FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n01_S1_R1_C09_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n01_S1_R1_C09_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences85
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATGGATTTGTGGCTAATTTTAGCATGGAGCTACCCAGCTTTGGAGTGT22.3529411764705883No Hit
ATTACAAAGAGAACCGGTTCATTGAAATTGGAGTAACACGGAGGGAAGTC11.1764705882352942No Hit
GAGCCGAACGGCTGCATTGAGGGCAAGCTTTCCCAAATGTCAAAAGAAGT11.1764705882352942No Hit
AATCCATCTACATAGGCTCTAAAGTTTTCAAGGCTGGGGAAGTTCGGTGG11.1764705882352942No Hit
GTCAATTGCTGAGGCAATAATTGTGGCCATGGTTTTCTCACAGGAGGATT11.1764705882352942No Hit
GTCTCTTGTCACTAAGGCAACCAGATGCGGGTACAGTGCATTCGTAAGGA11.1764705882352942No Hit
GGATGTTTCTGAACCACTCGGGTTGATTTCTGGTGATATATGTAATCATC11.1764705882352942No Hit
ATGGAAGACTTTGTGCGACAATGCTTCAATCCAATGATCGTCGAGCTTGC11.1764705882352942No Hit
CCTCTGAATTGTCTCCGAAGAAATTACATGGTTCATTACTCATCTCAAAC11.1764705882352942No Hit
CTCCTTTCACCGCAGCACCTGCGGCACCAGACCTTCGGGGAAGTGTTGCA11.1764705882352942No Hit
GAAAGAAATCTCTGTGTCTTGTGAATTAGTCATCATCTTTCTCACAACAT11.1764705882352942No Hit
CATTAAAGACGATGACCAAAGCTGCAGAGAGAGGCAAGTTAAAAAGAAGG11.1764705882352942No Hit
TGATTACATATATCACCAGAAATCAACCCGAGTGGTTCAGAAACATCCTG11.1764705882352942No Hit
GAAGAAAGAAGAGCTCCAGGATTGTAAAATTGCTCCCTTGATGGTGGCGT11.1764705882352942No Hit
GACTAATCCAGAATAGCATAACAATAGAGAGGATGCTGCTTTCTTCTTTT11.1764705882352942No Hit
TATAAACACTGCCTGTTCCGCCGGCTACTGGGAGAAACCTTGTTTTACGG11.1764705882352942No Hit
ACTCACTACCACTATCTCCGTGATGGAGTATTCATATTCTCACCTTTTGC11.1764705882352942No Hit
TCCTTTAAGAAATCTATTAGCCTTCCTGACTCATTAGCTGTTAGGCCATT11.1764705882352942No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT11.1764705882352942No Hit
CCACAGCACTCTGCTGTTCCTGTTGATATTCTTCCCTCATGGACTCAGGC11.1764705882352942No Hit
ATATAGCCCCCCAAGATCGAAGGTTCCAGGTTCCAGGTTGTCCCTAAGTG11.1764705882352942No Hit
CACCCTTGGCCTCGATATCCAAACAGCCACTCTTGTTGGGAAACAAATAG11.1764705882352942No Hit
TTCCTATTCCACCATGTTACGGCCAGAGGTGATACCATCACTCGGTCTGA11.1764705882352942No Hit
GAATAAGAGAGGCTATCTAATAAGAGCACTGACATTAAATACGATGACCA11.1764705882352942No Hit
CTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGA11.1764705882352942No Hit
GTGCGACAATGCTTCAATCCAATGATCGTCGAGCTTGCGGGAAAGGCAAT11.1764705882352942No Hit
GTTCATTGATGATTAATGCTGGGCCATATATCTTGTCTTCTTTGACCAAA11.1764705882352942No Hit
GAGAATTGCCTCTTACCAGTATCCTCAACCCTGATCCTCTCACATTCACA11.1764705882352942No Hit
GCAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCCCACTCGCG11.1764705882352942No Hit
ATCCAATGATCGTCGACCTTGCGGGAAAGGCAATGAAAGAATATGTGGAA11.1764705882352942No Hit
GTATAAGTTTGGACCTCCATCTGATACTAATAGCCATACCTTTGATTGGG11.1764705882352942No Hit
ATTTGCAGACAATGGATTGGGTGATGCCCCATTCCTTGATCGGCTCCGCC11.1764705882352942No Hit
GACCAAAGAAGGAAGACGGTAAACAAACCTGTATGGGTTCATTATAAAAG11.1764705882352942No Hit
AGATTAGAGGTTTCGTATACTTTGTTGAAACTTTAGCTAGGAGCATTTGC11.1764705882352942No Hit
CTATTTCTTCTCTGTTTAATTTTGCTTCCTCTGAGTATTTTGGGTAGTCA11.1764705882352942No Hit
GTCCCAAGCACATCCCGCATTTGCTGGAACAGTGTCCTTACGAATCCACT11.1764705882352942No Hit
GCTTTTGAGTGTCTGGAGTAAATGAATCAGCTGACATGAGTATTGGAGTA11.1764705882352942No Hit
TCTGTAGTCTTTGATGAACAATTGAAGCGCCATCTGGGCCGTTGCCTGTC11.1764705882352942No Hit
CCTCAATGCAGCCGTTCGGCTCGAATCCATCTACATAGGCTCTAAAGTTT11.1764705882352942No Hit
TGCTGGGAGCAGATGTACAATCCAGGAGGAGAAGAGAGAAATGATGATCT11.1764705882352942No Hit
ATTTTAAGCAGACTTCAGGAATGTGAAGAATCCGGATATTGTATAAGTTT11.1764705882352942No Hit
CTCTCGAACATGTACCCTTTCCCTAGTCTTGGCATTTTGTTTGAGAACAT11.1764705882352942No Hit
GGCGGATACTAATAGCCCTACCTTTGATTGGGTTTGATCCCCCAGCGTCT11.1764705882352942No Hit
ATACTGATGTGGTGAACTTTGTAAGTATGGAGTTCTCACTCACTGACCCG11.1764705882352942No Hit
GTTTCTAGCAGCGCTAATCAAACTTTGGTCAACATCATCATTTCTCTGTT11.1764705882352942No Hit
GAGTAGGATGCAATTTTCCTCATTGACTGTGAATGTGAGAGGATCAGGGT11.1764705882352942No Hit
GGGCAGTATTCCGATCATTCCCATCACATTGTCGATGGATTCAATTCCCC11.1764705882352942No Hit
TTATAGGTGCCATGCTCAGGATGTTTCTGAACCACTCGGGTTGATTTCTG11.1764705882352942No Hit
CAAAACAATAGCGATAAAAAAAGCTTGTGAATTCAAATGTCCCTGTCTTA11.1764705882352942No Hit
GTATACGAAACCTCTAATCTGCATCCCAGGTGTTGCGATAGCCCTTCTTT11.1764705882352942No Hit
GATTGCAGCACTTCTGGTACATCTGTTCATCCTCAAGAATTCCCAGTTGG11.1764705882352942No Hit
GCCTTATTTTCTCAATTTTCTTCTTTGTTGATTCATTGAAGTACTTCAGG11.1764705882352942No Hit
GGAACTGGGAAATTGTGAAAATTCAATGGTCACAAGCTCCCACAATGTTA11.1764705882352942No Hit
ACATGACCAAGAAGATGGTCACGCAAAGAACAATAGGGAAGAAAAAACAA11.1764705882352942No Hit
GAGGCCCATCAGGCAATCTGAGGGGGCGTGGTGTCGTCCTCAAGAATGGT11.1764705882352942No Hit
CGCCAGGAACATTCGAGGATTTTGATTTTCATTCCACTTAGTGTTGTCCC11.1764705882352942No Hit
AGTGAATATTGACCGATTTTTAAGGGTTAGAGATCAAAGAGGGAGAGTAC11.1764705882352942No Hit
GCTCTATCCATGATGTTCGGGTCCCCATTCCCATTTAGGGCATTTTGGAC11.1764705882352942No Hit
AACTTCCCCAGCCTTGAAAACTTTAGAGCCTATGTAGATGGATTCGAGCC11.1764705882352942No Hit
GTTGTATAGCATTGTGGGATCTTGTGACCATTGGATTTTCACAATTTCCC11.1764705882352942No Hit
TTACATATATCACCAGAAATCAACCCGAGTGGTTCAGAAACATCCTGAGC11.1764705882352942No Hit
CAAATGCTCCTAGCTAAAGTTTCAACAAAGTATACTAAACCTCTAATCTG11.1764705882352942No Hit
TAAATAAGCTGAAACGAGAAAGCTCTTATCTCTTGTTCTACTTCAAGCAG11.1764705882352942No Hit
GTTAGGGGCGATCTGAACTTTGTCAATAGGGCAAACCAGCGACTGAACCC11.1764705882352942No Hit
GTCTAAGGATGTCCACCATCCTTACTCCTCCAATCTGTGTGCTGTGGCAC11.1764705882352942No Hit
CCCAATTACAGCAGACAAGAGAATAATGGACATGATTCCAGAGAGGAATG11.1764705882352942No Hit
GCATCTTTGGTCATCGTATTTAATGTCAGTGCTCTTATTAGAGAGCCTCG11.1764705882352942No Hit
TTCTTTAACTGTTGTGCAGTGTGCTCGCGAAGATAGGGACAGTACGATGT11.1764705882352942No Hit
GCTTGGGGTCATGTCGGGCAGTATTCCGATCATTCCCATCACATTGTCGA11.1764705882352942No Hit
CCCATATAGGTGCCATAGGGGAGACACACAAATTCAGACGAGAAGATCAT11.1764705882352942No Hit
CGTCAATGGCATTCTGTGTGCTCTTCAGGTCGGCTGCATATCCTGACCCC11.1764705882352942No Hit
TCATATATGTAATCATCGCCAGGAACATTCGAGGAATTTGAATATCATTC11.1764705882352942No Hit
GTGCAGCCATGGATGACTTTCAGCTGCTCCCAATGATAAGCAAATGTAGG11.1764705882352942No Hit
GCCCGCATGGCCTCCGGCCCGCCTGCCGGCGCCGGCCCGCGTTGCACACC11.1764705882352942No Hit
AAAAAAGACAGGGACATTTGAATTCACAAGCTTTTTTTATCGCTATGGAT11.1764705882352942No Hit
GAAACAGAAAACATTAACAAGAAGATATACACTAAAACAACAATAGTTAA11.1764705882352942No Hit
AGGGAATACCACTATATGATGCAATCAAATGCATGAAGACATTCTTTGGC11.1764705882352942No Hit
CCTCTGACTAAGGGAATTTTAGGATTTGTGTTCACGCTCCCCGTGCCCAG11.1764705882352942No Hit
CTCCAGCACGGAGAGAGTGGTAGTGGGTATTGACCGATTTTTAAGGGTTA11.1764705882352942No Hit
ATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTG11.1764705882352942No Hit
TATAACTTCTGTTCGCATGTCGGATGTCCGTCCTTCATTGTTCCCGCTGA11.1764705882352942No Hit
GGGCAAACCAGCGACTGAACCCCATGAACCAACTCTTGAGGCATTTACAA11.1764705882352942No Hit
ATGGTATTTGTTCGAAACTATTATCTGTCGCTTTCAATCTGTGCCGCATT11.1764705882352942No Hit
GAGTAAGAGACAACATGACCAAGAAGATGGTCACGCAAAGAACAATAGGG11.1764705882352942No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATATCA50.0145.06
TTACATA50.0145.01
TACATAT50.0145.02
ACATATA50.0145.03
TATCACC50.0145.08
ATCACCA50.0145.09
ATATCAC50.0145.07
CATATAT50.0145.04
ATATATC50.0145.05
CAGAAAT100.072.5145
Produced by FastQC (version 0.11.5)