FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n01_S1_R1_E07-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n01_S1_R1_E07-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences828163
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATATATACTTACACAGTTTGAGCATTCACAGGCCACTAGAAGCCCATCT8650.10444803740326482No Hit
TGTATATACAGCTCGCTGAAGAGGTCCATGACCTCAAAAAAGATCAAATG8580.1036027931699436No Hit
GTATATACATACATATATACTTACACAGTTTGAGCATTCACAGGCCACTA8350.10082556211760245No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA2300.040.981421
CGAAAGC2800.036.2506033
CCTAACG950.00472644530.5268272
AGTCGCT1204.057053E-430.2088387
TATACAG6100.027.3365215
AGCGATA1358.0807455E-426.8522998
ACTATAC3005.857146E-1026.5837763
TATATAC13650.026.0260753
GTATTAA4400.024.7178131
CGGGCCG1801.5185612E-424.169987145
GCGAAAG3902.0008883E-1124.1670722
TAGGCTA1500.001493911624.167075
TATATAT8850.023.757462
CCTATAC1550.001808052423.3874873
GTATATA11200.023.3053671
ACGACTG2802.3412395E-723.303965
GTATATG3452.9613147E-923.1177231
GTATAGG1902.191582E-422.8965031
TATTACA9050.022.4313142
ATATACT7800.021.3785634
Produced by FastQC (version 0.11.5)