FastQCFastQC Report
Thu 15 Jul 2021
HTGNWAFX2_n02_BS_doli_lib_04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGNWAFX2_n02_BS_doli_lib_04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36751887
Sequences flagged as poor quality0
Sequence length151
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1145330.31163842008983106No Hit
GTCCTTTCGTACTAAAATATTAACCTATATTTAAAGATAGAAACCAACCT849130.23104391891496617No Hit
GCGATAATGAGATTTAATTAACTCTCATAATAAATTAACATAGATTAATG798570.21728680217154564No Hit
GCCGGTTTGAACTCAGATCATGTAAGAAATTAAGAGTCGAACAGACTCAA661840.18008327028214904No Hit
CAGAATTTTAACTGTGCAAAGGTAGCATAATCATTAGTCTTTTAATTGAA562660.15309690084756736No Hit
ATTTAATTAACTCTCATAATAAATTAACATAGATTAATGAATAAATGATC544720.1482155188385293No Hit
CCAACATCGAGGTCGCAATCTATTTTATCGATATGAACTCTCCAAAATAA540810.1471516278878415No Hit
CTAAAATTAATCTTAGTCCAACATCGAGGTCGCAATCTATTTTATCGATA530050.14422388706190786No Hit
TTTTAGCCTTTTAACTAAAAAGTAAAATTCTAATTATCCAATTGAGACAG518220.14100500472261465No Hit
AAATGATCCATTTTTATTGATTAAAAATTTAAGTTACCTTAGGGATAACA462250.1257758547200583No Hit
TCGCAATCTATTTTATCGATATGAACTCTCCAAAATAATTACGCTGTTAT436890.11887552875856416No Hit
CTTTTAACTAAAAAGTAAAATTCTAATTATCCAATTGAGACAGTATATAT428610.1166225832159312No Hit
TTTTAATTGAAGGCTTGTATGAATGATTGGACGAGATATATACTGTCTCA425860.11587432231710987No Hit
TTTAGCCTTTTAACTAAAAAGTAAAATTCTAATTATCCAATTGAGACAGT405700.11038888969156876No Hit
AAAATATTAACCTATATTTAAAGATAGAAACCAACCTGGCTCACGCCGGT376900.10255255736936718No Hit
CTAAAATATTAACCTATATTTAAAGATAGAAACCAACCTGGCTCACGCCG369690.1005907533400938No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)