FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n01_S1_R1_F05-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n01_S1_R1_F05-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences923450
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTAATACCTATCAAAATACCAATAACACTTCTCACAGAACTAGAACAA11320.12258378905192484No Hit
GTTTATATGAGAAAGGAGGGGAATGAAAGAGAAAATAGAATCAAAACACC10130.10969733066219069No Hit
GTATAGACATCTTAACAATATTGTTCTTCCAATCCATGAGCATGGATTGT10070.10904759326438897No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA2150.050.5968321
ATAGACC6000.029.0057073
CGAAAGC4050.028.6476122
GCGAAAG4200.027.6274761
TATATAC8250.027.2477863
TATAGAC12750.026.162012
CCTCGCG3151.033186E-925.318329145
GTATATA8950.025.1193941
GTATAGG2651.3720273E-724.6301571
GTATTAC11000.023.7345141
ATATACT6850.023.2892554
TAGTACC2852.7730493E-722.8992444
GAGCGAA2552.6995476E-622.7520391
TACCTAT8950.022.6811147
TAGACCT8450.022.3120844
ACCTATC9050.021.6294068
TATTACA12450.021.5504262
TATACTT6450.021.3607925
TATATAT9200.021.281362
AGTACGT1750.003658940820.7138758
Produced by FastQC (version 0.11.5)