FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n01_S1_R1_C06-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n01_S1_R1_C06-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1062331
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATAAATCTCATAGGCAAATGGTGACAACAACCAATCCATTAATAAGAC31740.29877693487246443No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAGCTATCTCAATGCATGTGTT28040.263947865589915No Hit
GCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCATTCTGCTGT26340.2479453202438788No Hit
GATCTATGCTGGGAGTCAGCAATCTGTTCACAGGTTGCGCATATTAGGCC26090.24559200475181464No Hit
ATATACAAGAGAGTAGATGGAAAGTGGATGAGGGAACTTGTCCTTTATGA23410.22036446267688697No Hit
CTTAATATGAGTGCAGACCGTGCCAGAAAGGTGAGATCTTCGATCTCAGC21270.20022008206481784No Hit
GCATAGATCTCATAGGCAAATGGTGACAACAACCAATCCATTAATAAGAC20690.19476039012322902No Hit
GATTTATGCTGGGAGTCAGCAATCTGTTCACAGGTTGCGCATATTAGGCC18860.17753412072131944No Hit
CATTAATACTGCTTTGCTTAATGCATCCTGTGCAGCAATGGATGATTTCC18750.1764986619048112No Hit
CTCTAGCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCATTCT18420.17339228545528654No Hit
CCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCATGTCAAAG17930.16877978709084082No Hit
CTCATAGACTCTGGTACTCCTTCCGTAGAAGGCCCTCTTTTCAGACCGCG17800.16755606303496742No Hit
TGTCAAAGGAGGGCACGATCGGGTTCGCTGCCCTTTCGTCCGAGAGCTCG17640.1660499411200464No Hit
CTATTGAATACTGACTTAGCTAATAACGTCCTGCATACTTTCCCAATGGA17580.16548514540195097No Hit
CTCATAAGCAATCCTTGTTTTTCTTCCATTCTCACCCCTCCAGAAGTTTC17200.1619081058540135No Hit
AGCAGGTACTGATTCGAAATGGAAGATTTTGTGCGACAATGCTTCAATCC16770.15786040320766315No Hit
CGTTTAAGGAGACGATAAATGCATTTGAAAAAAAGGCGATCAAGAATCCA16650.15673081177147236No Hit
ATATACAACAGGATGGGGGCTGTGACCACCGAATCGGCATTTGGCCTAAT16530.15560122033528156No Hit
CATTATGATCTGGCATTCCAATTTGAATGATACAACTTACCAGAGGACAA16250.15296550698416972No Hit
TAATAGTAGAGCCTGAAGACCCAAACGCACTTTTGAAGCACAGATTTGAA15860.14929433481654963No Hit
CATATTAAGAGGGTCAGTTGCTCACAAGTCCTGCCTGCCTGCCTGTGTGT15850.14920020219686705No Hit
ATTGAATACTGACTTAGCTAATAACGTCCTGCATACTTTCCCAATGGAAC15720.1479764781409937No Hit
CCCATATACAAGAGAGTAGATGGAAAGTGGATGAGGGAACTTGTCCTTTA15710.14788234552131116No Hit
AATATGGACAGAGCCGTTAAACTGTATAGAAAGCTTAAGAGGGAGATAAC15430.1452466321701993No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAGCTATCTCAA14720.13856321617273712No Hit
GTTGTATATGAGGCCCATACAACTGGCAAGTGCACCAGCAGAATAACTGA14660.13799842045464172No Hit
CTTTTCAGACCGCGTTTAAGGAGACGATAAATGCATTTGAAAAAAAGGCG14480.13630403330035554No Hit
GTATAGAAAGCTTAAGAGGGAGATAACATTCCATGGGGCCAAAGAAATAG13960.1314091370768621No Hit
GCTTATGAGAGAATGTGCAACATTCTCAAAGGGAAATTTCAAACAGCTGC13450.12660837347305123No Hit
CACTTGGAAGTATGCTTCAGATTTTCATTTCATCAATGAGCAAGGCGAGT12700.1195484269968588No Hit
CTATGGTATCAAGTACTCTTGAACTGAGAAGCAGGTACTGGGCCATAAGG12690.11945429437717624No Hit
GTCCCTAAGAGCCTGAACAATAAGGAGCAATTTTCTTGATTCAGCTGAAA12520.1178540398425726No Hit
GTTGTAAAATGAAATGGGGAATGGAGATGAGGCGTTGCCTCCTCCAATCA12280.11559485697019102No Hit
GTATGGAGAGGATCTGAAAATCGAAACAAACAAATTTGCAGCAATATGCA12240.11521832649146076No Hit
GATTTGAAATAATAGAGGGAAGAGATCGCACAATGGCATGGACAGTTGTA11620.10938210407114167No Hit
ATACCATAGTATCCATGTTTTCATTTGAAGCAATTTGTACTCCTCTAGTG11590.10909970621209397No Hit
GTTCCAGATTGTCCCTAAGAGCCTGAACAATAAGGAGCAATTTTCTTGAT11570.10891144097272883No Hit
ATCTCAATGCATGTGTTAGGAAGGAGTTGAACCAAGAAGCATTAAGCAAA11430.10759358429717292No Hit
GAACAATAAGGAGCAATTTTCTTGATTCAGCTGAAAATCCTTCTAATTGT11410.10740531905780779No Hit
GTTTTTTACTCTAGCTCTATGCTGACAAAATGACCATCGTCAGCATCCAC11350.10684052333971238No Hit
TCATTACTCATGTCAAAGGAGGGCACGATCGGGTTCGCTGCCCTTTCGTC11240.10580506452320416No Hit
GTCGTACTCCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCA11150.10495787094606107No Hit
CGATAAATGCATTTGAAAAAAAGGCGATCAAGAATCCACAATATCAAGTG11130.10476960570669593No Hit
GCTTCATATAGCCCCCCAAGATCAAAGGTCCCAGGTTCCAGATTGTCCCT11010.10364001427050513No Hit
GTATTAATGTATACCCCCTTCATTATATATTCTGTGGCTCTGCAATGAGA10960.10316935117209232No Hit
ATATAGTGGTATTCCCTCTCCTTCATGACTTGGATCCTCAATGCTTAATT10690.10062777044066304No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTCG100.0070873876145.0242145
GCACGGG203.8635588E-4108.76815145
AGCGAAA607.2759576E-1296.682811
AACCGCG259.380689E-487.01451145
TAGGGGC353.4345307E-582.87097145
GGCAGCC300.001934494472.5121145
TCGGTCC300.001934494472.5121145
GTAGAAA5700.071.239951
GGGTAGA1550.056.13841
AGTCCCC400.00604699754.384075145
TGGACAC400.00604699754.384075145
GTACTGA4450.053.7704856
ATATTCA1900.049.6112064
CAAGGGT3250.049.08284
GAGCCCC604.941153E-448.341404145
GTTATGT1501.0913936E-1148.3391276
GGTCGTT901.3238496E-648.33237
TTATGTG1501.0913936E-1148.33237
TATGTGC1501.0913936E-1148.33238
TACTGAT4850.047.8340237
Produced by FastQC (version 0.11.5)