FastQCFastQC Report
Thu 15 Jul 2021
HTGNWAFX2_n01_BS_doli_lib_02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGNWAFX2_n01_BS_doli_lib_02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32952340
Sequences flagged as poor quality0
Sequence length151
%GC33

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC1228190.37271708170041945TruSeq Adapter, Index 6 (100% over 50bp)
GTCCTTTCGTACTAAAATATTAACCTATATTTAAAGATAGAAACCAACCT401260.12176980451160677No Hit
GCGATAATGAGATTTAATTAACTCTCATAATAAATTAACATAGATTAATG372260.11296921553977654No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)