FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n01_S1_R1_C01-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n01_S1_R1_C01-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1045302
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTAAACTATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG62450.5974349996460353No Hit
ATTATATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATGTCGCA60380.5776321101461588No Hit
GAATAAAAGAACTAAGAAATCTAATGTCGCAGTCTCGCACCCGCGAGATA33700.3223948677033049No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT23410.2239544170010198No Hit
ATCTAATGTCGCAGTCTCGCACCCGCGAGATACTCACAAAAACCACCGTG22160.21199615039481412No Hit
GTAGAAACAAGGTCGTTTTTAAACTATTCGACACTAATTGATGGCCATCC20450.19563724167752478No Hit
GGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATG20310.19429791581762973No Hit
CCATATGGCCATAATCAAGAAGTACACATCAGGAAGACAGGAGAAGAACC17160.16416308396999144No Hit
AATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGT16720.15995377412460704No Hit
AAACAAGGTCGTTTTTAAACTATTCGACACTAATTGATGGCCATCCGAAT15660.14981316404254463No Hit
ACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG14090.1347935811851503No Hit
ATCAAGAAGTACACATCAGGAAGACAGGAGAAGAACCCAGCACTTAGGAT13620.13029727294121698No Hit
ATATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATGTCGCAGTC13030.1246529711030879No Hit
ATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATGTCGCAGTCTC11950.11432102875532622No Hit
GTCTCGCACCCGCGAGATACTCACAAAAACCACCGTGGACCATATGGCCA11760.11250337223118297No Hit
CATATATACTTACACAGTTTGAGCATTCACAGGCCACTAGAAGCCCATCT10510.10054510562497727No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA2450.065.11071
ATTATAT17300.060.773971
TATATTC18500.058.3996543
CTATTCG16150.056.5628749
TTATATT19200.055.5151942
GTCGTAT400.00604778154.3822251
ATATTCA19700.053.7381064
TATTCAA20300.052.864175
TCAATAT19850.051.4981968
ATTCAAT20900.049.9587976
CAATATG21650.048.2212039
ACTATTC19650.047.963878
TTCAATA21700.047.7759937
TAAACTA20450.046.8032885
GGTCAAT6400.046.4514851
TATACGT1602.0008883E-1145.3185234
GTCAATT6300.044.886922
TTAAACT21650.044.544034
AACTATT21200.043.7731257
AAACTAT21950.042.6138656
Produced by FastQC (version 0.11.5)