FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n01_S1_R1_A05_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n01_S1_R1_A05_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAAAACCCAGGGATCATTAATCAGGCACTCCTCGATTGCTTCATATA15.263157894736842No Hit
GGTTGGGAGAAGAGCAACAGCTATTCTCAGAAAGGCAACCAGGAGATTGA15.263157894736842No Hit
CATATAGACTGTTGAATACAGATTTTGGCAGTAAGGTCCTGCAGACTTTC15.263157894736842No Hit
CCGCTTGTCCTTTTTAAAGTGAACCAACAAAAACTGAAAGATGAGCTAAT15.263157894736842No Hit
CTGTCATACTGTTTAAGGTCTCCAACATCTTTGCAGTCATCAAATTCTAC15.263157894736842No Hit
GATTGGGTGATGCCCCATTCCTTGATCGGCTCCGCCGAGATCAAAAGTCC15.263157894736842No Hit
GTTCGAATCTTCATTCTTTTACTCTCGAACATGTACCCTTTCCCTAGTCT15.263157894736842No Hit
CGGCCGGTCGGCGGCCTCGCCTGACCACTGCTCAGGGTGAGTGGGAGCAC15.263157894736842No Hit
GGCTCAGGGTGGGCAGACATATGATACGACTATAAAGACATTTGATCGGG15.263157894736842No Hit
GAGCTACATCTTCTCCAATCTCATCGAGCTCTATCCAGATTGAATCGGTC15.263157894736842No Hit
GGGTAGCTACATGCTAAAATTAGCCAAAAATCAATAGCGATAAAAAAAGC15.263157894736842No Hit
AGAAAGCTCTTATCTCTTGTTCTACTTCAAGCAGTAGTTGTAATGCTTGC15.263157894736842No Hit
CAACATGATAAACAATGACCTTGGACCTGCAACGGCCCAGATGGCTCTTC15.263157894736842No Hit
ATACTCACTACCACTCTCTCCGTGCTGGAGTATTCATCTACTCCCATTTT15.263157894736842No Hit
ACCATCAACGGAGCAATTTTACAATCCTGGAGCTCTTCTTTCTTCTCTTT15.263157894736842No Hit
GTACTTGTTGTTGGGCCATTCCTATTCCACAAAGGAACAGCCAGAGGTGA15.263157894736842No Hit
ATTGTGGCCATGGTATTCTCACAGGAGGATTGCATGATCAAGGCAGTTAG15.263157894736842No Hit
AAAGAACATAGTACTGAAAGCGAACGTAAGTGTAATCTTTAAACGATTAG15.263157894736842No Hit
ATATAAACACTGCCTGTTCCGCCGGCTACTGGGAGAAACCTTCTTTTACG15.263157894736842No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)