FastQCFastQC Report
Thu 13 May 2021
HTGM5AFX2_n02_N1_02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGM5AFX2_n02_N1_02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10180406
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG207510.20383273515810668No Hit
CTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATAGAAGAACT202470.19888204851555036No Hit
CTCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCT146590.14399229264530314No Hit
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTAC109000.10706842143623742No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)