FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n01_S1_R1_D09_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n01_S1_R1_D09_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences102
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCCTTCCTCCACTCCCCTTGGTGACTCTCCGATTGGAACTGTTTCCGC10.9803921568627451No Hit
TGCAACTACACATTCCTGGATTCCCAAGAGGAATCGTTCTATTCTCAACA10.9803921568627451No Hit
GATGTCAATCCGACTCTACATTTCCTAAAAATTCCAGCGCAAAATGCCAT10.9803921568627451No Hit
GTCAATTGCTGAGGCAATAATTGTGGCCATGGTATTCTCACAGGAGGATT10.9803921568627451No Hit
ATCCGCAAGCGATTTGCAGACAATGGATTGGGTGATGCCCCACTCCTTGA10.9803921568627451No Hit
GATGAATACTCCAGCACGGAGAGAGTGGTAGTGAGTATTGACAGATTTTT10.9803921568627451No Hit
GCCGGACCACAGTCCAAGAAAGGCGGAACCAATCTGAGCTCAAGTTTCCT10.9803921568627451No Hit
GTACCCTTCCCTTTCAAAGTCATGCCCACTTGCTACTGCAAGCCCATACA10.9803921568627451No Hit
TCTTTTCCAGAGGTCTGACCTGGGAGTATCCAAAGTGCCAAATTTCCTTC10.9803921568627451No Hit
CAATAAGGCATGTGAATTGACTGATTCAAGCTGGATAGAACTTGATGAAA10.9803921568627451No Hit
GCAGCTAGCCATAAGGGGGAGAGACCGGGAAAGTCAGAGGCACATGGAGA10.9803921568627451No Hit
CAGGTAGTCTGAGGGGGCGTGGTGTCGTCCTCAAGACTGGTTCATTTTTG10.9803921568627451No Hit
GTCTGACAGAGAAAGCGAGAGAGGAACACCAGCTGGGAGAGTGGGAGAGC10.9803921568627451No Hit
CTACAGGACCTGCAAGTTAGTGGGAATCAACATGAGCAAAAAGAAGTCCT10.9803921568627451No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA10.9803921568627451No Hit
GCTCTTATTAGCTAGCCTCTCTTATTCAGTCTTTGTTTTTTCTTCCCTAT10.9803921568627451No Hit
ATACTGACTCATCACAGTCATGAAGCATGATCAAAATGGGAGATAATGGA10.9803921568627451No Hit
CTTTAATACAATAAAAAAAAAAAAAAGTGTGCGGGGACAGGCTGCCCTCC10.9803921568627451No Hit
ATACTGACATCAGAGTCACAGCTGGCAATACCAAACGAGAAGAAAGAAGA10.9803921568627451No Hit
GTAATTGAGTTATCTTTTATTGCTTATTTGGTCACCACTTCCTCTCTGCC10.9803921568627451No Hit
CCCCGCGCCGGGTGCCCCCCAGCTCCCCCCGCGTGCTGGACCCCTGCCGG10.9803921568627451No Hit
CCTTGGAGCCAAGTAAGTGCTGGCGTGCAGGGCAGGGCTTCCACCTGGCT10.9803921568627451No Hit
GTGAGTGAGAACTCCATACTTACAAAGTTCACCACATCAGTATCATTTCT10.9803921568627451No Hit
CCATACGGCCATAATAAAAAAGTACACATCAGGAAGGCAAGAGAAGAACC10.9803921568627451No Hit
GCACTTAACCCAAGGGACGTGCTGGGAGCAGATGTACACTCCAGGAGGAG10.9803921568627451No Hit
GTGAAATGGAGGTAATGGCTCTTATCACCATAGATTTATTGTCAAGATCA10.9803921568627451No Hit
GGCGATCTGAAAGTTTTGCTAGACTCCTCACACAACCTGTAAACTGACCC10.9803921568627451No Hit
CTTCCAAATGTGTGCATATTGCAGCAAACTTGTTAGTTTCGATTTTCGGA10.9803921568627451No Hit
CATGGTACCTTCGGCCCTGTCCACTTCAGAAATCAAGTTAAAATAAGGAG10.9803921568627451No Hit
GCTAAAGACAAGACCAATCTTGTCACCTCAGACTAAGGGAATTTTAGGAT10.9803921568627451No Hit
ATGTACAATTTAGTATGTGTCTGCTCGCTTAATTAACATCTACATCCAAC10.9803921568627451No Hit
CAATTATGCTAGCCCATTGTTTCGTAAAGGTTAACACATCTTGCAATGTT10.9803921568627451No Hit
CTCCAGGCGCTGCTGCACCAGGCGGGAATAGAGGTCCTGGCCCTCCATGA10.9803921568627451No Hit
GGCGGGAATAGAGGTCCTGGCCCTCCATGCGCTCTGAGTGCTCCCGCAGC10.9803921568627451No Hit
GCGGAAAAGGCAATGAAAGAATATGGGGAAGATCCGAAAATCGAAACTAA10.9803921568627451No Hit
GAGCATGTCTGCTTTTACAACTCTTGTCAGAGGCTAAGACACTCTACGGA10.9803921568627451No Hit
CATGAGAATAATGGCCATGATTACAGAGAGGAATGAACAAGGACAAACCA10.9803921568627451No Hit
GTTGGAATCCATGAAGAAAATGCCCTCATACTGCAGCTTCTGTTTCCCTG10.9803921568627451No Hit
GTCCTAAACTGTAAAGGCCTAGCTGATTCCCATGGTACCATATTTCTCCA10.9803921568627451No Hit
TGTTTCCGATTTGTTTTCAAAGAATTCCTTGGTCATGTCTTTCTCTTTGA10.9803921568627451No Hit
AACCCATACAGGTTTGTTTTCCGTCTTCCTTCTTTGGTCCTACATTTGCT10.9803921568627451No Hit
GTGTTGTCCCCAGTGATTGTGAAAGAAATCTCTGTGTCTTGTGAATTAGT10.9803921568627451No Hit
TTTTAGGACAGGAGACAAAGCATGAAACTTGGTTTTGCGATAAAGCAGTG10.9803921568627451No Hit
GGACACCTCCGTGAGCCATCCTGGCCCCTCGGGTTGGGAATTGAGCGCCG10.9803921568627451No Hit
GTCTAACAGGATAACGCTGATGTGTCCTTACTTACTGAGGGATTTCTCCT10.9803921568627451No Hit
GAATGGCATGATGTGTATCAAAGTTGAAGCTGTATGATGGATACACGGAG10.9803921568627451No Hit
GATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCCAATGATCGTC10.9803921568627451No Hit
GTAGAGGTCCTCCACTGTATCGGAACCCTTCTTGATGTTCTCTTGGACCT10.9803921568627451No Hit
GCTTACATTGCCTCTTGTGAGTGTATCAAATCTCTAGAACTTTCTCATCT10.9803921568627451No Hit
ACTTTATATGCTTTGTACACTTAGGATGATTGTTAATGTTTTCCTATTAC10.9803921568627451No Hit
GTCCAACCCTGACCTACTGAACCAAACACTGGGAGCATCGCCCAGCACTC10.9803921568627451No Hit
TCCCAGAGATCACTATCTCTCCAATCACACCAAACCTCAGATTTGACCAA10.9803921568627451No Hit
CTTCAATACTTAATTTCAGAGCATCCATCAGCAGGAACTTTGACCGCTGA10.9803921568627451No Hit
GGCCTCCACCATGCTAGAAATTCCAACCGGTCTCCTATATGAACTGCTAG10.9803921568627451No Hit
TTTATATGCACCCCAGAGAGAAAGGCTACCCACTAAAGCGTGGGGCATAT10.9803921568627451No Hit
GCATCACTGAGTCCTGGGATGATGATGGTCATGTTAAACATGCTAAGTAC10.9803921568627451No Hit
CCTGAGGAGGTAGAGTCTGAGGACAGTTTTTTCGCATCCTCAGGGCGCCA10.9803921568627451No Hit
CGTAGAGACCAGGCATTTTTTCCTGAAGGAACTGGTAATTTCATTATTTT10.9803921568627451No Hit
TTCCAGAACTGGGGAATTGAATCCATCGACAATGTGATGGGAATGATCGG10.9803921568627451No Hit
AGCAGGTACTGATCCAAAATGGAAGAGTTTGTGCGACAATGCTTCAATCC10.9803921568627451No Hit
GCATGAGGCAGACCCTTAAGGTCATCGTAGGTTCCGTGGGTGGAGGTTGC10.9803921568627451No Hit
CTTAATTTCAGAGCATCCATCAGCAGGAACTTTGACCGCTGATGGCAAAG10.9803921568627451No Hit
GAATAGAACGATTCCTCTTGGGAATCCAGGAATGTGTAGTTGCAACGGCA10.9803921568627451No Hit
TAACAAAAGAGAAGAAAGAAGAGCTCCAGGATTGTAAAATTGCTCCCTTG10.9803921568627451No Hit
TGCCCAATTAGCACATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTC10.9803921568627451No Hit
AAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCAC10.9803921568627451No Hit
CTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGG10.9803921568627451No Hit
GAAAAAAGTCGCAGTCAAGCAATCTATACATAGTCTCTATCAACAGTTTC10.9803921568627451No Hit
AGGCAAGATCATTGTGGGCTGGAGTTAGGGAGGCTCCCAGGAGGAGCTGG10.9803921568627451No Hit
ATTTAAATATGACAGGGACATTTGAATTCACAAGCTTTTTTTATGGCTAT10.9803921568627451No Hit
AGTTAAAATAAGGAGGAGAGTTGATACAAACCCTGGCCATGCAGATCTCA10.9803921568627451No Hit
CTGGGGCACATTTCCAAGAGAGATGCTAATGTGTCTGCTGACACTGCTGC10.9803921568627451No Hit
CACATGGAGTTCCTTTAGGTGGCCAGTCAGGTGCTGTGTCCCACATACCC10.9803921568627451No Hit
ATTTTACAATCCTGGAGCTCTTCTTTCTTCTCTTTTGTTATTGCCAGCTG10.9803921568627451No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCTCA10.9803921568627451No Hit
ATGATAAGGATCAAAGCCCAACTGTAATGTGCTTAGGAAAGGGGAAGAGC10.9803921568627451No Hit
GGCTGGACCGGTCCGGGTCCCCTGGGGAGCGGCGGTAGGCAGGGGTTTTA10.9803921568627451No Hit
TTCCCTACATTGTTAGTGCTGTGCTCTGGGGTAGGCTGGCCAAAGGGCAG10.9803921568627451No Hit
CATTTACTCCAGACACTCCAAAGCTGGGTAGCTCCATGCTAAAATTAGCC10.9803921568627451No Hit
ATTTTCACCGAGTGCCCCCTTCAATTGGCTTGTTCTCTTCATGTTCTTTG10.9803921568627451No Hit
ACTCTACTTTTCCTAAAAATTCCAGCGCAAAATGCCATAAGCACCACATT10.9803921568627451No Hit
GGCATCAATCCGGGCCCTAGACACCATGGCCTCCCCCATGCTAGAAATTA10.9803921568627451No Hit
TCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTATGCTAGAGTCC10.9803921568627451No Hit
GGACAGGGCCGAAGGTACCATGTTTCAACCTTTCGCCCTTTTCGAAATAA10.9803921568627451No Hit
GTGAAGGAACCCCAAAGTGTCTTAGGACATTTAACTAAGCCGACCAAGAA10.9803921568627451No Hit
CTTAAAAATAGGACAATACTCACTACCACTCTCTCCGTGCTGGATTATTC10.9803921568627451No Hit
CTACTGGGAGCCCAGACTGTTCAAGCTTTTCGCAAATGCTCCTAGCTAAA10.9803921568627451No Hit
GCGTCATATAGCCCCCCAAGATCGAAGGTTCCAGGTTCCAGGTTGTCCCT10.9803921568627451No Hit
ACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG10.9803921568627451No Hit
GCTCAATGCATCCTGTGCAGCCATGGATGACTTTCAGCTGATCCCAATGA10.9803921568627451No Hit
TGCTGGAACAGTGTCCTTACGAATCCACTGTACCGGCTTCTGGTTGCCTT10.9803921568627451No Hit
CCCTAAGGCAACCAGAAGCCGGTACAGTGGATTCGTAAGGACACTGTTCC10.9803921568627451No Hit
GTTTAGAGAAGCTGGAAGGTGATAACCAGCACAGGGAAAGTCGCTGTGTC10.9803921568627451No Hit
GGGAGTGGCCGTGGGCGCTGACGGCACCCGGGTCTGGGGGGGTGTTGTGG10.9803921568627451No Hit
GAGTGATGGTAGGTGGAGTCTGCTGTCCTGAGAGGATTTCTCATTTTCGG10.9803921568627451No Hit
GCAAACTCTGCAGGGCCTCTCGGAGAACGCCAGTTTCCCCCAACCCGTAC10.9803921568627451No Hit
ATCTTTGTCAATCAGGAAAAACCACTTCATAGGATTTCTGTGAGGATCAA10.9803921568627451No Hit
GCCCTGACTTTTCTCTCTGCCCATGATAGCCAAGCGAGGCTAAGTCCAGA10.9803921568627451No Hit
CCTCCAAACACTGAAAATAAGAGTACATGAAGGGTATGAAGAATTCACAA10.9803921568627451No Hit
CTGCCCGGGCCTTGGGCTCCAGCTCCCCACCAGCTGCCCCCACTTCCTGC10.9803921568627451No Hit
CCCTTGTCCTGAATTCTCCTCCCGGCAGGACCCCCAAAGCAGATCACCTT10.9803921568627451No Hit
GATTCCAAGAGGAAAGCTTTCCACAAGAGGGGTCCAGATTGCTTCAAATG10.9803921568627451No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)