTTTGCCCAAAATGAGAAATCCTCTC44650.5137746847736284No Hit
GGATGATGGCAATGGTAAGAGGCAATTCTCCAGTATTCAATTACAACAAG43710.5029583756204994No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG39720.45704659527902625No Hit
ATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCC38790.4463453532445476No Hit
ATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTG37720.43403317154896454No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT35980.4140114929038108No Hit
CCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACC34900.40158424408957744No Hit
AATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGT34790.4003185057844241No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTG34290.3945651498519086No Hit
AAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCCGAAT33480.3852447132412336No Hit
TTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCT29620.34082880544221444No Hit
GTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGATC27700.3187359186613552No Hit
CAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCA27480.31620444205104836No Hit
CATTTGGAAGTTTGTTTCATGTATTCGGATTTCCATTTCATCGACGAACG26990.31056615323718323No Hit
GTTCATTGATGCTTAATGCTGGGCCATATCTCTTGTCTTCTTTGCCCAAA26200.30147585086380885No Hit
ACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGA26160.3010155823892076No Hit
AGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATC25520.2936512867955879No Hit
GATCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGACTCGA24140.2777720244218453No Hit
ATGCATATCTCTTGTCTTCTTTGCCCAAAATGAGAAATCCTCTCAGGACA23950.2755857491674894No Hit
ATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACACCATG23730.2730542725571826No Hit
TAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATACTC23550.270983064421477No Hit
CTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAG21430.2465888352676116No Hit
CTTCAATGGTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTG21100.24279162035215138No Hit
CCACCATGCTAGAAATTCCAACCGGTCTCCTATATGAACTGCTAGGGAAA20590.23692319730098566No Hit
CTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGA19930.22932876747006528No Hit
CACTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTTGCAA19910.2290986332327647No Hit
ATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACT19870.22863836475816346No Hit
GTCTATATGCGTCTCCACAACTTGAGGGGTTTTCGGCTGAATCTAGAAAA19740.22714249221570942No Hit
GTCCCGCACTCGCGAGATACTCACTAAGACCACTGTGGACCATATGGCCA19710.22679729085975847No Hit
AAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTTTGCCG19460.22392061289350076No Hit
CTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTTGCAAGA19280.22184940475779522No Hit
CCATTTGAATGGATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCG18990.21851245831693628No Hit
CCATACAGCCATGGAACAGGAACAGGATACACCATGGACACAGTAAACAG18990.21851245831693628No Hit
GTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCTAGACACCATG18900.2174768542490835No Hit
CTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAA18550.21344950509632268No Hit
AATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCAC18370.2113782969606171No Hit
ATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAAT18090.2081564176384085No Hit
CATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGATGCTTAAT17810.20493453831619984No Hit
CACCAATACTCAGAAAAGGGAAAGTGGACGACAAACACAGAGACTGGTGC17630.20286333018049427No Hit
GGATACACCATGGACACAGTAAACAGAACACACCAATACTCAGAAAAGGG17200.197915444078531No Hit
ATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTC17180.19768530984123037No Hit
CTCCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTT16890.19434836340037143No Hit
AATTAGCACATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGA16780.19308262509521804No Hit
ATACAGTTCTTGGAAAGGATGCAGGTGCATTGACTGAAGATCCAGATGAA16780.19308262509521804No Hit
CATATAGACTGTTGAATACAGATTTTGCCAGTAAATCGGTGCTTCAATAG16470.18951554441705848No Hit
ATACTCACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACAC16410.1888251417051566No Hit
ACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG16060.1847977925523958No Hit
GATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGT15700.1806553762809847No Hit
ATGGAAGACTTTGTGCGACAATGCTTCAATCCAATGATCGTCGAGCTTGC15420.17743349695877605No Hit
GCTTAATGCTGGGCCATATCTCTTGTCTTCTTTGCCCAAAATGAGAAATC15180.17467188611116866No Hit
TTACCAACACTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCT14870.17110480543300907No Hit
CAGTAAACAGAACACACCAATACTCAGAAAAGGGAAAGTGGACGACAAAC14830.17064453695840784No Hit
CATATAGACTGTTGAATACAGATTTTGCCAGTAAGGTCCTGCACACTTTC14770.169954134246506No Hit
ATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTA14700.16914866441595383No Hit
GCGGAAAAGGCAATGAAAGAATATGGGGAAGATCCGAAAATCGAAACTAA14340.1650062481445427No Hit
GTCCCTAAGTGCCTGAACAATGAGAAGCAATTTTCTAGATTCAGCCGAAA14110.16235970441558562No Hit
GTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTTT14030.16143916746638315No Hit
CTACCTTTCCTAAAAATTCCAGCGCAAAATGCCATAAGCACCACATTCCC14030.16143916746638315No Hit
ATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACCCC13740.15810222102552418No Hit
TCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTATGCTAGAGTCC13740.15810222102552418No Hit
CTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGG13740.15810222102552418No Hit
GCTTCATATAGCCCCCCAAGATCGAAGGTTCCAGGTTCCAGGTTGTCCCT13550.15591594577116832No Hit
GACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGC13530.1556858115338677No Hit
ATATGGGGAAGATCCGAAAATCGAAACTAACAAGTTTGCTGCAATATGCA13470.15499540882196586No Hit
GTACACATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAATGAAGTGGA13200.15188859661840753No Hit
CCTCCATACAGCCATGGAACAGGAACAGGATACACCATGGACACAGTAAA13170.15154339526245658No Hit
CATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAATGAAGTGGATGATG12980.14935712000810072No Hit
GGATGATACAGTTCTTGGAAAGGATGCAGGTGCATTGACTGAAGATCCAG12740.14659550916049333No Hit
AAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTTTCCTA12040.1385408108549717No Hit
GATATGCATTAAGCATCAATGAACTGAGCAATCTTGCAAAAGGAGAGAAG12030.1384257437363214No Hit
GAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAA11910.13704493831251768No Hit
CTACTTTTCCTAAAAATTCCAGCGCAAAATGCCATAAGCACCACATTCCC11870.13658466983791645No Hit
CCACATTCCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACA11810.1358942671260146No Hit
GAATATGGGGAAGATCCGAAAATCGAAACTAACAAGTTTGCTGCAATATG11770.13543399865141337No Hit
CCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTTGCAAGATTGCTCA11570.1331326562784072No Hit
AGTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGAT11530.13267238780380594No Hit
TCATATAGGAGACCGGTTGGAATTTCTAGCATGGTGGAGGCCATGGTGTC11460.1318669179732538No Hit
GCATTAAGCAAAACCCAGGGATCATTAATCAGGCACTCCTCGATTGCTTC11380.13094638102405132No Hit
TCTCAGGACAGCAGACTCCACCCCAGATGTGCCTTCATCTGGATCTTCAG11320.13025597831214947No Hit
GGACAAGTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAA11280.12979570983754823No Hit
CCCCAGATGTGCCTTCATCTGGATCTTCAGTCAATGCACCTGCATCCTTT11040.12703409898994084No Hit
GATCTTATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGACTCGAA10990.1264587633966893No Hit
GCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTGAGGAAGGAG10950.12599849492208806No Hit
GCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACCCCGCACT10940.12588342780343775No Hit
GTATCATCCACTTCATTCTGAGTGCGGGGTTCTTCTCTTGCCTTCCTGAT10940.12588342780343775No Hit
TCAATGAACTGAGCAATCTTGCAAAAGGAGAGAAGGCTAATGTGCTAATT10750.12369715254908187No Hit
CATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAATGAAGTGGATGATA10610.12208621288797757No Hit
AACTACTTCAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAG10430.120015004752272No Hit
GAATGAAGTGGATGATACAGTTCTTGGAAAGGATGCAGGTGCATTGACTG10400.11966980339632106No Hit
GTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAA10300.11851913220981798No Hit
CGCGAGATACTCACTAAGACCACTGTGGACCATATGGCCATAATCAAAAA10080.1159876555995112No Hit
AGTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCAT9990.11495205153165841No Hit
GGCAATGAAAGAATATGGGGAAGATCCGAAAATCGAAACTAACAAGTTTG9970.1147219172943578No Hit
TCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTGGACCATAT9780.11253564204000194No Hit
TACCAACACTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTT9620.11069456814159699No Hit
CTCCTCGATTGCTTCATATAGCCCCCCAAGATCGAAGGTTCCAGGTTCCA9560.11000416542969514No Hit
GAACAGGAACAGGATACACCATGGACACAGTAAACAGAACACACCAATAC9430.10850829288724113No Hit
TATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCTGG9370.10781789017533928No Hit
TCATTAATCAGGCACTCCTCGATTGCTTCATATAGCCCCCCAAGATCGAA9340.10747268881938835No Hit
GGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCG9280.1067822861074865No Hit
CTTCTGGTACATCTGTTCATCCTCAAGAATTCCCCTTTGGCTTGTGTTGA9260.10655215187018588No Hit
GTTCATTGATGCTTAATGCATATCTCTTGTCTTCTTTGCCCAAAATGAGA9120.10494121220908155No Hit
GGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGA9120.10494121220908155No Hit
ATATAGCCCCCCAAGATCGAAGGTTCCAGGTTCCAGGTTGTCCCTAAGTG9040.1040206752598791No Hit
GGATGATGGCAATGAGATACCCAATTACAGCAGACAAGAGAATAATGGAC8930.10275493695472569No Hit
GATATGGCCCAGCATTAAGCATCAATGAACTGAGCAATCTTGCAAAAGGA8910.10252480271742508No Hit
GGCCTCCACCATGCTAGAAATTCCAACCGGTCTCCTATATGAACTGCTAG8880.10217960136147414No Hit
GCCATAAGCACCACATTCCCTTATACTGGAGATCCTCCATACAGCCATGG8870.10206453424282384No Hit
GGATACACCATGGACACAGTAAACAGAACACACCAATACAGATGTACCAG8850.10183440000552323No Hit
GAATAGAACGATTCCTCTTGGGAATCCAGGAATGTGTAGTTGCAACGGCA8840.10171933288687292No Hit
ATCCAATGATCGTCGAGCTTGCGGGAAAGGCAATGAAAGAATATGGGGAA8830.1016042657682226No Hit
GTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCCAATGA8740.10056866170036984No Hit
GCCATGGAACAGGAACAGGATACACCATGGACACAGTAAACAGAACACAC8710.1002234603444189No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGTGA100.007086364145.02976
GCAGGTA5950.0116.998752
CCTAGCG256.4652104E-6115.9970559
AGTAGAA12900.0109.628091
ACCGGAG203.8627372E-4108.772273
CCGGAGA203.8627372E-4108.772274
TACCGGA203.8627372E-4108.772272
CCGCACG404.811227E-9108.75975145
CAGGTAC6450.0106.804813
GGTACTG6600.0104.377435
CCTCTGC301.5968022E-596.69761
ACAGACG301.5986287E-596.67534145
AGCGAAA1800.092.6685261
CAAACGC406.1135324E-790.633125145
GGTCAAA8000.088.840911
ACTGATC8000.086.9977958
AGCAGGC2650.084.828693
GTCAAAT8400.084.6006552
CGAAAGC2050.081.358123
GCGAAAG2050.081.358122
Produced by FastQC (version 0.11.5)