FastQCFastQC Report
Thu 13 May 2021
HTGM5AFX2_n02_WT03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGM5AFX2_n02_WT03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15652406
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATAGAAGAACT564960.3609413147090613No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG514710.3288376240687853No Hit
AAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAA366060.23386819892098376No Hit
AGTATGGCCCGGGGGATCCTACGTTCCAAATGCAGCGAGCTCGTATAACC357190.2282013385034863No Hit
GTACTGGATGCATCTGCAGGATATCGCGGCCGCTCGACGTAGAACTCAAT342420.21876508953320017No Hit
GAAAAATTATAACCAAGCATAATATAGCAAGGACTAACCCCTATACCTTC301650.19271797575401506No Hit
GATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATA246070.15720905782791475No Hit
CTCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCT244480.156193239556909No Hit
GTACTGGATGCATCTGCAGGATATCGCGGCCGCCATCTGCCCTACGTTTG238420.1523216302976041No Hit
AGTATGGCCCGGGGGATCCTTATCTGTCAAAACCGCTAATGTCCGTTCTA237950.15202135697221245No Hit
CAAACATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCT234430.14977250142885382No Hit
AAAAAATCCCAAACATATAACTGAACTCCTCACACCCAATTGGACCAATC212970.13606214916735485No Hit
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTAC211980.13542965854578523No Hit
CCCAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAG211500.13512299642623632No Hit
CCCAAACATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCACC206830.13213942955479177No Hit
CTTGTAGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC193260.12346983588337793No Hit
ATTGAAACCTGGCGCAATAGATATAGTACCGCAAGGGAAAGATGAAAAAT183090.11697243222543549No Hit
CTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACCCAAATAAAG179370.11459580079893147No Hit
GGAGGATCGCTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGAT173090.11058363806816665No Hit
AGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATAT172410.11014920006547235No Hit
CGTGATCTGAGTTCAGACCGGAGTAATCCAGGTCGGTTTCTATCTACTTC163480.10444400688303128No Hit
GGAAAGATGAAAAATTATAACCAAGCATAATATAGCAAGGACTAACCCCT163030.10415651114595419No Hit
CTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAATTGAAAC161690.10330041272888013No Hit
GCTCTTTGGACACTAGGAAAAAACCTTGTAGAGAGAGTAAAAAATTTAAC160940.10282125316708499No Hit
TAGAAATTGAAACCTGGCGCAATAGATATAGTACCGCAAGGGAAAGATGA157650.10071933988934353No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)