FastQCFastQC Report
Thu 15 Jul 2021
HTGNWAFX2_n01_BS_doli_lib_03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGNWAFX2_n01_BS_doli_lib_03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35471410
Sequences flagged as poor quality0
Sequence length151
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGATAATGAGATTTAATTAACTCTCATAATAAATTAACATAGATTAATG794440.223966287215535No Hit
GTCCTTTCGTACTAAAATATTAACCTATATTTAAAGATAGAAACCAACCT791900.22325021756958632No Hit
GCCGGTTTGAACTCAGATCATGTAAGAAATTAAGAGTCGAACAGACTCAA601040.16944350393739635No Hit
CAGAATTTTAACTGTGCAAAGGTAGCATAATCATTAGTCTTTTAATTGAA561270.15823165755181426No Hit
CCAACATCGAGGTCGCAATCTATTTTATCGATATGAACTCTCCAAAATAA533590.15042818991407447No Hit
CTAAAATTAATCTTAGTCCAACATCGAGGTCGCAATCTATTTTATCGATA510360.14387925374266206No Hit
ATTTAATTAACTCTCATAATAAATTAACATAGATTAATGAATAAATGATC506410.14276568086805683No Hit
TTTTAGCCTTTTAACTAAAAAGTAAAATTCTAATTATCCAATTGAGACAG492690.13889777711120027No Hit
TCGCAATCTATTTTATCGATATGAACTCTCCAAAATAATTACGCTGTTAT482350.13598275343438562No Hit
AAATGATCCATTTTTATTGATTAAAAATTTAAGTTACCTTAGGGATAACA464740.1310181918339305No Hit
TTTTAATTGAAGGCTTGTATGAATGATTGGACGAGATATATACTGTCTCA388110.1094148780665894No Hit
TTTAGCCTTTTAACTAAAAAGTAAAATTCTAATTATCCAATTGAGACAGT377170.10633070407970813No Hit
CTTTTAACTAAAAAGTAAAATTCTAATTATCCAATTGAGACAGTATATAT362560.10221189402958607No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)