FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n01_S1_R1_F03-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n01_S1_R1_F03-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1043078
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG18850.1807151526539722No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA16180.155117833949139No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT13760.13191726793202427No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC13330.1277948533091485No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA12360.11849545288080086No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT11740.11255150621525907No Hit
GGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATG10680.10238927481933278No Hit
CTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTGAA10450.1001842623466318No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGGCG400.006049691754.37788145
AGGTCGT3850.048.970676
ATATTCA9400.047.828564
GGTCGTT3800.047.695327
TATATTC9550.047.0773283
CAATTCG5550.047.0250059
GTAGAAA9800.044.3964841
AGTAGAA8550.038.16541
GTATTAA1553.464629E-837.4267161
TCGTTTT5050.035.889559
TTATAGG4200.034.53061
TTTAAAC10750.033.7275663
CGAAAGC1956.9794623E-933.468123
TATAGAC8150.032.9205863
AGCGAAA2008.934876E-932.631421
ATTATAT4150.031.4519731
GGTTGCG950.00472616430.527931145
TATGTTG5550.030.0509595
AATATAT11550.028.2523121
TCAATAT15400.028.245548
Produced by FastQC (version 0.11.5)