FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n01_S1_R1_A06-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n01_S1_R1_A06-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences911586
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA1750.049.718141
CAATTCG1850.047.0255139
GCGAAAG3100.046.777821
CGAAAGC3200.045.3160172
CAGTTCG800.00203684236.2488379
CGTTACG1153.1604446E-431.5241831
TATAATA5150.025.341774
CCTTATA3451.07320375E-1025.2193472
TAGAAAC8700.025.0019383
TACGGGT1450.001225805625.0019384
GCGTAAC2101.5539392E-524.1685411
GTTACGC2751.9667459E-723.7291133
TCTCCGC1851.8295311E-423.515339145
TACCTAT3102.4181645E-823.3863457
TATACAG5900.023.3492685
TACGCAG2802.3404027E-723.3053785
ATATACT8750.023.20184
GTATTAG2202.2271879E-523.0699711
TACTATA2603.2014923E-622.3094222
TTACGCA2953.8707003E-722.120364
Produced by FastQC (version 0.11.5)