FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n01_S1_R1_D01-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n01_S1_R1_D01-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1117155
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTATATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATGTCGCA21450.19200558561703612No Hit
TTTTTAAACTATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG19640.17580371568851233No Hit
ATGTAATACCTATCAAAATACCAATAACACTTCTCACAGAACTAGAACAA14920.1335535355434116No Hit
GTATAGACATCTTAACAATATTGTTCTTCCAATCCATGAGCATGGATTGT13110.11735166561488782No Hit
GTATTACATCAAATATGTAGATTTTGGGTAGTATAGACATCTTAACAATA12860.11511383827669391No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT12250.10965353957150081No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA3900.046.4870571
CTATTCG7050.043.1897549
TATATTC11800.041.7892073
CGAAAGC4400.041.202593
CGTGCTA2350.040.1047748
ATTATAT11450.036.734931
GCGAATG1803.1959644E-936.25504145
TCAATAT13700.033.8672528
ATATTCA13700.033.347034
CAATATG13500.033.294969
CTAGTAC2404.3655746E-1133.236763
TATTCAA14750.031.4648155
GTAGAAA13900.031.3035161
ACCATCG3250.031.2295158
TATACGT2807.2759576E-1231.0785274
TAGAAAC13200.030.7646032
TTAAACT11850.029.3737964
AGTAGAA12000.029.0079231
GGTCGTT4000.028.9988357
TACCTAT9950.028.4159437
Produced by FastQC (version 0.11.5)