FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n01_S1_R1_B09_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n01_S1_R1_B09_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences86
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTATATGCGTCTCCACAACTTGAGGGGTTTTCGGCTGAATCTAGAAAA22.3255813953488373No Hit
CCATGGACACAGTAAACAGAACACACCAATACTCAGAAAAGGGAAAGTGG22.3255813953488373No Hit
GAATAAGTTATTGTCAACTTCTCAGTTCCTTGCGTTTCACTGACTTCTTC11.1627906976744187No Hit
GGATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCGCAAAATGCCA11.1627906976744187No Hit
GAGATATGGACCAGCATTAAGCATCAATGAACTCAGCAATCTTGCAAAAG11.1627906976744187No Hit
AATAAGAGCACGGCCATTAAATACGATGACCAAAGATGCAGAGAGAGGCA11.1627906976744187No Hit
GACTTATGATTGGACATTAAACAGAAATCAACCGGCAGCAACTGCATTGG11.1627906976744187No Hit
GGATTGGAGTAACAGTGATAAAGAACAACATGATAAACAATGACCTTGGA11.1627906976744187No Hit
ATATCCTTCTCAGTTAAAGGGTAATTGAGTCCAAGCTTTCCAAATTCTGA11.1627906976744187No Hit
ATACAGGTTTGTTTTCCGTCTTCCTTCTTTGGTCCTACATTTGCTTATCA11.1627906976744187No Hit
GTCGGGCAGTATTCCGATCATTCCCATCACATTGTCGATGGATTCAATTC11.1627906976744187No Hit
GTGCTCTCTAATGCAAGGTTCAACACTTCCCAGAAGGTCTGGTGACGCAG11.1627906976744187No Hit
GGCACTCCTTCCGTAGAAGGCCCTCTTTTCACACCGTATTTAAAGAGAAG11.1627906976744187No Hit
CCCTTACACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA11.1627906976744187No Hit
CTGGGCAGCTGTTTGAAATTTTCCTTTGAGGATATTGCACATTCTTTCAT11.1627906976744187No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTAGCGAGATA11.1627906976744187No Hit
CTATTGAAGCACCGATCTATTGGGAAAGTGTGCAGGACCTTACTGGCAAA11.1627906976744187No Hit
GGTTCGTTGCCTTTTCGTCCGAGAGCTCGAAGACTCCCCGCCCCTGGAAG11.1627906976744187No Hit
AACTTATACAATATACGGAATCTTCACATTCCTGAAGTCTGCTTAAAATG11.1627906976744187No Hit
AGCGAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAG11.1627906976744187No Hit
GAATGTGCAATATCCTCAAAGGAAAATTTCAAACAGCTGCCCAGAGGGCA11.1627906976744187No Hit
GGTGGGGCAGCAGCAAAGGGGAGAAGTTTTATTATTTGGACAGTGTCAAA11.1627906976744187No Hit
GGATTTATGTGCACCTGATCCCCTCAGAATGAGTGATCAGCGTGCCAGGA11.1627906976744187No Hit
ATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCC11.1627906976744187No Hit
ACATTTATGATAAAGGGAAAGAAGTAATCTTGCTATGGGGCATTCACGAT11.1627906976744187No Hit
TCATTAATCAGGCACTCCTCGATTGCTTCATATAGCCCCCCAAGATCGAA11.1627906976744187No Hit
GTCCTGCACACTTTCCCAATAGAGACTTCCACCACTCCCCGCGGTGACTC11.1627906976744187No Hit
ATTTCATCAAGTTCTATCCAGCTTGAATCAGTCAATTCACATGCCTTATT11.1627906976744187No Hit
CACTAAGACCACTGTGTACCATATGGCCATAAAAAAAAAGTACACATCAG11.1627906976744187No Hit
CCACTGTCCAGGCCATGATTCGGTCTCTTCCTTCAATTATCTCAAATCGG11.1627906976744187No Hit
ATCCACACCCCACCCCCAGCCCATTTTATGTCTCAATTTGGCAAAAGAAA11.1627906976744187No Hit
AGTAGAAAAAAGGCATTTTTTCATGAAGGACAAGTTACATTCATGATTTT11.1627906976744187No Hit
CATTTCTTCATTTAACCCCCTTATTTCCTCAAATTTCTGTCCCAATTGCT11.1627906976744187No Hit
GTGTGCCACTTGTGAACAGATTGCTGATTCACAGCATCGGTCTCACAGAC11.1627906976744187No Hit
CACATTTGGAAGTTTGTTTCATGTATTCGGATTTCCATTTCATCGACGAA11.1627906976744187No Hit
CATTTCTTCAATTAACCACCTTATTTCCTCAAATTTCTGTCCCAATTGCT11.1627906976744187No Hit
ATGCTCTTGTATTTGTGGCGGTAGGTTGTCGCATCTGCAGGAGATCTAAT11.1627906976744187No Hit
CTACCTGCATGGAATATGATGCCGTTGCAACTACACATTCCTGGATTCCC11.1627906976744187No Hit
GGTATATAAAACTTATTTCGAAAAGGTCGAAAGGTTGAAACATGGTACCT11.1627906976744187No Hit
CAAATGTCCCAAGCACATCCCGCATTTGCTGGAACAGTGTCCTTACGAAT11.1627906976744187No Hit
GCATTAGAGAGCACATTCTGGGATCCATTCCGGTGCGAACAAGCGCTCTT11.1627906976744187No Hit
AAAGAAATGAGAGAAATAATGTCGCAGTCCCGCACTCGCGAGCTACACAC11.1627906976744187No Hit
GTCAATAAGGCATTCAAGAGACTCACACCACTTTACATAAAATTTATTAA11.1627906976744187No Hit
CCCCTCAAGTTGTGGAGACGCATATAGACTGTTGAATACAGATTTTGCCA11.1627906976744187No Hit
AGTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGAT11.1627906976744187No Hit
CGGTTAAAGATTACACTGAAGTTCGCTTTCAGTACTATGTTCTTTTCCAT11.1627906976744187No Hit
ATCTGCTTCTCAGTTCCAGGGTATTCGAGTCCATGGTTTCCACATTGTCT11.1627906976744187No Hit
GAAGATGGTCACGCAAAGAACAATAGGGAAGAAAAAACAAAGACTGAATA11.1627906976744187No Hit
GCTTCATATAGGCCCCCAAGATCGAAGGTTCCAGGATCCAGGTTGTCCCT11.1627906976744187No Hit
GTTTTATATACATTACGGTAATGAACTGTACATGTTGTTGGGCCATTCAT11.1627906976744187No Hit
CAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGG11.1627906976744187No Hit
GAACAATAGCAATGGAGTTAATCAGAATGATCAAACGTGGAATCAATGAC11.1627906976744187No Hit
CAATAAGCAAACCACAGGGATCATTAATCAGGCACTCCTCGATTGCTTCA11.1627906976744187No Hit
CGATACACCATGGACACAGTAAACAGAACACACCAATACTCAGAAAAGGG11.1627906976744187No Hit
CCCCAGATGTGCCTTCATCTGGATCTTCAGTCAATGCACCTGCATCCTTT11.1627906976744187No Hit
ATCTAATGTCGCAGTCCAGCACTCGCGAGATACTCACTAAGACCACTGTG11.1627906976744187No Hit
AGATTCAGCCGAAAACCCCTCAAGTTGTGGAGACGCATATAGACTGTTGA11.1627906976744187No Hit
CTTCCAGGACATACTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCAT11.1627906976744187No Hit
TATTCCATGCTTTTGGCTGGACCATGGGCTGGCATTACCACAGCATTGTT11.1627906976744187No Hit
GGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGA11.1627906976744187No Hit
GAGTATTGGTGTGTTCTGTTTACTGTGTCCATGGTGTATCCTGATACTGT11.1627906976744187No Hit
CGATCGGGTTCGTTGCCTTTTCGTCCGAGAGCTCGAAGACTCCCCGCCCC11.1627906976744187No Hit
GCTGCATTTGAAGATTTAAGAGTATCAAGTTTCATAAGAGGAAAGAAAGT11.1627906976744187No Hit
GTTCCAGCAAATGCGGGATGTGCTTGGGACATTTGACACTGTCCAAATAA11.1627906976744187No Hit
GAATGAAGTGGATGATGGCAATGAGATAATGGACATGATTCCAGAGAGGA11.1627906976744187No Hit
AATCCAACCTGAATGATGCCACATATAAGAGGACAAGAGCGCTTGCACGC11.1627906976744187No Hit
CACAGATGGCTACTACAACCAATCAACTAAGCAGTCAACAAAACACAATG11.1627906976744187No Hit
GCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCAAAACCCAGGG11.1627906976744187No Hit
GGATTTTGTTGTATAGCATTGTGGGATCTTGTGACCATTGAATTTTCCCA11.1627906976744187No Hit
GGGCCTTGCTAAATGACAAACATTCCAATGGAACCATTAAAGACAGGAGC11.1627906976744187No Hit
GATGCAATCAAATGCATGAAGACATTCTTTGGCTGGAAAGAGCCTAACAT11.1627906976744187No Hit
ATCCACCAACTAGTGCTGACCAACAAAGTCTCTATCAGAATGCAGATGAA11.1627906976744187No Hit
CATTATGGGTGCCATGCTCAGGATGTTTCTGAACCACTCGGGTTGATTTC11.1627906976744187No Hit
ATTATTGCCTCAGCAATTGACTGCTCGTCTCTCCCGCTTACTATCAACTG11.1627906976744187No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG11.1627906976744187No Hit
AATAGTATCAGATGGAGGACCAAACTTATACAATATACGGAATCTTCTCA11.1627906976744187No Hit
CTTGTGAACAGATTGCTGATTCACAGCATCGGTCTCACAGCCAGATGGCT11.1627906976744187No Hit
ATACAAACACTACGTCCCCTTGCCCAATTAGCACATTAGCCTTATCTCAT11.1627906976744187No Hit
ATGGTACATCTGTTCATCCTCAAGAAATCAACTTTGGCTTGTGTTGAGAA11.1627906976744187No Hit
CTGTCTGGCGGTCAGTAAGTATGCTAGAGTCCCGTTTTGGGGTGATTTCC11.1627906976744187No Hit
ATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTG11.1627906976744187No Hit
GTAATATATGTGGACTTCCCTCCGTGTTACTCCAATTTCAATGAACCGGT11.1627906976744187No Hit
TCGCAGTACCGCACTCGCGAGATACTCACTAAGACCACTGTGGACCATAT11.1627906976744187No Hit
CATTATGGGTGCCATGCTCAGGATGTTTATGAACCACTCGGCTTGAATTC11.1627906976744187No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTGGAG50.0145.03
GGATTGG50.0145.01
TGGAGTA50.0145.05
GATTGGA50.0145.02
GAGTAAC50.0145.07
AGTAACA50.0145.08
GATCATT50.0145.0145
TTGGAGT50.0145.04
GTAACAG50.0145.09
GGAGTAA50.0145.06
Produced by FastQC (version 0.11.5)