FastQCFastQC Report
Thu 15 Jul 2021
HTGNWAFX2_n01_BS_doli_lib_04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGNWAFX2_n01_BS_doli_lib_04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36751887
Sequences flagged as poor quality0
Sequence length151
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTTTCGTACTAAAATATTAACCTATATTTAAAGATAGAAACCAACCT835060.22721554406172395No Hit
GCGATAATGAGATTTAATTAACTCTCATAATAAATTAACATAGATTAATG796410.21669907724738052No Hit
GCCGGTTTGAACTCAGATCATGTAAGAAATTAAGAGTCGAACAGACTCAA619610.16859270382497638No Hit
CAGAATTTTAACTGTGCAAAGGTAGCATAATCATTAGTCTTTTAATTGAA597210.16249777868548626No Hit
ATTTAATTAACTCTCATAATAAATTAACATAGATTAATGAATAAATGATC558000.15182893874265557No Hit
CCAACATCGAGGTCGCAATCTATTTTATCGATATGAACTCTCCAAAATAA557140.1515949371524787No Hit
CTAAAATTAATCTTAGTCCAACATCGAGGTCGCAATCTATTTTATCGATA548220.14916785089157464No Hit
TTTTAGCCTTTTAACTAAAAAGTAAAATTCTAATTATCCAATTGAGACAG514650.1400336260285084No Hit
AAATGATCCATTTTTATTGATTAAAAATTTAAGTTACCTTAGGGATAACA478840.13028990865149317No Hit
TCGCAATCTATTTTATCGATATGAACTCTCCAAAATAATTACGCTGTTAT458970.1248833835389187No Hit
TTTTAATTGAAGGCTTGTATGAATGATTGGACGAGATATATACTGTCTCA441670.12017614224815179No Hit
CTTTTAACTAAAAAGTAAAATTCTAATTATCCAATTGAGACAGTATATAT417910.11371116808233549No Hit
TTTAGCCTTTTAACTAAAAAGTAAAATTCTAATTATCCAATTGAGACAGT399980.10883250702202041No Hit
CTAAAATATTAACCTATATTTAAAGATAGAAACCAACCTGGCTCACGCCG381050.10368175108940664No Hit
AAAATATTAACCTATATTTAAAGATAGAAACCAACCTGGCTCACGCCGGT380950.10365454160217678No Hit
TAAATATTGAATGGCCGCAGAATTTTAACTGTGCAAAGGTAGCATAATCA377050.102593371600212No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)