FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n01_S1_R1_D07-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n01_S1_R1_D07-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences939920
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG15010.15969444208017702No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA13810.14692739807643204No Hit
TCCTTAGACAGAATCCAACTGAGGAACAAGCCGTAGACATATGCAAGGCA12410.1320325134053962No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA12400.13192612137203166No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA11980.12745765597072092No Hit
GTCTAAGGATGTCCACCATCCTTACTCCTCCAATCTGTGTGCTGTGGCAC11530.12267001446931652No Hit
ATATAAACACTGCCTGTTCCGCCGGCTACTGGGAGAAACCTTGTTTTACG11490.12224444633585838No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT11130.11841433313473487No Hit
GAATAAGAGAGGCTATCTAATAAGAGCACTGACATTAAATACGATGACCA10440.1110732828325815No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC9500.10107243169631458No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG9490.10096603966295004No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTAGAA5150.054.9160081
AGCGAAA1850.054.877951
CGAAAGC1850.054.872113
CAATTCG5200.051.5851179
GACAGTA2550.048.3327
GTAGAAA8000.048.0427061
ACTATCC803.735088E-545.3112568
AGCGAGC1851.8189894E-1243.1138145
TATATTC5800.042.505613
CCTTATA5750.041.6141932
GATCTAC700.001055369441.4384541
ATATTCA6400.038.5207144
TTATACT6200.037.4242974
GCGAAAG2750.036.9139672
ACAATTC7900.035.7901548
TAGATAT1656.0092134E-835.156163
TTTAAAC8900.033.403293
AATATAT7050.032.915651
GTCTAAG6200.032.7497441
CCCTTAT6950.031.3024311
Produced by FastQC (version 0.11.5)