FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n01_S1_R1_C08-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n01_S1_R1_C08-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences865594
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA2650.049.2546041
CGAAAGC3750.034.8045773
GTACTAC900.00362460332.228321
GCGAAAG4650.029.627552
ACCGTCC2802.6375346E-1028.4809518
TCCCTAT4600.025.2221641
TATAGAC3801.2732926E-1124.8058932
CCTTATA2552.7032966E-622.7480892
TATATAC3551.01292244E-720.425223
ATATACC3208.4573E-720.3933074
GTATACA3601.1732482E-720.14271
GTATAGA10300.019.712471
GTGTAAG4051.8768333E-819.6950861
CCCTATT5900.019.66362
GATATAC1850.00504250319.597173
TAGCGAA1850.005047559719.5937757
CTATATA4102.1624146E-819.4537754
CATAGCG1900.00588279119.0814575
CCTATTC5005.4023985E-1018.8524783
GTATAGG4253.263085E-818.768261
Produced by FastQC (version 0.11.5)