FastQCFastQC Report
Thu 15 Jul 2021
HTGNWAFX2_n01_BS_doli_lib_01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGNWAFX2_n01_BS_doli_lib_01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28782585
Sequences flagged as poor quality0
Sequence length151
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTTTCGTACTAAAATATTAACCTATATTTAAAGATAGAAACCAACCT365670.12704557286984472No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGTCGTATGC346790.1204860508533198TruSeq Adapter, Index 5 (98% over 50bp)
GCGATAATGAGATTTAATTAACTCTCATAATAAATTAACATAGATTAATG339330.117894205819248No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)