FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n01_S1_R1_E04-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n01_S1_R1_E04-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences328288
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGGATAGCAAATAGTAGCATTGCCACAGCTATCTCAATGCATGTGTT9920.30217370114046205No Hit
GCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCATTCTGCTGT9350.2848108977483185No Hit
ATATACAAGAGAGTAGATGGAAAGTGGATGAGGGAACTTGTCCTTTATGA8170.24886684862072328No Hit
CTTAATATGAGTGCAGACCGTGCCAGAAAGGTGAGATCTTCGATCTCAGC7370.22449800175455698No Hit
GATCTATGCTGGGAGTCAGCAATCTGTTCACAGGTTGCGCATATTAGGCC6580.20043376547421776No Hit
GAATACTGACTTAGCTAATAACGTCCTGCATACTTTCCCAATGGAACCTT6470.19708304903011986No Hit
CATTATGATCTGGCATTCCAATTTGAATGATACAACTTACCAGAGGACAA6160.18764012086948045No Hit
ATTGAATACTGACTTAGCTAATAACGTCCTGCATACTTTCCCAATGGAAC6080.18520323618286383No Hit
AGCAGGTACTGATTCGAAATGGAAGATTTTGTGCGACAATGCTTCAATCC5800.1766741397797056No Hit
CTCATAAGCAATCCTTGTTTTTCTTCCATTCTCACCCCTCCAGAAGTTTC5490.1672312116190662No Hit
CTATTGAATACTGACTTAGCTAATAACGTCCTGCATACTTTCCCAATGGA5490.1672312116190662No Hit
CACTTGGAAGTATGCTTCATGTATTCAGATTTTCATTTCATCAATGAGCA5390.1641851057607954No Hit
CCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCATGTCAAAG5280.16083438931669755No Hit
CTATGGTATCAAGTACTCTTGAACTGAGAAGCAGGTACTGGGCCATAAGG5260.16022516814504337No Hit
GGCAATGAAAGAGTATGGAGAGGATCTGAAAATCGAAACAAACAAATTTG5230.15931133638756215No Hit
CCCATATACAAGAGAGTAGATGGAAAGTGGATGAGGGAACTTGTCCTTTA5170.15748367287259968No Hit
CATTAATACTGCTTTGCTTAATGCATCCTGTGCAGCAATGGATGATTTCC5020.1529145140851935No Hit
GCATAAATCTCATAGGCAAATGGTGACAACAACCAATCCATTAATAAGAC5020.1529145140851935No Hit
TAATAGTAGAGCCTGAAGACCCAAACGCACTTTTGAAGCACAGATTTGAA4970.1513914611560581No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAGCTATCTCAA4810.14651769178282484No Hit
GGCATGGAGAGGATCTGAAAATCGAAACAAACAAATTTGCAGCAATATGC4490.1367701530363583No Hit
TGTCAAAGGAGGGCACGATCGGGTTCGCTGCCCTTTCGTCCGAGAGCTCG4380.13341943659226047No Hit
GTCCCTAAGAGCCTGAACAATAAGGAGCAATTTTCTTGATTCAGCTGAAA4330.13189638366312506No Hit
GCATAGATCTCATAGGCAAATGGTGACAACAACCAATCCATTAATAAGAC4130.1258041719465835No Hit
GATTTATGCTGGGAGTCAGCAATCTGTTCACAGGTTGCGCATATTAGGCC4030.12275806608831269No Hit
CCTTTGGGTGACTCTCCAATGGGCCATGTTTCTGATCTATTCTCAAAAAA3980.12123501315917731No Hit
CCCTAGCTCCAGCGCTGGTCTGAAAAATGATCTTCTTGAAAATTTGCAGG3920.11940734964421484No Hit
GTTCCAGATTGTCCCTAAGAGCCTGAACAATAAGGAGCAATTTTCTTGAT3900.11879812847256069No Hit
CTCTAGCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCATTCT3820.11636124378594404No Hit
GTTGTATATGAGGCCCATACAACTGGCAAGTGCACCAGCAGAATAACTGA3770.11483819085680866No Hit
ATTCAATAGTCTGTATGCATCTCCACAATTAGAAGGATTTTCAGCTGAAT3700.11270591675601911No Hit
ATATACAACAGGATGGGGGCTGTGACCACCGAATCGGCATTTGGCCTAAT3570.10874597914026708No Hit
AATATGCACTCACTTGGAAGTATGCTTCATGTATTCAGATTTTCATTTCA3530.10752753679695876No Hit
CGTTTAAGGAGACGATAAATGCATTTGAAAAAAAGGCGATCAAGAATCCA3410.10387220976703382No Hit
AATATGGACAGAGCCGTTAAACTGTATAGAAAGCTTAAGAGGGAGATAAC3400.10356759918120674No Hit
GTAGTATTCAATAGTCTGTATGCATCTCCACAATTAGAAGGATTTTCAGC3360.10234915683789844No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA256.453667E-6116.016941
ATATTCA352.755187E-7103.5865554
GTGGTCC259.381327E-486.99944145
GTACTGA1200.078.553146
GTAGTAT651.5588739E-978.0883261
GTAGAAA1700.072.510592
GGTCGTT406.656735E-572.499547
TTATATT554.052019E-665.918722
TAGATAT753.7495738E-758.0084723
GTCGTTT502.0083092E-457.999638
AGGTAGT902.0414518E-856.3971256
CAAGGGT902.0439984E-856.3885358
TACTGAT1700.055.440837
GGGTAGA400.00604378754.382941
AAGGGTG400.006047431454.3746579
CCTGGTT400.006047431454.374657145
GGTAGTT953.1308446E-853.4207157
GCTGACA2950.049.1522338
TAATGTA1056.8796E-848.3403935
CAAGGTA1650.048.3330279
Produced by FastQC (version 0.11.5)