FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n01_S1_R1_E01-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n01_S1_R1_E01-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences329689
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATACAAGAGAGTAGATGGAAAGTGGATGAGGGAACTTGTCCTTTATGA7460.22627385202418035No Hit
CCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCATGTCAAAG7340.22263405815783968No Hit
CTTAATATGAGTGCAGACCGTGCCAGAAAGGTGAGATCTTCGATCTCAGC7180.21778099966938538No Hit
CTCATAAGCAATCCTTGTTTTTCTTCCATTCTCACCCCTCCAGAAGTTTC6650.2017052434263806No Hit
GCTTATGAGAGAATGTGCAACATTCTCAAAGGGAAATTTCAAACAGCTGC6130.18593280333890425No Hit
CATTATGATCTGGCATTCCAATTTGAATGATACAACTTACCAGAGGACAA6100.18502285487231906No Hit
TCCCAACACTGTGTCAAGCTTTCAGGTAGATTGCTTCCTTTGGCATGTCC5770.17501342173988213No Hit
CCCATATACAAGAGAGTAGATGGAAAGTGGATGAGGGAACTTGTCCTTTA5380.16318409167427483No Hit
GCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCATTCTGCTGT5370.16288077551874647No Hit
CATATTAAGAGGGTCAGTTGCTCACAAGTCCTGCCTGCCTGCCTGTGTGT5160.15651113625265023No Hit
GCATAAATCTCATAGGCAAATGGTGACAACAACCAATCCATTAATAAGAC5040.1528713423863095No Hit
TGTCAAAGGAGGGCACGATCGGGTTCGCTGCCCTTTCGTCCGAGAGCTCG4990.15135476160866757No Hit
CTATGGTATCAAGTACTCTTGAACTGAGAAGCAGGTACTGGGCCATAAGG4790.14528843849809972No Hit
CGTTTAAGGAGACGATAAATGCATTTGAAAAAAAGGCGATCAAGAATCCA4560.13831216692094672No Hit
CATTAATACTGCTTTGCTTAATGCATCCTGTGCAGCAATGGATGATTTCC4550.13800885076541833No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAAAACGTTCTTA4540.1377055346098899No Hit
ATACCATAGTATCCATGTTTTCATTTGAAGCAATTTGTACTCCTCTAGTG4390.13315579227696406No Hit
GTCGTACTCCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCA4130.12526957223322585No Hit
GTCATAAGTTCCATTTTTCACACTTTCCATGCATTCATTGTTACACTTGT4110.12466293992216908No Hit
GCATAGATCTCATAGGCAAATGGTGACAACAACCAATCCATTAATAAGAC4100.12435962376664067No Hit
CTCTAGCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCATTCT3990.12102314605582838No Hit
CTCATAGACTCTGGTACTCCTTCCGTAGAAGGCCCTCTTTTCAGACCGCG3990.12102314605582838No Hit
ATATAATGCAGAATTGTTGGTTCTACTGGAAAATGAAAGGACTTTGGATT3970.12041651374477158No Hit
ATTCACAGCTGTGGGCAAAGAATTCAACAAATTAGAAAGAAGGATGGAAA3840.11647340372290249No Hit
CTCTAATATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAA3780.11465350678973214No Hit
CCCTAGGAGATCTGGAGCTGCTGGCGCTGCAGTCAAAGGAGTTGGAACAA3710.1125302937010334No Hit
ATATACAACAGGATGGGGGCTGTGACCACCGAATCGGCATTTGGCCTAAT3710.1125302937010334No Hit
CTTTTCAGACCGCGTTTAAGGAGACGATAAATGCATTTGAAAAAAAGGCG3580.10858718367916431No Hit
GATCTATGCTGGGAGTCAGCAATCTGTTCACAGGTTGCGCATATTAGGCC3510.10646397059046556No Hit
TTGTTACACTTGTGGTAGAATTCAAAACACCCGTTTCCTATTTCTTTGGC3340.10130759594648289No Hit
ATCATAATGTGAGTCAAACCAGCCGTTGCATCATCACCATTGTTGGCTTG3310.10039764747989773No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGAGGT100.0070843548145.02989145
TCGGTCC100.0070843548145.02989145
AGCGAAA301.09572284E-7120.913281
GGGTAGA650.0100.4510351
AAGGTAC259.3847525E-486.991539
GTAGAAA2550.079.660511
TTAGTAG406.634787E-572.547961
AATAACC406.639779E-572.536963
AATTATA651.3902354E-766.957195
CCATAGG451.1932499E-464.438188
CCAGCGG350.003557193362.1745345
ATCCCGT1700.059.718193145
CAAGGGT2050.056.579868
TACAAGC400.00603512154.4027142
TAGAAAC3900.053.9377372
GTCAATT953.1182935E-853.4482842
CTATCAA553.207749E-452.754153
TAGATAA1650.052.754154
TCTCCGC553.2125606E-452.738144145
TAGATAT858.8751403E-751.2025573
Produced by FastQC (version 0.11.5)