FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n01_S1_R1_E09_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n01_S1_R1_E09_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences86
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCAAAAGAAGTGAACGCCAAAATTGAACCATTCTTGAGGACGACACCAC11.1627906976744187No Hit
GTGACTCTCCGATTGGCCATGGTTCCGGTTACATATACTGTTCACCACTG11.1627906976744187No Hit
CCAATACTCATGTCAGCTGATTCATTTACTCCAGACACTCCAAAGCTGGG11.1627906976744187No Hit
AGTCTCGATACTGAATCTTGGACAAAAGAAATACACCAAGACAATATACT11.1627906976744187No Hit
CTGCACACTTTCCCAATAGAGCCTACCTCCACTCCCCTGGGGGACTCTCC11.1627906976744187No Hit
CTGCTGCTCTTCTTACTATGTTTCTAGCAGCGATAATCAAACTTTGGTCA11.1627906976744187No Hit
GCACCCCAAGAGCTCTGATTCTTAAATGTTTTGTTTACTGAAATACACAG11.1627906976744187No Hit
GTCCTCTACCCCAGGTCGAAACGTACTTTCTCTCTATCATACCGTCAGGC11.1627906976744187No Hit
ATGTTGACCAAAGTTTGATTATCGCTGCTAGAAACATAGTAAGAAGAGCA11.1627906976744187No Hit
GGATGCAGGTGCATTGACTGAAGATCAAGATCAAGGAACATCTGGGCTGG11.1627906976744187No Hit
GAATAGAACGATTCCTCTTGGGAATCCAGGAATGTGTAGTTGCAACGGCA11.1627906976744187No Hit
GTGCCAAGGAGGCACAGGATGTGATTATGGAAGTTGTGTTCGCATTTGAG11.1627906976744187No Hit
ATCCATAGCGATAAAAAAAGCTTGTGAATTCAAATGTCCCTGTCTTATTT11.1627906976744187No Hit
CTCTTGTCCCTAAGGCAACCAGAAGCCGGTACAGTGGATTCGTAAGGACA11.1627906976744187No Hit
GGACGCCACCATCAAGGGAGCAATTTTACAATCCTGGAGCTCTTCTTTCT11.1627906976744187No Hit
GGATACACCATGGACACAGTAACCAGCACACACCAATACTCAGAAAAGGG11.1627906976744187No Hit
GACCCATTAGCATCTCTCTTGGAAATCTGCCACAGCACACAGATTGGAGG11.1627906976744187No Hit
GTCCTGTAGAATCTGTCAACTCCTGCTTGTATTCCCTCATGGTTTTGTGC11.1627906976744187No Hit
ATGCTAAAATTAGCCACAAATCCATAGCGATAAAAAAAGCTTGTGAATTC11.1627906976744187No Hit
ATATAGTGGTATTCACTCCCCCTCGTGACTCTGGTCTTCAATACTTAATT11.1627906976744187No Hit
GAGCAAGGCCGTATTTATGTACACTCACTTCATTATGTATTCAGTAGCCC11.1627906976744187No Hit
CGGCTTCCGATGTGGATCGCCTCGATAATAACAAGTTGGTCAACATACTC11.1627906976744187No Hit
GTACGTTTTTGGACAGTATGGATAGCAAATAGTATCATTGCCACAACTCC11.1627906976744187No Hit
CTGGGAGCCCAGACTGTTCAAGCTTTTCGCAAATGCTCCTAGCTAAAGTT11.1627906976744187No Hit
AGAAGGCAGCGCCTCATTTCCATGCCCCATTTCATCTTGATCTTGGAGGT11.1627906976744187No Hit
CAATTGAAGAGCCATCTGGGCCGTTGCAGGTCCAACGTCATTGTTTATCA11.1627906976744187No Hit
CGCGAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAAATGAG11.1627906976744187No Hit
GTTAGGCTCTTTCCAGCCTAAGAATGTCTTCCTGCATTTGATTGCATCAT11.1627906976744187No Hit
GTAGCAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCC11.1627906976744187No Hit
GTGTTTATATTGAAGTGTTGCACTTAACCCAAGGGACGTGCTGGGAGCAG11.1627906976744187No Hit
CCAAGTGGGTATGCACAAACAGACTGTGGTCTAGAGGCTATGGCTTTCCT11.1627906976744187No Hit
TAATATCGCAGTCCCGCAATCGAGAGATACTAACTAATAACACTGTGGAC11.1627906976744187No Hit
GAATGAAGTGGATGATGGCAATGAGATACCCAATTACAGCAGACAAGAGA11.1627906976744187No Hit
GTTTAATGTCCAATCATAAGTCTGGCGACCTTGAGTTAGTTTATCTACCC11.1627906976744187No Hit
GAACAATGAGAAGCAATTTTCTAGATTCAGCCGAAAACCCCTCAAGTTGT11.1627906976744187No Hit
TCCCTGTCTTATTTATATAGGACTTCTTTTTGCTCATGTTGATTCCCACT11.1627906976744187No Hit
GTCTATATGCGTCTCCACAACTTGAGGGGTTTTCGGCTGAATCTAGAAAA11.1627906976744187No Hit
GAAAGGCATAAATCCCAATTACCTCATGGCTTGGAAGCAGGTGCTAGCAG11.1627906976744187No Hit
GGGGCCTATATGCACTGCTAGGGAACAGTTTATCGCATGGAGTGACGCAC11.1627906976744187No Hit
ATAGAAGTCTTTAGATCGAATGGCCTAACAGCTAATGAGTCAGGAAGGCT11.1627906976744187No Hit
CCCCAGTTCTGGAAAAGCACTTTTGCATCTTTTTGGAAATGCCTCAAGAG11.1627906976744187No Hit
CCCATAATGTTCTCAAAAAAACAGGCAATACAAGGGAAACGTTACATGTT11.1627906976744187No Hit
GTTTATCATGTTGTTCTTTATCACTGTTACTCCAATACTCATGTCAGCTG11.1627906976744187No Hit
CTTTATGACTGACAAAGGGATTCAGGGGATTACAAAGTCTTCCCCGATAA11.1627906976744187No Hit
ATCATGCAATACTCCTGTGAGAATACCATGGCCACAATTATTGCCTCAGC11.1627906976744187No Hit
GCAATAGGGTTGAGGATTAGCTCATCTTTCAGTTTTGGTGGGTTCACTTT11.1627906976744187No Hit
TCAATGATGTGGGAGATCAATGGCCCTGAGTCAGTGCTAGTCAACACTTA11.1627906976744187No Hit
AATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCAC11.1627906976744187No Hit
CCCTTATACTGGAGATCGTCCATACAGCCATGGAACAGGAACAGGATACA11.1627906976744187No Hit
ACATTAAATACGATGACCAAAGATGCAGAGAGAGGCAAGTTAAAAAGAAG11.1627906976744187No Hit
TCCCAGGACTCAGTGATGCTGTGCCATCTATTAGAAGAGGCCTTATTTTC11.1627906976744187No Hit
GAAGTGTCCCACTGCAGGGCTACTGAATACATAATGAAGGGAGTGTACAT11.1627906976744187No Hit
CAACTACTTCAGTCCATGTGTGAGGATGGAGTTGAACCAATATGCATTAA11.1627906976744187No Hit
TCGGATGTCCGTCCTTCATTGTTCCCGCTGAATGCTGCCATAACGGTTGC11.1627906976744187No Hit
GGCAAGAGAAGAACCCCGAACTAAGATTTAATTGGATGATGGCAATGAGA11.1627906976744187No Hit
GTTGATTTCTGTTTAATGTCCAATCATAAGTCTGGCGACCTTGAGTTAGT11.1627906976744187No Hit
GGTCTATCCCGACCAGTGAGTACCCTTCCCTTTCAAAGTCATGCCCACTT11.1627906976744187No Hit
ATACTGGAGATCCTCCATACAGCCATCGAACAGGAACAGGATACCCCATA11.1627906976744187No Hit
GAATACTGGAGAATTGCCTCTTACCAGTATCCTCAACCCTGATCCTCTCA11.1627906976744187No Hit
TCATCATCCATTAGCTCCCATTTTAAGCAGACCTCAGGAATGTGAAGATT11.1627906976744187No Hit
ATTACATCATTTAATAAATCTATTAGCCTTCCTGACTCATTAGCTGTTAG11.1627906976744187No Hit
CCCTCCCCCTCGTGACTCGGGACTTCAATACTTAATTTCAGAGCATCCAT11.1627906976744187No Hit
ATGCCATAAGCACCACATTCCCTTATACTGGAGATCCTCCATACAGCCAT11.1627906976744187No Hit
TTGTTGTATAACATTGTGGGATCTTGGGACCATTGAATTTTCACATTTTC11.1627906976744187No Hit
GTATCACCTCTGGCCGTAACATGGTGGAATAGGAATGGCCCAACAACAAG11.1627906976744187No Hit
AGTATGGATAGCAAATAGTAGCATTGCCAAAACTACTTCAGTGCATGTGT11.1627906976744187No Hit
CTGTTGAATACAGATTTTGCCAGTAAGGTCCTGCACACTTTCCCAATAGA11.1627906976744187No Hit
CGCAAAGAACAATAGGGAAGAAAAAACAAAGACTGAATAAGAGAGGCTAT11.1627906976744187No Hit
GAATTCCCCTTTGGCTTGTGTTGAGAATAGAACGATTCCTCTAGGGAATC11.1627906976744187No Hit
CAGCACTTCTGGTACATCTGTTCATCCTCAAGAATTCCCCTTTGGCTTGT11.1627906976744187No Hit
TTCTTGATCCGTCCAGACTCGAAGTCGACACTTGCATCAATCCGGGACCT11.1627906976744187No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTG11.1627906976744187No Hit
GAATGGATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGAGCAAAAT11.1627906976744187No Hit
CAATATAAATAAGACAGGGACATTTCAATTCACAATCTTTTTTTATCGCT11.1627906976744187No Hit
CACCTATAATGTTCTCAAACAAAATGGCAAGACTAGGGAAAGGGTACATG11.1627906976744187No Hit
ATCCATGGCTGCACAGGATGCATTGAGCAAGGCCGTATTTATGTACACTC11.1627906976744187No Hit
CCTATATGTGTATCTGTAGTCTTTGATGAACAATTGAAGAGCCATCTGGG11.1627906976744187No Hit
GAGAGATCTAATGTCGCAGTCCCGCCCTCGCGAGATACTCAGTAAGACCA11.1627906976744187No Hit
GTTTCCCTAGTGTTGCCTGTTTGTTTGAGCAGATTATGGGTGCCATGCTC11.1627906976744187No Hit
ATACTGATGTGGTGAACTTTGTAAGTATGGAGTTCTCACTTACTGACCCG11.1627906976744187No Hit
AGGCTAATGTGCTAATTGGGCAAGGGGACGTAGTGTTGGTAATGAAACGA11.1627906976744187No Hit
CATCAGGAAGGCAAGAGAATAACCCCCCACTCACAATGAAGTGGATGATG11.1627906976744187No Hit
GCTACAGGACATTGAAAATGAAGAGAAGATCCCAAGGACAAAGAACATGA11.1627906976744187No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC11.1627906976744187No Hit
ACCTGCCGCACCAGCAGTTATCGGAACAGTTGCACCTTCAATTTCAGCGC11.1627906976744187No Hit
GTCCCGCACTCGCGAGATACTCACTAAGACCACTGTGGACCATATGGCCA11.1627906976744187No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCAATA50.0145.05
GCAATAC50.0145.06
CAATACT50.0145.07
TCATGCA50.0145.02
ATCATGC50.0145.01
ATGCAAT50.0145.04
AATACTC50.0145.08
ATACTCC50.0145.09
CATGCAA50.0145.03
AATTATT100.072.5145
Produced by FastQC (version 0.11.5)