FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n01_S1_R1_E01_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n01_S1_R1_E01_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTCAATTATATTCAATAAGGAAAGAATAAAAGAAATAAGAAATCAAATG12.857142857142857No Hit
GCCTTCATCTGGGTCTTCAGTTAAAGTGCCAGCATCCTTTCCGAGAACAG12.857142857142857No Hit
TTATTGGAGATGTGCCACAGCACACAGATTGGTGGAATTAGGATGGTAGA12.857142857142857No Hit
GATATATACCTAACAAAGAGTATCCCTCATTATCGAACTCGAAACAAATA12.857142857142857No Hit
CATTTGACTCAAGGAACATGCTGGGAACAGATGTATACTCCAGGAGCGCA12.857142857142857No Hit
GGTAAATACCTATAAATGGATCATCAGAAACTGGGAAACTTTTAAAATTC12.857142857142857No Hit
TAGGTACTAAAGACTGAAATGGTACAAATTCCATTTTAATGTATAGCATT12.857142857142857No Hit
TAACGGAAATGATTCCTGAGAGAAATGAGCAAGGACAAACTTTATGGAGT12.857142857142857No Hit
GCGTTTATAATACAATCATCTTGTGAAAATACACTGGCCACAATTATTGA12.857142857142857No Hit
CTCCCATTTTGATCACTCTTATACCAATCATATCCAACTCCCTCCTTGCG12.857142857142857No Hit
GATGGTATCACCTCTGGCTGTGACATGGTGGAATAGGAATGGACCAATAA12.857142857142857No Hit
ATAACAAATACAGTTCATTATCCAAAAATCTACAAAACTTATTTTGAAAG12.857142857142857No Hit
GATATATGTAATCCTCGCCAGGAACATTCCACGAAATTGATTGTCATTCC12.857142857142857No Hit
CATCTATGGACAAGGAAAATGAACTAGCATGCTCAATCTTTCACTATAAT12.857142857142857No Hit
GGATTGTATGATAAAAGCAGTCAGACGTGATCTGAATTTCGTCAATAGGG12.857142857142857No Hit
GTGCCACAGCGCACAGATTGGTGGAATTAGGATGGTAGACATCCTTAGGC12.857142857142857No Hit
CTGTCGCATCCTTCTGAAAATGTCTTAAAAGTTTATGAATAGGATTCAAT12.857142857142857No Hit
GTATAGCATTGTAGGGTTCTGGGACCAGTGAATTTTAACGGTGTCCCACT12.857142857142857No Hit
CTGGCTGTCAGTAAGTATGATAGAGTCCCGGTTACCTTTAATTACCAAAA12.857142857142857No Hit
ATTGATAGGTATTGACCAACACTGATTCAGGACCATTAATCTACCACATC12.857142857142857No Hit
AAGTCCAACACTTAAATATAAACACTGCATGTTCCGCCGGCTACTGGGAG12.857142857142857No Hit
GAAGAAAAGAGTGCATGACGGATATGAAGAGTTAACAATCATACGGAAAA12.857142857142857No Hit
CAAGCAGTGTGTACATTGAAGTGTTGCATTTGACTCAAGGAACATGCTGG12.857142857142857No Hit
AACAAGCAGTGTGTACATTGAAGTGTTGCATTTGACTCAAGGAACATGCT12.857142857142857No Hit
CAATGGTTGGGAGAAGAGAAACAGACATACTCAGAAAATAAACCAGGAGA12.857142857142857No Hit
AATGTACACACTGCTTGTTCCACCAGCCACTGGGAGGAATCTCGTTTTGC12.857142857142857No Hit
GAATCAAAACACCGTGGTGAACTCCTTGGAGAAGGACCTGCATGGTGGGG12.857142857142857No Hit
GTCTCTTAGTGGTCTTGTTGTAATTGAATACTGGAGAATTACCTCTTACA12.857142857142857No Hit
TGATTACATCCTGTGCGTCCTTGGGAGTGAGATCTGCCTGCCGAGGATGT12.857142857142857No Hit
CGTCGTGGCCTGGTTATAGTTGAATACAGGAGAATTTCACCTTAAAAGTA12.857142857142857No Hit
CTTTAGTACCTAAGGCCATTAGAGGGCAATACAGTGGGTTTGTAAGATCT12.857142857142857No Hit
ACTCAGAAAAGCAACCAGGAGATTGATTCAGCTGATAGTGAGTGGGAGAG12.857142857142857No Hit
GGATACACCAAGGACACAGTAAACAGAACAGACCATTTCTCAGAAATTGG12.857142857142857No Hit
CTTCATATACCTCATGCACTCTTATATTCAATGTTTCAACATTGCCCGTA12.857142857142857No Hit
GTAGATACAAGGGGAAAGGCTGGCCTGTAGGGAACAAGGGAGGGACAAGA12.857142857142857No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)