FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n01_S1_R1_E11_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n01_S1_R1_E11_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences308647
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGGATAGCAAATAGTAGCATTGCCACAGCTATCTCAATGCATGTGTT9220.2987231367873331No Hit
GCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCATTCTGCTGT8400.27215556930733165No Hit
ATATACAAGAGAGTAGATGGAAAGTGGATGAGGGAACTTGTCCTTTATGA7830.253687869961477No Hit
CTTAATATGAGTGCAGACCGTGCCAGAAAGGTGAGATCTTCGATCTCAGC7100.23003625500976843No Hit
GAATACTGACTTAGCTAATAACGTCCTGCATACTTTCCCAATGGAACCTT6250.20249670335366943No Hit
GATCTATGCTGGGAGTCAGCAATCTGTTCACAGGTTGCGCATATTAGGCC6210.2012007244522059No Hit
CATTATGATCTGGCATTCCAATTTGAATGATACAACTTACCAGAGGACAA5950.19277686159269328No Hit
ATTGAATACTGACTTAGCTAATAACGTCCTGCATACTTTCCCAATGGAAC5850.18953691433903458No Hit
AGCAGGTACTGATTCGAAATGGAAGATTTTGTGCGACAATGCTTCAATCC5610.18176104093025366No Hit
CTCATAAGCAATCCTTGTTTTTCTTCCATTCTCACCCCTCCAGAAGTTTC5310.17204119916927751No Hit
CTATTGAATACTGACTTAGCTAATAACGTCCTGCATACTTTCCCAATGGA5220.1691252466409847No Hit
CACTTGGAAGTATGCTTCATGTATTCAGATTTTCATTTCATCAATGAGCA5030.16296934685903314No Hit
CCCATATACAAGAGAGTAGATGGAAAGTGGATGAGGGAACTTGTCCTTTA5020.16264535213366726No Hit
CTATGGTATCAAGTACTCTTGAACTGAGAAGCAGGTACTGGGCCATAAGG5010.1623213574083014No Hit
CCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCATGTCAAAG4960.16070138378147203No Hit
GGCAATGAAAGAGTATGGAGAGGATCTGAAAATCGAAACAAACAAATTTG4920.15940540488000857No Hit
TAATAGTAGAGCCTGAAGACCCAAACGCACTTTTGAAGCACAGATTTGAA4760.1542214892741546No Hit
CATTAATACTGCTTTGCTTAATGCATCCTGTGCAGCAATGGATGATTTCC4730.15324950509805702No Hit
GCATAAATCTCATAGGCAAATGGTGACAACAACCAATCCATTAATAAGAC4710.15260151564732527No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAGCTATCTCAA4480.14514963696391023No Hit
GGCATGGAGAGGATCTGAAAATCGAAACAAACAAATTTGCAGCAATATGC4300.13931773190732455No Hit
TGTCAAAGGAGGGCACGATCGGGTTCGCTGCCCTTTCGTCCGAGAGCTCG4200.13607778465366582No Hit
GTCCCTAAGAGCCTGAACAATAAGGAGCAATTTTCTTGATTCAGCTGAAA4130.13380982157610474No Hit
CCTTTGGGTGACTCTCCAATGGGCCATGTTTCTGATCTATTCTCAAAAAA3860.1250619639912262No Hit
GCATAGATCTCATAGGCAAATGGTGACAACAACCAATCCATTAATAAGAC3860.1250619639912262No Hit
GATTTATGCTGGGAGTCAGCAATCTGTTCACAGGTTGCGCATATTAGGCC3830.1240899798151286No Hit
CCCTAGCTCCAGCGCTGGTCTGAAAAATGATCTTCTTGAAAATTTGCAGG3760.12182201673756751No Hit
GTTCCAGATTGTCCCTAAGAGCCTGAACAATAAGGAGCAATTTTCTTGAT3750.12149802201220164No Hit
ATTCAATAGTCTGTATGCATCTCCACAATTAGAAGGATTTTCAGCTGAAT3590.1163141064063477No Hit
GTTGTATATGAGGCCCATACAACTGGCAAGTGCACCAGCAGAATAACTGA3550.11501812750488422No Hit
CTCTAGCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCATTCT3510.11372214860342074No Hit
AATATGCACTCACTTGGAAGTATGCTTCATGTATTCAGATTTTCATTTCA3400.11015820662439615No Hit
ATATACAACAGGATGGGGGCTGTGACCACCGAATCGGCATTTGGCCTAAT3330.10789024354683505No Hit
AATATGGACAGAGCCGTTAAACTGTATAGAAAGCTTAAGAGGGAGATAAC3280.1062702699200057No Hit
CGTTTAAGGAGACGATAAATGCATTTGAAAAAAAGGCGATCAAGAATCCA3230.10465029629317636No Hit
GTAGTATTCAATAGTCTGTATGCATCTCCACAATTAGAAGGATTTTCAGC3140.10173434376488351No Hit
GAACAATAAGGAGCAATTTTCTTGATTCAGCTGAAAATCCTTCTAATTGT3130.10141034903951765No Hit
GTTGTAAACAGTATTTGCAACACCACAGGAGCTGAGAAACCAAAGTTTCT3110.1007623595887859No Hit
CTCATAGACTCTGGTACTCCTTCCGTAGAAGGCCCTCTTTTCAGACCGCG3100.10043836486342002No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTATGC100.0070836716145.033073
GTGTGAC100.007087105145.00957145
GGAGTTA100.007087105145.00957145
AGCGAAA351.895387E-9124.3140641
CATAGAA203.8599924E-4108.77482
CCATAGA203.8599924E-4108.77481
GAGTTAT203.8599924E-4108.77481
AGTTATG301.5958904E-596.6887052
ATAGAAT259.372058E-487.0198443
CGTCCTA259.372058E-487.0198441
CCCAACA801.8189894E-1281.58112
GTAGTAT554.377398E-879.108941
CAAGGTA1550.074.831529
TCCCAAC903.6379788E-1272.516531
GGTCGTT300.001935207872.493037
AACACTG955.456968E-1268.699875
GTAGAAA2150.064.084382
GTCGTTT350.003565524562.1368878
TAATCAC606.7853034E-660.4304433
GAGCTAA606.79836E-660.410869
Produced by FastQC (version 0.11.5)