FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n01_S1_R1_C01_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n01_S1_R1_C01_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTCATGTATTCGGATTTCCATTTCATCGACGAACGGGGTGAATCAATA13.571428571428571No Hit
CGCGTGAGGCATTTCCAAAAAGATGCAACAGTGCTTTTCCAGAACTGTGG13.571428571428571No Hit
ATTTTAAGCAGACTTCAGGAATGTGAAGATTCCGTATATTGTATAATTTT13.571428571428571No Hit
GTGCATCCTCAAGAATTCCCCTTTGGATTGTGTGGAGAATAGAACGATTC13.571428571428571No Hit
GAAAAGAACATACTACTGAAAGCGAACTTCAGTGAAAACTTAAACCGATT13.571428571428571No Hit
GTAGGATGCAATTTTCCTCATTGACTGTGAATGTGAGAGGATCAGGGTTG13.571428571428571No Hit
CTTCTATGGTGGAACAGATCTTCATGATCTCACAGAAGTCTAGTTTCTTG13.571428571428571No Hit
GCACTACTGACGCGTGGATTGGATCAAACAGGGTGTTAACCTCAATATCT13.571428571428571No Hit
GATCATGAGTCCAGATTCAAGAATAAGGGCTAACGTCAAAACTCCCTGTA13.571428571428571No Hit
CAATAAGGCATGTGAATTGACTGATTCAAGCTGGATATAACTTGATGAAA13.571428571428571No Hit
CAATATCAGCACACAAATGAGATAATACAATGAAAAGCAAGAAAAAAAGC13.571428571428571No Hit
CACACACCCACTCACACAACTTCATCTTCACTACCTCTCCTCTCTCCTCA13.571428571428571No Hit
GGTTTAGATCTGCGTCGATATGGTCTGCACACTATTGAGTGGCGAGAATC13.571428571428571No Hit
GTCCCTAAGTGCCTGAACAATGAGAAGCAATTTTCTAGATTCAGCCGAAA13.571428571428571No Hit
GTATAAGTTTGGTCCTCCATCTGATACTAATAGCCCTACCTTTGATTGGG13.571428571428571No Hit
AATCGAAACTATCAAGTTTGCTGCAATATGCACACATTTGGAATTTTGTT13.571428571428571No Hit
GGATTGCCTGATCACGGCAGTTAGGGGCGATGTGAACTTTGTCAATAGGG13.571428571428571No Hit
GAAGTATGCTTCAGATTTTCATTTCATCAATGAGCAAGGCGAGTCAATAA13.571428571428571No Hit
TCTCTTGTCTTCTTTGCCCAAAATGAGAAATCCTCTCAGGACAGCAGACT13.571428571428571No Hit
CCCAATTAGCACATTAGCCTTCTCTACTTTTGCACGATTGAGCAGTTCAT13.571428571428571No Hit
CCCTTATACTGGAGATCCTCCATACAGCCAATCCTGTGTTGAACCTTTAG13.571428571428571No Hit
GACTAAAGATAAAAATATAGGTCCCAAACTGGACACACAAAACGACAGAA13.571428571428571No Hit
ACCCTGGCATCAGAAATTCCAACCGGTCTCCTATATGAACTGCTAGGGAA13.571428571428571No Hit
GAGGATAATGAAAAAAGTTGGTATGCACAAACAGCCTGTGTTCTAGACGC13.571428571428571No Hit
CCCATAGACTCCTACTGGCCATTTCGTGTCTTATAGTGAAAAGCCTAGTT13.571428571428571No Hit
GTATCGAGCCTCCCAAGACCGTACTTAGCATGTTGAACATGCCCATCATC13.571428571428571No Hit
ACATAATTCAGTTTAAAAACCGCAATAGCCTGTAAGTCAACTGCTCAGAA13.571428571428571No Hit
ATCCTCCATACAGCCATGGAACAGGAACAGGATACACCATGGACACAGTA13.571428571428571No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)