FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n01_S1_R1_B08-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n01_S1_R1_B08-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1107142
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTATATACAGCTCGCTGAAGAGGTCCATGACCTCAAAAAAGATCAAATG18560.16763883946232733No Hit
GTATATACATACATATATACTTACACAGTTTGAGCATTCACAGGCCACTA18500.16709690355889306No Hit
CATATATACTTACACAGTTTGAGCATTCACAGGCCACTAGAAGCCCATCT16550.14948398669728002No Hit
GTATATATGTATGTATATACAGCTCGCTGAAGAGGTCCATGACCTCAAAA12650.11425815297405391No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG800.002034586236.25775
AGCGAAA2700.034.9148251
TATACAG12200.032.6913725
TTATACG1153.158379E-431.5284374
GTATAGG4850.029.903261
CAGCTCG10600.029.4099839
CATACAT13200.027.4617528
GTATATA24150.026.4238321
TTAGCGT1701.02990205E-425.5936744
GTATAAG5800.025.0053141
ATATACT13600.024.5272714
TATATAC29150.023.8815753
TACATAC16100.023.871536
TATACTT15850.023.3330335
GTACTAT5950.023.156181
CGAAAGC4403.6379788E-1223.0730823
TACAGCT17300.022.2106867
CGTAGAA2303.137265E-522.0699062
GTATACT3004.5465276E-721.7546223
ATACAGC15200.021.4683766
Produced by FastQC (version 0.11.5)