FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n01_S1_R1_F12-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n01_S1_R1_F12-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13507
Sequences flagged as poor quality0
Sequence length151
%GC51

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTAAGGCAGGACGTCACCGAGTGAGGACCGCTCTCTGGCTGGCTGCC1821.3474494706448508No Hit
GGTCTAGACAGGACCCTGCTCTAAACTGGGGGCCCACAACTGGAAGCATG1421.0513067298437846No Hit
TCCCACGCCTGTCCTGTCCACTGTTCAGTTACATTTTGGGGGGAAATCCA1310.9698674761234914No Hit
ATCTATAACAGGGGATCTGCCTGGGGATAGAAGCCAGGCCTACAATGGGG970.7181461464425853No Hit
GCTCTAAACTGGGGGCCCACAACTGGAAGCATGTTGTCCCCCAACAATGA730.5404605019619457No Hit
CGCCTGTCCTGTCCACTGTTCAGTTACATTTTGGGGGGAAATCCAATCAG670.49603909084178577No Hit
GTTCAGTTACATTTTGGGGGGAAATCCAATCAGGGCACACCTCTAAGGCA590.4368105426815725No Hit
GATCTATAACAGGGGATCTGCCTGGGGATAGAAGCCAGGCCTACAATGGG550.4071962686014659No Hit
GTCCTGTCCACTGTTCAGTTACATTTTGGGGGGAAATCCAATCAGGGCAC540.3997927000814393No Hit
CCTGTCCACTGTTCAGTTACATTTTGGGGGGAAATCCAATCAGGGCACAC510.37758199452135927No Hit
GTTACATTTTGGGGGGAAATCCAATCAGGGCACACCTCTAAGGCAGGACG500.37017842600133266No Hit
GTCCATAGCACTGGGGCTTGAGTTGCATCTGCCCTGCATCTGGGAGGTCG470.3479677204412527No Hit
AGACAGGACCCTGCTCTAAACTGGGGGCCCACAACTGGAAGCATGTTGTC470.3479677204412527No Hit
GGCCTACAATGGGGGATCAGGTCTAGACAGGACCCTGCTCTAAACTGGGG450.33316058340119936No Hit
GTGATGGGGGATCTGCACGGGGACAGGACCCAGATCTATAACAGGGGATC450.33316058340119936No Hit
GGTCTGTGATGGGGGATCTGCACGGGGACAGGACCCAGATCTATAACAGG420.3109498778411194No Hit
CTATAACAGGGGATCTGCCTGGGGATAGAAGCCAGGCCTACAATGGGGGA420.3109498778411194No Hit
GGACAGGACCCAGGTCTGTGATGGGGGATCTGCACGGGGACAGGACCCAG420.3109498778411194No Hit
GTCCACTGTTCAGTTACATTTTGGGGGGAAATCCAATCAGGGCACACCTC410.3035463093210928No Hit
AGCAGGGACAGGACCCAGGTCTGTGATGGGGGATCTGCACGGGGACAGGA400.2961427408010661No Hit
GATAAGGGGCGTCTGTCAGGGCACCTCAGACCTCAAAGACGAGCTGAAGC400.2961427408010661No Hit
AGCAGGGCCCATCCCACGCCTGTCCTGTCCACTGTTCAGTTACATTTTGG400.2961427408010661No Hit
GCACGGGGACAGGACCCAGATCTATAACAGGGGATCTGCCTGGGGATAGA380.2813356037610128No Hit
TCCCATTACTCCCTGTCAAAGCCCTTTGTATCCTTCTTAACACTTTTGTT380.2813356037610128No Hit
ACCCAGATCTATAACAGGGGATCTGCCTGGGGATAGAAGCCAGGCCTACA380.2813356037610128No Hit
ACCCAGGTCTGTGATGGGGGATCTGCACGGGGACAGGACCCAGATCTATA380.2813356037610128No Hit
GGATAGAAGCCAGGCCTACAATGGGGGATCAGGTCTAGACAGGACCCTGC380.2813356037610128No Hit
ATAACAGGGGATCTGCCTGGGGATAGAAGCCAGGCCTACAATGGGGGATC370.2739320352409862No Hit
CTCTAAGGCAGGACGTCACCGAGTGAGGACCGCTCTCTGGCTGGCTGCCC370.2739320352409862No Hit
GGCCCATCCCACGCCTGTCCTGTCCACTGTTCAGTTACATTTTGGGGGGA360.2665284667209595No Hit
ACATAGCACCGTGTTCAACCTTGAGAATTTCTGGTAACAACTCATGGGCT350.2591248982009328No Hit
GGGCACACCTCTAAGGCAGGACGTCACCGAGTGAGGACCGCTCTCTGGCT320.2369141926408529No Hit
GCCTGGGGATAGAAGCCAGGCCTACAATGGGGGATCAGGTCTAGACAGGA310.22951062412082623No Hit
GTGTAGCATTTGACATATAGAAAAGCACTTAGGGAAAATCATTGTGTGAC310.22951062412082623No Hit
AGCGAAAGCAGGGCCCATCCCACGCCTGTCCTGTCCACTGTTCAGTTACA310.22951062412082623No Hit
CTACTAAGCTGTAAGCTTCATGAAAACAGGAACCAGATTTTGTATCCTGA300.2221070556007996No Hit
CCACTGTTCAGTTACATTTTGGGGGGAAATCCAATCAGGGCACACCTCTA300.2221070556007996No Hit
GGGCCCATCCCACGCCTGTCCTGTCCACTGTTCAGTTACATTTTGGGGGG290.21470348708077291No Hit
GCATCTGGGAGGTCGCGGAGAAACATCTTGAGTTGCAGGCATCTTTCTGA290.21470348708077291No Hit
AAGCAGGGCCCATCCCACGCCTGTCCTGTCCACTGTTCAGTTACATTTTG280.20729991856074628No Hit
ACAATGGGGGATCAGGTCTAGACAGGACCCTGCTCTAAACTGGGGGCCCA280.20729991856074628No Hit
GTGTTAAATGATGGGGCTTTCTGGGCAGGCTGTTCTTGGGTGTGATCGAG270.19989635004071965No Hit
GCACTGGGGCTTGAGTTGCATCTGCCCTGCATCTGGGAGGTCGCGGAGAA270.19989635004071965No Hit
GCCCATCCCACGCCTGTCCTGTCCACTGTTCAGTTACATTTTGGGGGGAA260.192492781520693No Hit
CCATTACTCCCTGTCAAAGCCCTTTGTATCCTTCTTAACACTTTTGTTAC260.192492781520693No Hit
AGTGAGGACCGCTCTCTGGCTGGCTGCCCGGGACGCGGGGCCTGGTTCCC250.18508921300066633No Hit
GGATTAGGTAGGAAGAGACTTGGAACATAGAAACTTAAAGAAAATCTTGG250.18508921300066633No Hit
AGCGAAAGCAGGGACAGGACCCAGGTCTGTGATGGGGGATCTGCACGGGG250.18508921300066633No Hit
ATCTAAAATATCTCCCCATCCCATTACTCCCTGTCAAAGCCCTTTGTATC250.18508921300066633No Hit
CATCAGAATAGTGTGAAAGATTCTCATTAGTTTATGAGTGTAGGAGATCA250.18508921300066633No Hit
CAATGAAGGCAGTCAGATCTGGGGCCAGGGACCCCTGGGAACCAGGCCCC240.17768564448063967No Hit
GTCCTAGATTGGATACAATGGGCAAAGAAAGGGGGTGTCAGAGGGGAATG230.170282075960613No Hit
CTCATGGGCTACATGCACAATCTTCTCTTTCTTTCTTTCTTTCTTTTTTT230.170282075960613No Hit
CAACTGGAAGCATGTTGTCCCCCAACAATGAAGGCAGTCAGATCTGGGGC230.170282075960613No Hit
GATCTGGGGCCAGGGACCCCTGGGAACCAGGCCCCGCGTCCCGGGCAGCC220.16287850744058635No Hit
GTTCTTGGGTGTGATCGAGAGGCTGGCTCAGGTGGCTTTCAGATGGACTC210.1554749389205597No Hit
GTCTAGACCTGATCCCCCATTGTAGGCCTGGCTTCTATCCCCAGGCAGAT210.1554749389205597No Hit
CTGATAACCCAGAGTCCATCTGAAAGCCACCTGAGCCAGCCTCTCGATCA210.1554749389205597No Hit
GCGAAAGCAGGGCCCATCCCACGCCTGTCCTGTCCACTGTTCAGTTACAT210.1554749389205597No Hit
GAAAAGCACTTAGGGAAAATCATTGTGTGACTAACTGAATGAATAAATCC210.1554749389205597No Hit
TCTTAACACTTTTGTTACAAGCTGTAATCATTTTACTGATTTCTATGTTC200.14807137040053306No Hit
CCCAAGAACAGCCTGCCCAGAAAGCCCCATCATTTAACACCTAGGCGCCC200.14807137040053306No Hit
ATCATTGTGTGACTAACTGAATGAATAAATCCAACTTTCACCTGTTTTCC200.14807137040053306No Hit
GTCCCCCAACAATGAAGGCAGTCAGATCTGGGGCCAGGGACCCCTGGGAA200.14807137040053306No Hit
CCAAAGACACTGATAACCCAGAGTCCATCTGAAAGCCACCTGAGCCAGCC200.14807137040053306No Hit
CCATAGCACTGGGGCTTGAGTTGCATCTGCCCTGCATCTGGGAGGTCGCG190.1406678018805064No Hit
CAGTTACATTTTGGGGGGAAATCCAATCAGGGCACACCTCTAAGGCAGGA190.1406678018805064No Hit
CATTAGCTGAGTGTGCCAGGGAGCGGGAACAGTGTGGCTTGGGCAATTCT190.1406678018805064No Hit
GACAGGACCCAGGTCTGTGATGGGGGATCTGCACGGGGACAGGACCCAGA190.1406678018805064No Hit
GGACCCAGGTCTGTGATGGGGGATCTGCACGGGGACAGGACCCAGATCTA190.1406678018805064No Hit
GTATATCAGGCAGTCCCAGGACAAGAAGCCCGAGGAACCCACCAGCTCAG190.1406678018805064No Hit
GGACAGGACCCAGATCTATAACAGGGGATCTGCCTGGGGATAGAAGCCAG190.1406678018805064No Hit
GGTGTTGGCTGGCAGTGGGACCAGTGGACTGAGTCAGGAGAAATTTAAGA190.1406678018805064No Hit
CTTCCCTACTAAGCTGTAAGCTTCATGAAAACAGGAACCAGATTTTGTAT180.13326423336047974No Hit
AATCAGGGCACACCTCTAAGGCAGGACGTCACCGAGTGAGGACCGCTCTC180.13326423336047974No Hit
ACAATGAAGGCAGTCAGATCTGGGGCCAGGGACCCCTGGGAACCAGGCCC180.13326423336047974No Hit
TTTCTATGTTCATTGGCTTTTGGTCCATCTTCCCTACTAAGCTGTAAGCT180.13326423336047974No Hit
GGACCCAGATCTATAACAGGGGATCTGCCTGGGGATAGAAGCCAGGCCTA180.13326423336047974No Hit
GTTCAGGAAGGAGGGAATGGATCACATAGCACCGTGTTCAACCTTGAGAA180.13326423336047974No Hit
AAACTGGGGGCCCACAACTGGAAGCATGTTGTCCCCCAACAATGAAGGCA170.1258606648404531No Hit
CCTTGGTGGTGTTTAGTCACTCAATGTTTATTCACTCGAGAGAAAACACT170.1258606648404531No Hit
GTTTAGAGCAGGGTCCTGTCTAGACCTGATCCCCCATTGTAGGCCTGGCT170.1258606648404531No Hit
GTCTCGGTCCATAGCACTGGGGCTTGAGTTGCATCTGCCCTGCATCTGGG170.1258606648404531No Hit
GGATACAATGGGCAAAGAAAGGGGGTGTCAGAGGGGAATGCCAGGTTTCT170.1258606648404531No Hit
ATGTTTATTCACTCGAGAGAAAACACTTGCACATCACAGCTTAGCGTTCT170.1258606648404531No Hit
ATATTAGATCACCCATTCCTACTGCAGACCTATCAAACAATCTTCTGCTA160.11845709632042645No Hit
GCATTTGACATATAGAAAAGCACTTAGGGAAAATCATTGTGTGACTAACT160.11845709632042645No Hit
ATCCCACGCCTGTCCTGTCCACTGTTCAGTTACATTTTGGGGGGAAATCC160.11845709632042645No Hit
CCCCAAATGTTCCCTACTTTAGTGAAGCTTTCTCTAACAAGCCTATCTAA160.11845709632042645No Hit
GGATCTGCCTGGGGATAGAAGCCAGGCCTACAATGGGGGATCAGGTCTAG160.11845709632042645No Hit
GTGTTTAGTCACTCAATGTTTATTCACTCGAGAGAAAACACTTGCACATC160.11845709632042645No Hit
GTCAAATGCTACACGATGCTGGGTGTTCAGGATACAAAATCTGGTTCCTG160.11845709632042645No Hit
GTGTTCAACCTTGAGAATTTCTGGTAACAACTCATGGGCTACATGCACAA160.11845709632042645No Hit
ATATCAGGCAGTCCCAGGACAAGAAGCCCGAGGAACCCACCAGCTCAGGT160.11845709632042645No Hit
GATCTAAGACGTTGTCAGCCCACTGTGAATCCTGCAGTGGTTTCCCGTGA160.11845709632042645No Hit
GTATCCTTCTTAACACTTTTGTTACAAGCTGTAATCATTTTACTGATTTC160.11845709632042645No Hit
GCCTACAATGGGGGATCAGGTCTAGACAGGACCCTGCTCTAAACTGGGGG160.11845709632042645No Hit
ACTGGAAGCATGTTGTCCCCCAACAATGAAGGCAGTCAGATCTGGGGCCA160.11845709632042645No Hit
GGCAGGACGTCACCGAGTGAGGACCGCTCTCTGGCTGGCTGCCCGGGACG160.11845709632042645No Hit
CTTCTTAACACTTTTGTTACAAGCTGTAATCATTTTACTGATTTCTATGT150.1110535278003998No Hit
CTCTTGTGCTCAGTCACTAGCTGGAACAGCCAGACCTTGGCATGGACGTC150.1110535278003998No Hit
CTTCAGGGACCTGCCATTTAGGAAAACAGGTGAAAGTTGGATTTATTCAT150.1110535278003998No Hit
CATCTTGAGTTGCAGGCATCTTTCTGAAAACATTTCTGGGCGCCTAGGTG150.1110535278003998No Hit
AAGCAGGGGTTGGCATTACGGCCTGCAGGGGCACGCAGGGCAGGGCAGGA150.1110535278003998No Hit
GTAAAATCTGGTCCTAGATTGGATACAATGGGCAAAGAAAGGGGGTGTCA150.1110535278003998No Hit
CTCAATGTTTATTCACTCGAGAGAAAACACTTGCACATCACAGCTTAGCG150.1110535278003998No Hit
CCCCAAAACTAGCCATTCCCCACCAAAGACACTGATAACCCAGAGTCCAT150.1110535278003998No Hit
CCAGTGGACTGAGTCAGGAGAAATTTAAGAAGTAAAATCTGGTCCTAGAT150.1110535278003998No Hit
TCTATAACAGGGGATCTGCCTGGGGATAGAAGCCAGGCCTACAATGGGGG150.1110535278003998No Hit
ATTGGATACAATGGGCAAAGAAAGGGGGTGTCAGAGGGGAATGCCAGGTT150.1110535278003998No Hit
ATGCTGGGTGTTCAGGATACAAAATCTGGTTCCTGTTTTCATGAAGCTTA150.1110535278003998No Hit
GAAAGGGGGTGTCAGAGGGGAATGCCAGGTTTCTCTGACCCCAAAACTAG140.10364995928037314No Hit
GAGTTGCATCTGCCCTGCATCTGGGAGGTCGCGGAGAAACATCTTGAGTT140.10364995928037314No Hit
AATCCAATCAGGGCACACCTCTAAGGCAGGACGTCACCGAGTGAGGACCG140.10364995928037314No Hit
CTATAAAATATCCTACATTCTAACATTCTTCTTACTTAACTGCATTCTGC140.10364995928037314No Hit
AAGAAAGGGGGTGTCAGAGGGGAATGCCAGGTTTCTCTGACCCCAAAACT140.10364995928037314No Hit
CCACCAAGGTGGTGATCAATCTGTCAGCCTGTTGTCTTCAGGGACCTGCC140.10364995928037314No Hit
GGACGTCACCGAGTGAGGACCGCTCTCTGGCTGGCTGCCCGGGACGCGGG140.10364995928037314No Hit
CTTTTGTTACAAGCTGTAATCATTTTACTGATTTCTATGTTCATTGGCTT140.10364995928037314No Hit
AAGCTGTGATGTGCAAGTGTTTTCTCTCGAGTGAATAAACATTGAGTGAC140.10364995928037314No Hit
ATTCTCTTGTGCTCAGTCACTAGCTGGAACAGCCAGACCTTGGCATGGAC140.10364995928037314No Hit
GGGCAAAGAAAGGGGGTGTCAGAGGGGAATGCCAGGTTTCTCTGACCCCA140.10364995928037314No Hit
GCTGTAAGCTTCATGAAAACAGGAACCAGATTTTGTATCCTGAACACCCA140.10364995928037314No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAAGGG100.0070141144145.02
GATAAGG100.0070141144145.01
GGGGCGT100.0070141144145.06
GGGCGTC100.0070141144145.07
TAAGGGG100.0070141144145.03
GCAGGGC259.1860077E-487.03
AAGAAGG259.1860077E-487.0145
CTACACG450.00943600148.3333329
TGTCCAC604.7726318E-448.3333323
GCTACAC450.00943600148.3333328
ATGCTAC450.00943600148.3333326
TGCTACA450.00943600148.3333327
AATGCTA450.00943600148.3333325
CCAGGTC450.00943600148.3333325
AAATGCT450.00943600148.3333324
CTGTCCA657.0814625E-444.6153832
TCCACTG700.001020002541.428575
GTCCACT700.001020002541.428574
CTGTTCA750.00143209838.6666649
ACTGTTC800.001966400336.258
Produced by FastQC (version 0.11.5)