FastQCFastQC Report
Thu 15 Jul 2021
HTGNWAFX2_n02_BS_doli_lib_03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGNWAFX2_n02_BS_doli_lib_03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35471410
Sequences flagged as poor quality0
Sequence length151
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1255150.3538483528001847No Hit
GTCCTTTCGTACTAAAATATTAACCTATATTTAAAGATAGAAACCAACCT801200.22587204737561883No Hit
GCGATAATGAGATTTAATTAACTCTCATAATAAATTAACATAGATTAATG794310.22392963798168725No Hit
GCCGGTTTGAACTCAGATCATGTAAGAAATTAAGAGTCGAACAGACTCAA655700.18485309718446488No Hit
CAGAATTTTAACTGTGCAAAGGTAGCATAATCATTAGTCTTTTAATTGAA526390.1483983861932751No Hit
CCAACATCGAGGTCGCAATCTATTTTATCGATATGAACTCTCCAAAATAA513210.14468271771547847No Hit
TTTTAGCCTTTTAACTAAAAAGTAAAATTCTAATTATCCAATTGAGACAG496170.13987884890958663No Hit
ATTTAATTAACTCTCATAATAAATTAACATAGATTAATGAATAAATGATC494870.13951235657110897No Hit
CTAAAATTAATCTTAGTCCAACATCGAGGTCGCAATCTATTTTATCGATA492900.13895697971972357No Hit
TCGCAATCTATTTTATCGATATGAACTCTCCAAAATAATTACGCTGTTAT460000.12968190438440425No Hit
AAATGATCCATTTTTATTGATTAAAAATTTAAGTTACCTTAGGGATAACA448470.1264313992592908No Hit
TTTAGCCTTTTAACTAAAAAGTAAAATTCTAATTATCCAATTGAGACAGT382130.10772901330959216No Hit
CTTTTAACTAAAAAGTAAAATTCTAATTATCCAATTGAGACAGTATATAT372720.10507617261338074No Hit
TTTTAATTGAAGGCTTGTATGAATGATTGGACGAGATATATACTGTCTCA367320.10355381982278121No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)