FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n01_S1_R1_D05-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n01_S1_R1_D05-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences997334
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG17010.170554698827073No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA16410.1645386600677406No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA14330.14368305903538833No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA11590.11620981536777047No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG10270.10297453009723925No Hit
GGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATG10140.10167105503271721No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA2400.063.4432451
TATACGT1056.9016096E-848.3377154
CCTTATA6700.045.4518782
CGAAAGC3400.044.7834663
CGTTACG657.3333003E-444.6194272
CAATTCG5650.043.62799
GCGAAAG4250.039.238852
TATATTC8300.038.4372183
TACGGGT800.002035706536.2532845
GACAGTA4250.034.1173177
TATACTG10050.033.9085465
TTATACT9450.033.7596744
TAGAAAC10800.033.5678563
GTAGAAA10250.033.2469182
AGTAGAA9400.032.3965531
ATATTCA9400.031.6252044
ACAATTC8700.030.8330358
ACGTTAC950.004725195530.529081
GGTCGTT4450.029.3255567
AATATAT10100.027.9975851
Produced by FastQC (version 0.11.5)