FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n01_S1_R1_C04_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n01_S1_R1_C04_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1071740
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATTAGAAGAGGCCTTATTTTCTCAATTTTCTTCTTTGTTGATTCATTG28520.26610931755836303No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT26540.2476346875174949No Hit
CCACTATATGATGCAATCAAATGCATGAAGACATTCTTTGGCTGGAAAGA24880.23214585627111053No Hit
GAATAAGAGAGGCTATCTAATAAGAGCACTGACATTAAATACGATGACCA21880.20415399257282552No Hit
CCATTATATGATGCAATCAAATGCATGAAGACATTCTTTGGCTGGAAAGA21570.20126149999066936No Hit
ATATAGTGGTATTCCCTCCCCCTCGTGACTCGGGTCTTCAATACTTAATT21180.19762255770989232No Hit
ATATAATGGTATTCCCTCCCCCTCGTGACTCGGGTCTTCAATACTTAATT19850.1852128314703193No Hit
CATATAGACTGTTGAATACAGATTTTGCCAGTAAGGTCCTGCACACTTTC19360.18064082706626608No Hit
GTATATTGTCTTGGTGTATTTCTTTTGTCCAAGATTCAGTATCGAGACTC19240.17952115251833467No Hit
AAATAAGACAGGGACATTTGAATTCACAAGCTTTTTTTATCGCTATGGAT18320.17093698098419394No Hit
AACTTATACAATATACGGAATCTTCACATTCCTGAAGTCTGCTTAAAATG17490.16319256536100174No Hit
TTATAGGTGCCATGCTCAGGATGTTTCTGAACCACTCGGGTTGATTTCTG17480.16309925914867412No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA17280.16123313490212177No Hit
CTCTTATTCAGTCTTTGTTTTTTCTTCCCTATTGTTCTTTGCGTGACCAT17250.16095321626513895No Hit
TCCTTAGACAGAATCCAACTGAGGAACAAGCCGTAGACATATGCAAGGCA17210.16057999141582846No Hit
GTATAAGTTTGGTCCTCCATCTGATACTAATAGCCCTACCTTTGATTGGG17020.15880717338160374No Hit
GTCTAAGGATGTCCACCATCCTTACTCCTCCAATCTGTGTGCTGTGGCAC15580.14537107880642694No Hit
GTATAACATTGTGGGATCTTGTGACCATTGAATTTTCACAATTTCCCAGT15460.14425140425849553No Hit
CTTCTAATAGATGGCACAGCATCACTGAGTCCTGGGATGATGATGGGCAT15210.1419187489503051No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC14750.13762666318323474No Hit
ATATAAACACTGCCTGTTCCGCCGGCTACTGGGAGAAACCTTGTTTTACG14670.1368802134846138No Hit
TCCCAGGACTCAGTGATGCTGTGCCATCTATTAGAAGAGGCCTTATTTTC14460.13492078302573388No Hit
CATTAAATACGATGACCAAAGATGCAGAGAGAGGCAAGTTAAAAAGAAGG14180.13230820908056057No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT14170.13221490286823295No Hit
GATTAGAGGTTTCGTATACTTTGTTGAAACTTTAGCTAGGAGCATTTGCG14110.13165506559426726No Hit
TTCGTATACTTTGTTGAAACTTTAGCTAGGAGCATTTGCGAAAAGCTTGA14070.1312818407449568No Hit
CTTTATGACTGACAAAGGGATTCAGGGGATTACAAAGTCTTCCCCGATAA13970.13034877862168065No Hit
CATATAGGTGCCATAGGGGAGACACACAAATTCAGACGAGAAGATCATTT13510.12605669285461024No Hit
ACACTAAGTGGAATGAAAATCAAAATCCTCGAATGTTCCTGGCGATGATT13310.12419056860805792No Hit
CCCATAATGTTCTCAAACAAAATGGCAAGACTAGGGAAAGGGTACATGTT13270.12381734375874745No Hit
TTCCTGAAGTCTGCTTAAAATGGGAGCTAATGGATGATGATTATCGGGGA13100.12223113814917798No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG12820.1196185642040047No Hit
ATGTTATACAACAAAATGGAATTTGAACCATTTCAGTCTCTTGTCCCTAA12510.11672607162184859No Hit
ACTTAGTGTTGTCCCCAGTGATTGTGAAAGAAATCTCTGTGTCTTGTGAA12020.11215406721779536No Hit
CATTATGGGTGCCATGCTCAGGATGTTTCTGAACCACTCGGGTTGATTTC11730.10944818706029448No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTG11690.109074962210984No Hit
GTACTATTGTCTCCCGAAGAAGTCAGTGAAACGCAAGGAACTGAGAAGTT11200.10450295780693078No Hit
CACATATAGGTGCCATAGGGGAGACACACAAATTCAGACGAGAAGATCAT11030.10291675219736131No Hit
TATTTAATGTCAGTGCTCTTATTAGATAGCCTCTCTTATTCAGTCTTTGT10740.10021087203986041No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTTG100.0070875366145.02324145
GTTACCT100.0070875366145.023241
TATAAGC100.0070875366145.02324145
CAGGTAC1900.0103.042833
GCAGGTA1950.0100.40072
AGTAGAA3150.098.9841161
TTCGGCG259.380959E-487.01394145
TCTCCGC451.2317723E-680.56847145
GGTACTG2500.078.3125465
GTAGAAA4650.067.053762
AGGTACT2950.066.366574
ATATAAT5600.066.037371
ATTATAT6350.063.9472583
GCCCACC606.7994642E-660.426346145
TTATATG6950.058.426634
TGATGCA13750.056.9439549
AGCGAAA651.0926604E-555.7781641
CCACTAT7400.054.873661
ACTATAT7650.053.0804023
AGCGAGC553.2165603E-452.73572145
Produced by FastQC (version 0.11.5)