FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n01_S1_R1_D01_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n01_S1_R1_D01_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA24.545454545454546No Hit
GGCGCTAGTTATGCGGATGTCACAAATGCAGTGGGGGTCCTCCTCGGAGG12.272727272727273No Hit
GGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATG12.272727272727273No Hit
GCAGTGGGACACTTCTGCTGTAAAATAGTTCCTCCTCATGCTTGCGATAT12.272727272727273No Hit
AATATATTCAAGAAGTAGATAATAAAAGAAATGAGAGATCTAATGTCGAA12.272727272727273No Hit
GGCCAAGATGGCTCGTAAATTGTTCATCAAATAAGACAGATACTCATATA12.272727272727273No Hit
CTGCCGGCCCATGACCACGCTCCTGCCGGCGGCCCAGCATACCCGCCCCG12.272727272727273No Hit
GAATAAAAGAACTAAGAAATCTAATGTCGCAGTCTCGCACCCGCGAGCTA12.272727272727273No Hit
GTCACGCAAAGAACAATAGGGAAGAAAAAACAAAGACTGAATAAGAGAGG12.272727272727273No Hit
CCGTAGACATATGCAAGGCAGCAATAGGGTTGAGGATTAGCTCATCTTTC12.272727272727273No Hit
GTATGTGAGGTTGAGAGGTAGAATATGTGTTTATTTTAATTACATGCCAT12.272727272727273No Hit
GGCCAAGCGGATCCTCAGTCAAGAAAGCAGAAGAAGTGCTACCGTGCCAC12.272727272727273No Hit
GAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGCTACTCACTAAGACCA12.272727272727273No Hit
ACTCCATACTTACAAAGTTCACCACATCAGTATCATTTCTCAAATGAGAC12.272727272727273No Hit
GTTCATTGATGCTTAATGCTGGGCCATATCTCTTGTCTTCTTTGCCCAAA12.272727272727273No Hit
GTTGAGAATAGAACGATTCCTCTTGGGAATCCAGGAATGTGTAGTTGCAA12.272727272727273No Hit
TATCGCAACACCTGGGATGCAGATTAGAGGTTTCGTATACTTTGTTGAAA12.272727272727273No Hit
ATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACT12.272727272727273No Hit
GAGTGGGAGTGAGTAGTGCCCGATGTTTAAGGGTTAGAGATCACAGCGGG12.272727272727273No Hit
GTCTTGGGAGTCTCGATACTGAATCTTGGACAAAAGAAATACACCAAGAC12.272727272727273No Hit
AGCAAATGAAGGACCAAAGAAGGAAGACGGAAAACAAACCGGTATCGGTT12.272727272727273No Hit
CCTGGAGTGAACCTCTGCTCTCAGCACGTCCCGAGGCTTAAGTGGATGAC12.272727272727273No Hit
GGGCCAGCTTTGAGCAATGTTCATTCCCAAACAGAACCCATATTATATGG12.272727272727273No Hit
TTATTTGGACAGTGTCAAATGTCCCAATCTCATCCCGCATTTGCTGGCAC12.272727272727273No Hit
TCCCAGGTTTGTATATAGACCGTCTGGTGCAATGGAAGATATAGGCCGCG12.272727272727273No Hit
GGTTTGTATCAAATCTAATCCTTATTTTAACTTGATTTCTGAAGTGTACA12.272727272727273No Hit
GTCTATATGCGACTCCACAACTTCAGGGGTTATCGGCAGAATCTAGAAAA12.272727272727273No Hit
CCTAAAAATTCCAGCGCAAAATGCCATAAGCACCACATTCCCTTATACTG12.272727272727273No Hit
GGTCAAAATTATTCAATATGGAGATAATAAAAGAACTGAGAGATCTAATG12.272727272727273No Hit
GAATAATGGACATGCCCAGCATTAAGCATCAATGAACTGAGCAATCTTGC12.272727272727273No Hit
GAGAGCATTCCTCTAATTAAAGGCTGCATAAGATATAAAATGAGGAAATG12.272727272727273No Hit
CATGAAGAGAAAAACACAATTCAAGTGGGCACTCGGTCAAAATAACGCAA12.272727272727273No Hit
GGACAAAGAACATGAAGAGAACAAGCCAATTGAAGTGGGCACTCGGTGAA12.272727272727273No Hit
GACCCGAGACTGGAGCCACACAAATGGGAAAAATACTGTGTTCTTGAAAT12.272727272727273No Hit
GACAAAGTAATGGACAGGCCTCATACAAGATCTTCAGAATAGAAAAGGGA12.272727272727273No Hit
CACTTAGTGTTGTCCCCAGTGATTGTGAAAGAAATCTCTGTGTCTTGTGA12.272727272727273No Hit
CCCCTAACTGCCTTGATCATGCAATCGTCCTGTGAGAATACAATCGCCAC12.272727272727273No Hit
ATATAAATAAGACAGGGACATTTGAATTCACAAGCTTTTTTTATCGCTAT12.272727272727273No Hit
ACCTTGGTTTACGGCCCACGTCTCTTTAGAGCATGTCCGCCACCATCAAG12.272727272727273No Hit
ACATATAGGTGCCATAGGGGAGACACACAAAGACATACGATCAGATAATT12.272727272727273No Hit
GGCTGGGGAGTCTCGAGACTGAATCTTGGACAAAAGAAATACACCAAGAC12.272727272727273No Hit
CGTTTCAACACTCAAGAAATTTAGTTAATGGAGTAAACTTTTTATCAGTA12.272727272727273No Hit
GCAAAACCCAGGGATCATTAATCAGGCACTCCTCGATTGCTTCATATAGC12.272727272727273No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)